Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1725 and Shew_1726 are separated by 161 nucleotides Shew_1726 and Shew_1727 overlap by 4 nucleotides Shew_1727 and Shew_1728 are separated by 14 nucleotides Shew_1728 and Shew_1729 are separated by 2 nucleotides
Shew_1725: Shew_1725 - curlin-associated protein (RefSeq), at 1,979,156 to 1,979,707
_1725
Shew_1726: Shew_1726 - LuxR family transcriptional regulator (RefSeq), at 1,979,869 to 1,980,495
_1726
Shew_1727: Shew_1727 - hypothetical protein (RefSeq), at 1,980,492 to 1,980,959
_1727
Shew_1728: Shew_1728 - curli production assembly/transport component CsgF precursor (RefSeq), at 1,980,974 to 1,981,393
_1728
Shew_1729: Shew_1729 - curli production assembly/transport component CsgG (RefSeq), at 1,981,396 to 1,982,244
_1729
Position (kb)
1980
1981 Strain fitness (log2 ratio)
-2
-1
0
1 at 1979.731 kb on - strand at 1979.811 kb on + strand at 1979.939 kb on + strand, within Shew_1726 at 1979.992 kb on - strand, within Shew_1726 at 1980.119 kb on + strand, within Shew_1726 at 1980.347 kb on + strand, within Shew_1726 at 1980.411 kb on - strand, within Shew_1726 at 1980.864 kb on + strand, within Shew_1727 at 1981.109 kb on - strand, within Shew_1728 at 1981.154 kb on + strand, within Shew_1728 at 1981.632 kb on + strand, within Shew_1729 at 1981.958 kb on + strand, within Shew_1729
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,979,731 - +0.5 1,979,811 + -0.1 1,979,939 + Shew_1726 0.11 -0.9 1,979,992 - Shew_1726 0.20 -1.9 1,980,119 + Shew_1726 0.40 -0.2 1,980,347 + Shew_1726 0.76 -0.5 1,980,411 - Shew_1726 0.86 +0.1 1,980,864 + Shew_1727 0.79 -1.1 1,981,109 - Shew_1728 0.32 -0.2 1,981,154 + Shew_1728 0.43 -0.0 1,981,632 + Shew_1729 0.28 -0.1 1,981,958 + Shew_1729 0.66 -0.2
Or see this region's nucleotide sequence