Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1258 and Shew_1259 are separated by 284 nucleotides Shew_1259 and Shew_1260 are separated by 370 nucleotides Shew_1260 and Shew_1261 are separated by 5 nucleotides
Shew_1258: Shew_1258 - phage transcriptional regulator, AlpA (RefSeq), at 1,454,598 to 1,454,783
_1258
Shew_1259: Shew_1259 - phage transcriptional regulator, AlpA (RefSeq), at 1,455,068 to 1,455,226
_1259
Shew_1260: Shew_1260 - DNA methylase N-4/N-6 domain-containing protein (RefSeq), at 1,455,597 to 1,457,756
_1260
Shew_1261: Shew_1261 - type III restriction enzyme, res subunit (RefSeq), at 1,457,762 to 1,460,347
_1261
Position (kb)
1455
1456
1457
1458 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1454.673 kb on - strand, within Shew_1258 at 1454.958 kb on - strand at 1455.085 kb on + strand, within Shew_1259 at 1455.118 kb on + strand, within Shew_1259 at 1455.383 kb on + strand at 1455.391 kb on - strand at 1455.565 kb on + strand at 1455.581 kb on - strand at 1455.626 kb on + strand at 1455.733 kb on + strand at 1455.830 kb on + strand, within Shew_1260 at 1456.060 kb on - strand, within Shew_1260 at 1456.100 kb on + strand, within Shew_1260 at 1456.734 kb on + strand, within Shew_1260 at 1456.837 kb on + strand, within Shew_1260 at 1457.340 kb on - strand, within Shew_1260 at 1457.692 kb on + strand at 1457.884 kb on + strand at 1458.270 kb on + strand, within Shew_1261
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,454,673 - Shew_1258 0.40 +2.9 1,454,958 - +0.4 1,455,085 + Shew_1259 0.11 -0.2 1,455,118 + Shew_1259 0.31 -0.1 1,455,383 + +0.3 1,455,391 - -0.1 1,455,565 + -0.7 1,455,581 - -0.1 1,455,626 + -1.0 1,455,733 + -0.7 1,455,830 + Shew_1260 0.11 -0.6 1,456,060 - Shew_1260 0.21 -1.0 1,456,100 + Shew_1260 0.23 -0.7 1,456,734 + Shew_1260 0.53 -1.5 1,456,837 + Shew_1260 0.57 -0.5 1,457,340 - Shew_1260 0.81 -0.4 1,457,692 + -0.6 1,457,884 + +0.2 1,458,270 + Shew_1261 0.20 -0.0
Or see this region's nucleotide sequence