Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1073 and Shew_1074 are separated by 262 nucleotides Shew_1074 and Shew_1075 are separated by 169 nucleotides Shew_1075 and Shew_1076 are separated by 152 nucleotides
Shew_1073: Shew_1073 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 1,237,259 to 1,237,633
_1073
Shew_1074: Shew_1074 - cytochrome B561 (RefSeq), at 1,237,896 to 1,238,591
_1074
Shew_1075: Shew_1075 - cytochrome c, class II (RefSeq), at 1,238,761 to 1,239,222
_1075
Shew_1076: Shew_1076 - Trp operon repressor (RefSeq), at 1,239,375 to 1,239,728
_1076
Position (kb)
1237
1238
1239 Strain fitness (log2 ratio)
-1
0
1
2 at 1236.989 kb on - strand at 1237.116 kb on + strand at 1237.116 kb on + strand at 1237.224 kb on - strand at 1237.537 kb on + strand, within Shew_1073 at 1237.818 kb on + strand at 1237.826 kb on - strand at 1237.900 kb on + strand at 1237.908 kb on - strand at 1237.993 kb on - strand, within Shew_1074 at 1238.008 kb on - strand, within Shew_1074 at 1238.260 kb on + strand, within Shew_1074 at 1238.268 kb on - strand, within Shew_1074 at 1238.345 kb on - strand, within Shew_1074 at 1239.549 kb on - strand, within Shew_1076 at 1239.580 kb on - strand, within Shew_1076
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,236,989 - +0.1 1,237,116 + +0.4 1,237,116 + +1.7 1,237,224 - -0.4 1,237,537 + Shew_1073 0.74 +1.0 1,237,818 + +0.3 1,237,826 - +0.2 1,237,900 + -0.0 1,237,908 - +1.6 1,237,993 - Shew_1074 0.14 +1.7 1,238,008 - Shew_1074 0.16 +1.6 1,238,260 + Shew_1074 0.52 +1.5 1,238,268 - Shew_1074 0.53 +2.1 1,238,345 - Shew_1074 0.65 +1.3 1,239,549 - Shew_1076 0.49 +0.3 1,239,580 - Shew_1076 0.58 +0.4
Or see this region's nucleotide sequence