Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspB and Shew_2496 are separated by 20 nucleotides Shew_2496 and Shew_2497 are separated by 177 nucleotides Shew_2497 and Shew_2498 are separated by 87 nucleotides
Shew_2495: pspB - phage shock protein B (RefSeq), at 2,934,975 to 2,935,214
pspB
Shew_2496: Shew_2496 - phage shock protein A, PspA (RefSeq), at 2,935,235 to 2,935,918
_2496
Shew_2497: Shew_2497 - sigma-54 dependent trancsriptional regulator (RefSeq), at 2,936,096 to 2,937,166
_2497
Shew_2498: Shew_2498 - extracellular solute-binding protein (RefSeq), at 2,937,254 to 2,938,897
_2498
Position (kb)
2936
2937
2938 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 2935.299 kb on - strand at 2935.360 kb on + strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.682 kb on - strand, within Shew_2496 at 2935.953 kb on + strand at 2936.122 kb on + strand at 2936.130 kb on - strand at 2936.130 kb on - strand at 2936.142 kb on - strand at 2936.142 kb on - strand at 2936.249 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.444 kb on - strand, within Shew_2497 at 2936.517 kb on + strand, within Shew_2497 at 2936.810 kb on + strand, within Shew_2497 at 2936.961 kb on + strand, within Shew_2497 at 2937.207 kb on - strand at 2937.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,935,299 - +0.1 2,935,360 + Shew_2496 0.18 +2.1 2,935,611 - Shew_2496 0.55 +5.6 2,935,611 - Shew_2496 0.55 +4.9 2,935,682 - Shew_2496 0.65 +5.8 2,935,953 + +5.6 2,936,122 + +0.3 2,936,130 - -0.1 2,936,130 - +0.4 2,936,142 - +0.4 2,936,142 - -0.8 2,936,249 + Shew_2497 0.14 -0.5 2,936,436 + Shew_2497 0.32 -0.0 2,936,436 + Shew_2497 0.32 +0.2 2,936,444 - Shew_2497 0.32 -1.2 2,936,517 + Shew_2497 0.39 -0.6 2,936,810 + Shew_2497 0.67 -0.4 2,936,961 + Shew_2497 0.81 -0.4 2,937,207 - +5.6 2,937,282 - +4.9
Or see this region's nucleotide sequence