Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0080 and Shew_0081 are separated by 6 nucleotides Shew_0081 and Shew_0082 are separated by 52 nucleotides Shew_0082 and Shew_0083 are separated by 134 nucleotides Shew_0083 and Shew_0084 are separated by 81 nucleotides
Shew_0080: Shew_0080 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 93,574 to 94,866
_0080
Shew_0081: Shew_0081 - two component transcriptional regulator (RefSeq), at 94,873 to 95,607
_0081
Shew_0082: Shew_0082 - hypothetical protein (RefSeq), at 95,660 to 96,109
_0082
Shew_0083: Shew_0083 - MOSC domain-containing protein (RefSeq), at 96,244 to 96,936
_0083
Shew_0084: Shew_0084 - hypothetical protein (RefSeq), at 97,018 to 97,338
_0084
Position (kb)
95
96
97 Strain fitness (log2 ratio)
-1
0
1 at 94.694 kb on + strand, within Shew_0080 at 94.785 kb on + strand at 94.802 kb on - strand at 94.843 kb on - strand at 95.002 kb on + strand, within Shew_0081 at 95.439 kb on + strand, within Shew_0081 at 95.887 kb on + strand, within Shew_0082 at 95.890 kb on - strand, within Shew_0082 at 95.948 kb on - strand, within Shew_0082 at 96.087 kb on - strand at 96.187 kb on - strand at 96.371 kb on - strand, within Shew_0083 at 96.658 kb on + strand, within Shew_0083 at 96.747 kb on - strand, within Shew_0083 at 96.763 kb on - strand, within Shew_0083 at 96.805 kb on - strand, within Shew_0083
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 94,694 + Shew_0080 0.87 -0.6 94,785 + +0.6 94,802 - +0.8 94,843 - +0.1 95,002 + Shew_0081 0.18 -0.3 95,439 + Shew_0081 0.77 +0.1 95,887 + Shew_0082 0.50 -0.6 95,890 - Shew_0082 0.51 -0.9 95,948 - Shew_0082 0.64 +0.1 96,087 - +0.2 96,187 - -1.6 96,371 - Shew_0083 0.18 +1.0 96,658 + Shew_0083 0.60 +0.1 96,747 - Shew_0083 0.73 -0.1 96,763 - Shew_0083 0.75 -0.1 96,805 - Shew_0083 0.81 +0.4
Or see this region's nucleotide sequence