Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2761 and Shew_2762 are separated by 91 nucleotides Shew_2762 and Shew_2763 overlap by 8 nucleotides Shew_2763 and Shew_2764 are separated by 60 nucleotides
Shew_2761: Shew_2761 - putative RNA 2'-O-ribose methyltransferase (RefSeq), at 3,266,754 to 3,267,845
_2761
Shew_2762: Shew_2762 - hypothetical protein (RefSeq), at 3,267,937 to 3,268,326
_2762
Shew_2763: Shew_2763 - dienelactone hydrolase (RefSeq), at 3,268,319 to 3,269,041
_2763
Shew_2764: Shew_2764 - DNA-binding transcriptional activator GcvA (RefSeq), at 3,269,102 to 3,270,013
_2764
Position (kb)
3268
3269
3270 Strain fitness (log2 ratio)
-2
-1
0
1 at 3267.402 kb on + strand, within Shew_2761 at 3267.567 kb on - strand, within Shew_2761 at 3267.709 kb on - strand, within Shew_2761 at 3267.900 kb on - strand at 3268.332 kb on - strand at 3268.392 kb on - strand, within Shew_2763 at 3268.671 kb on + strand, within Shew_2763 at 3268.938 kb on + strand, within Shew_2763 at 3269.551 kb on + strand, within Shew_2764 at 3269.551 kb on + strand, within Shew_2764
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,267,402 + Shew_2761 0.59 +0.5 3,267,567 - Shew_2761 0.74 -0.2 3,267,709 - Shew_2761 0.87 -0.5 3,267,900 - +0.4 3,268,332 - -0.2 3,268,392 - Shew_2763 0.10 -0.6 3,268,671 + Shew_2763 0.49 -1.0 3,268,938 + Shew_2763 0.86 +0.1 3,269,551 + Shew_2764 0.49 -2.5 3,269,551 + Shew_2764 0.49 -0.5
Or see this region's nucleotide sequence