Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1220 and Shew_1221 are separated by 8 nucleotides Shew_1221 and Shew_1222 are separated by 363 nucleotides Shew_1222 and Shew_1223 are separated by 29 nucleotides Shew_1223 and Shew_1224 are separated by 132 nucleotides
Shew_1220: Shew_1220 - pyruvate carboxylase subunit B (RefSeq), at 1,417,554 to 1,419,359
_1220
Shew_1221: Shew_1221 - sodium ion-translocating decarboxylase, beta subunit (RefSeq), at 1,419,368 to 1,420,501
_1221
Shew_1222: Shew_1222 - hypothetical protein (RefSeq), at 1,420,865 to 1,421,050
_1222
Shew_1223: Shew_1223 - hypothetical protein (RefSeq), at 1,421,080 to 1,421,265
_1223
Shew_1224: Shew_1224 - sodium:dicarboxylate symporter (RefSeq), at 1,421,398 to 1,422,648
_1224
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-1
0
1
2 at 1418.535 kb on - strand, within Shew_1220 at 1418.535 kb on - strand, within Shew_1220 at 1418.612 kb on + strand, within Shew_1220 at 1418.620 kb on - strand, within Shew_1220 at 1418.984 kb on - strand, within Shew_1220 at 1419.711 kb on - strand, within Shew_1221 at 1419.937 kb on + strand, within Shew_1221 at 1419.955 kb on + strand, within Shew_1221 at 1419.955 kb on + strand, within Shew_1221 at 1419.980 kb on - strand, within Shew_1221 at 1420.573 kb on + strand at 1420.581 kb on - strand at 1420.690 kb on - strand at 1420.975 kb on - strand, within Shew_1222 at 1421.139 kb on + strand, within Shew_1223
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,418,535 - Shew_1220 0.54 -0.4 1,418,535 - Shew_1220 0.54 +0.2 1,418,612 + Shew_1220 0.59 +0.1 1,418,620 - Shew_1220 0.59 -1.3 1,418,984 - Shew_1220 0.79 +0.3 1,419,711 - Shew_1221 0.30 +0.2 1,419,937 + Shew_1221 0.50 +0.8 1,419,955 + Shew_1221 0.52 +1.8 1,419,955 + Shew_1221 0.52 -0.2 1,419,980 - Shew_1221 0.54 +0.2 1,420,573 + +0.2 1,420,581 - +0.2 1,420,690 - +0.3 1,420,975 - Shew_1222 0.59 +0.3 1,421,139 + Shew_1223 0.32 +0.5
Or see this region's nucleotide sequence