Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_959 and Pf6N2E2_960 are separated by 82 nucleotides Pf6N2E2_960 and Pf6N2E2_961 overlap by 4 nucleotides Pf6N2E2_961 and Pf6N2E2_962 overlap by 4 nucleotides
Pf6N2E2_959 - putative membrane protein, at 1,130,027 to 1,130,761
_959
Pf6N2E2_960 - Type IV secretory pathway, VirD4 components, at 1,130,844 to 1,132,964
_960
Pf6N2E2_961 - FIG00954712: hypothetical protein, at 1,132,961 to 1,133,476
_961
Pf6N2E2_962 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,133,473 to 1,133,988
_962
Position (kb)
1130
1131
1132
1133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1130.031 kb on - strand at 1130.031 kb on - strand at 1130.137 kb on - strand, within Pf6N2E2_959 at 1130.337 kb on + strand, within Pf6N2E2_959 at 1130.530 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.582 kb on - strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.615 kb on + strand, within Pf6N2E2_959 at 1130.811 kb on + strand at 1130.873 kb on + strand at 1130.873 kb on + strand at 1131.275 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.309 kb on + strand, within Pf6N2E2_960 at 1131.343 kb on + strand, within Pf6N2E2_960 at 1131.344 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.503 kb on - strand, within Pf6N2E2_960 at 1131.596 kb on - strand, within Pf6N2E2_960 at 1131.665 kb on - strand, within Pf6N2E2_960 at 1131.678 kb on + strand, within Pf6N2E2_960 at 1131.679 kb on - strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.748 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960 at 1131.752 kb on + strand, within Pf6N2E2_960 at 1131.802 kb on - strand, within Pf6N2E2_960 at 1131.860 kb on + strand, within Pf6N2E2_960 at 1131.956 kb on - strand, within Pf6N2E2_960 at 1132.049 kb on + strand, within Pf6N2E2_960 at 1132.050 kb on - strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.118 kb on + strand, within Pf6N2E2_960 at 1132.119 kb on - strand, within Pf6N2E2_960 at 1132.165 kb on + strand, within Pf6N2E2_960 at 1132.165 kb on + strand, within Pf6N2E2_960 at 1132.191 kb on + strand, within Pf6N2E2_960 at 1132.192 kb on - strand, within Pf6N2E2_960 at 1132.204 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.243 kb on + strand, within Pf6N2E2_960 at 1132.244 kb on - strand, within Pf6N2E2_960 at 1132.244 kb on - strand, within Pf6N2E2_960 at 1132.330 kb on + strand, within Pf6N2E2_960 at 1132.330 kb on + strand, within Pf6N2E2_960 at 1132.412 kb on + strand, within Pf6N2E2_960 at 1132.412 kb on + strand, within Pf6N2E2_960 at 1132.413 kb on - strand, within Pf6N2E2_960 at 1132.461 kb on + strand, within Pf6N2E2_960 at 1132.577 kb on + strand, within Pf6N2E2_960 at 1132.577 kb on + strand, within Pf6N2E2_960 at 1132.578 kb on - strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.579 kb on + strand, within Pf6N2E2_960 at 1132.600 kb on + strand, within Pf6N2E2_960 at 1132.601 kb on - strand, within Pf6N2E2_960 at 1132.804 kb on + strand at 1132.862 kb on - strand at 1132.862 kb on - strand at 1132.897 kb on - strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.903 kb on + strand at 1132.904 kb on - strand at 1133.189 kb on + strand, within Pf6N2E2_961 at 1133.207 kb on + strand, within Pf6N2E2_961 at 1133.345 kb on + strand, within Pf6N2E2_961 at 1133.458 kb on + strand at 1133.459 kb on - strand at 1133.465 kb on + strand at 1133.812 kb on + strand, within Pf6N2E2_962 at 1133.812 kb on + strand, within Pf6N2E2_962 at 1133.932 kb on + strand, within Pf6N2E2_962
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 1,130,031 - -3.2 1,130,031 - +0.3 1,130,137 - Pf6N2E2_959 0.15 -1.9 1,130,337 + Pf6N2E2_959 0.42 +0.3 1,130,530 - Pf6N2E2_959 0.68 -1.3 1,130,582 - Pf6N2E2_959 0.76 -1.3 1,130,582 - Pf6N2E2_959 0.76 -0.5 1,130,615 + Pf6N2E2_959 0.80 -2.8 1,130,615 + Pf6N2E2_959 0.80 +1.1 1,130,811 + +0.2 1,130,873 + +2.5 1,130,873 + +2.2 1,131,275 + Pf6N2E2_960 0.20 +2.5 1,131,309 + Pf6N2E2_960 0.22 +1.4 1,131,309 + Pf6N2E2_960 0.22 +2.0 1,131,343 + Pf6N2E2_960 0.24 +0.3 1,131,344 - Pf6N2E2_960 0.24 +1.5 1,131,503 - Pf6N2E2_960 0.31 -1.5 1,131,503 - Pf6N2E2_960 0.31 +1.5 1,131,596 - Pf6N2E2_960 0.35 -0.9 1,131,665 - Pf6N2E2_960 0.39 -0.5 1,131,678 + Pf6N2E2_960 0.39 +0.5 1,131,679 - Pf6N2E2_960 0.39 -2.5 1,131,748 + Pf6N2E2_960 0.43 -1.3 1,131,748 + Pf6N2E2_960 0.43 +0.3 1,131,748 + Pf6N2E2_960 0.43 +0.5 1,131,752 + Pf6N2E2_960 0.43 -0.3 1,131,752 + Pf6N2E2_960 0.43 -3.0 1,131,802 - Pf6N2E2_960 0.45 -0.5 1,131,860 + Pf6N2E2_960 0.48 -0.7 1,131,956 - Pf6N2E2_960 0.52 +1.5 1,132,049 + Pf6N2E2_960 0.57 +2.1 1,132,050 - Pf6N2E2_960 0.57 -0.5 1,132,118 + Pf6N2E2_960 0.60 +1.7 1,132,118 + Pf6N2E2_960 0.60 +0.8 1,132,118 + Pf6N2E2_960 0.60 -2.3 1,132,118 + Pf6N2E2_960 0.60 -1.3 1,132,119 - Pf6N2E2_960 0.60 -0.2 1,132,165 + Pf6N2E2_960 0.62 -1.7 1,132,165 + Pf6N2E2_960 0.62 -1.5 1,132,191 + Pf6N2E2_960 0.64 +1.2 1,132,192 - Pf6N2E2_960 0.64 +2.1 1,132,204 + Pf6N2E2_960 0.64 +1.4 1,132,243 + Pf6N2E2_960 0.66 +1.3 1,132,243 + Pf6N2E2_960 0.66 +1.5 1,132,243 + Pf6N2E2_960 0.66 +1.1 1,132,244 - Pf6N2E2_960 0.66 -0.1 1,132,244 - Pf6N2E2_960 0.66 -0.9 1,132,330 + Pf6N2E2_960 0.70 +2.2 1,132,330 + Pf6N2E2_960 0.70 -0.0 1,132,412 + Pf6N2E2_960 0.74 -0.4 1,132,412 + Pf6N2E2_960 0.74 +0.5 1,132,413 - Pf6N2E2_960 0.74 +0.5 1,132,461 + Pf6N2E2_960 0.76 -0.4 1,132,577 + Pf6N2E2_960 0.82 +1.3 1,132,577 + Pf6N2E2_960 0.82 +0.7 1,132,578 - Pf6N2E2_960 0.82 -1.4 1,132,579 + Pf6N2E2_960 0.82 +0.4 1,132,579 + Pf6N2E2_960 0.82 +1.8 1,132,579 + Pf6N2E2_960 0.82 +2.0 1,132,579 + Pf6N2E2_960 0.82 -0.7 1,132,579 + Pf6N2E2_960 0.82 +0.9 1,132,579 + Pf6N2E2_960 0.82 -1.6 1,132,600 + Pf6N2E2_960 0.83 -0.7 1,132,601 - Pf6N2E2_960 0.83 -2.5 1,132,804 + +0.1 1,132,862 - +2.2 1,132,862 - -3.2 1,132,897 - +1.8 1,132,903 + -1.5 1,132,903 + -0.6 1,132,903 + +1.0 1,132,903 + -0.5 1,132,904 - +2.9 1,133,189 + Pf6N2E2_961 0.44 -0.4 1,133,207 + Pf6N2E2_961 0.48 +0.5 1,133,345 + Pf6N2E2_961 0.74 +1.7 1,133,458 + -3.4 1,133,459 - -2.8 1,133,465 + +0.1 1,133,812 + Pf6N2E2_962 0.66 -1.6 1,133,812 + Pf6N2E2_962 0.66 -1.3 1,133,932 + Pf6N2E2_962 0.89 +1.0
Or see this region's nucleotide sequence