Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0280 and Shew_0281 are separated by 193 nucleotides Shew_0281 and Shew_0282 are separated by 24 nucleotides
Shew_0280: Shew_0280 - GntR family transcriptional regulator (RefSeq), at 297,449 to 298,150
_0280
Shew_0281: Shew_0281 - phospholipid/glycerol acyltransferase (RefSeq), at 298,344 to 299,291
_0281
Shew_0282: Shew_0282 - phospholipid/glycerol acyltransferase (RefSeq), at 299,316 to 300,200
_0282
Position (kb) 298
299
300 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 297.446 kb on + strand at 297.454 kb on - strand at 297.752 kb on - strand, within Shew_0280 at 297.875 kb on + strand, within Shew_0280 at 297.893 kb on - strand, within Shew_0280 at 298.049 kb on - strand, within Shew_0280 at 298.618 kb on - strand, within Shew_0281 at 298.697 kb on + strand, within Shew_0281 at 298.767 kb on + strand, within Shew_0281 at 298.777 kb on + strand, within Shew_0281 at 298.777 kb on + strand, within Shew_0281 at 298.855 kb on + strand, within Shew_0281 at 298.978 kb on - strand, within Shew_0281 at 299.205 kb on + strand at 299.260 kb on + strand at 299.312 kb on - strand at 299.604 kb on - strand, within Shew_0282 at 299.796 kb on + strand, within Shew_0282 at 299.825 kb on - strand, within Shew_0282 at 300.258 kb on + strand at 300.289 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 297,446 + -1.6 297,454 - +0.1 297,752 - Shew_0280 0.43 +0.2 297,875 + Shew_0280 0.61 -0.0 297,893 - Shew_0280 0.63 -0.0 298,049 - Shew_0280 0.85 -0.7 298,618 - Shew_0281 0.29 -3.5 298,697 + Shew_0281 0.37 -1.9 298,767 + Shew_0281 0.45 -1.9 298,777 + Shew_0281 0.46 -2.0 298,777 + Shew_0281 0.46 -0.6 298,855 + Shew_0281 0.54 -2.3 298,978 - Shew_0281 0.67 -2.1 299,205 + -2.1 299,260 + -2.0 299,312 - -0.5 299,604 - Shew_0282 0.33 -1.3 299,796 + Shew_0282 0.54 -1.3 299,825 - Shew_0282 0.58 -0.8 300,258 + -0.4 300,289 - +0.6
Or see this region's nucleotide sequence