Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_873 and Pf6N2E2_874 are separated by 115 nucleotides Pf6N2E2_874 and Pf6N2E2_875 are separated by 60 nucleotides Pf6N2E2_875 and Pf6N2E2_876 overlap by 4 nucleotides
Pf6N2E2_873 - Methyl-accepting chemotaxis protein, at 1,023,063 to 1,024,352
_873
Pf6N2E2_874 - Predicted cobalt transporter CbtC, at 1,024,468 to 1,025,145
_874
Pf6N2E2_875 - putative metal chaperone, involved in Fe-nitrile hydratase activation, GTPase of COG0523 family, at 1,025,206 to 1,025,646
_875
Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296
_876
Position (kb)
1024
1025
1026 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873 at 1023.969 kb on + strand, within Pf6N2E2_873 at 1023.970 kb on - strand, within Pf6N2E2_873 at 1023.973 kb on + strand, within Pf6N2E2_873 at 1023.973 kb on + strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand at 1024.020 kb on + strand, within Pf6N2E2_873 at 1024.021 kb on - strand, within Pf6N2E2_873 at 1024.021 kb on - strand, within Pf6N2E2_873 at 1024.275 kb on + strand at 1024.275 kb on + strand at 1024.276 kb on - strand at 1024.276 kb on - strand at 1024.337 kb on + strand at 1024.338 kb on - strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.482 kb on - strand at 1024.586 kb on + strand, within Pf6N2E2_874 at 1024.586 kb on + strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.643 kb on + strand, within Pf6N2E2_874 at 1024.644 kb on - strand, within Pf6N2E2_874 at 1024.713 kb on + strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.814 kb on + strand, within Pf6N2E2_874 at 1024.815 kb on - strand, within Pf6N2E2_874 at 1024.963 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1025.069 kb on + strand, within Pf6N2E2_874 at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.097 kb on - strand at 1025.097 kb on - strand at 1025.130 kb on + strand at 1025.322 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.491 kb on + strand, within Pf6N2E2_875 at 1025.492 kb on - strand, within Pf6N2E2_875 at 1025.621 kb on + strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.647 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.670 kb on + strand at 1025.670 kb on + strand at 1025.737 kb on + strand, within Pf6N2E2_876 at 1025.761 kb on - strand, within Pf6N2E2_876 at 1025.976 kb on + strand, within Pf6N2E2_876 at 1026.092 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.109 kb on + strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 1,023,656 + Pf6N2E2_873 0.46 -1.9 1,023,656 + Pf6N2E2_873 0.46 -0.7 1,023,657 - Pf6N2E2_873 0.46 +1.3 1,023,657 - Pf6N2E2_873 0.46 +0.5 1,023,657 - Pf6N2E2_873 0.46 +0.6 1,023,657 - Pf6N2E2_873 0.46 -0.4 1,023,835 + Pf6N2E2_873 0.60 -0.7 1,023,835 + Pf6N2E2_873 0.60 +2.3 1,023,969 + Pf6N2E2_873 0.70 -1.0 1,023,970 - Pf6N2E2_873 0.70 +0.6 1,023,973 + Pf6N2E2_873 0.71 +1.0 1,023,973 + Pf6N2E2_873 0.71 -2.1 1,023,974 - Pf6N2E2_873 0.71 +1.2 1,023,974 - Pf6N2E2_873 0.71 +0.6 1,023,974 - Pf6N2E2_873 0.71 -1.9 1,023,974 - Pf6N2E2_873 0.71 +0.7 1,023,974 - Pf6N2E2_873 0.71 -1.7 1,023,974 - Pf6N2E2_873 0.71 -0.0 1,023,974 - +0.6 1,024,020 + Pf6N2E2_873 0.74 -1.2 1,024,021 - Pf6N2E2_873 0.74 +1.7 1,024,021 - Pf6N2E2_873 0.74 -0.3 1,024,275 + +0.7 1,024,275 + +1.4 1,024,276 - +3.2 1,024,276 - -1.3 1,024,337 + -2.3 1,024,338 - -2.3 1,024,357 + -0.3 1,024,357 + +2.1 1,024,357 + +0.1 1,024,357 + +1.0 1,024,482 - +0.8 1,024,586 + Pf6N2E2_874 0.17 +0.3 1,024,586 + Pf6N2E2_874 0.17 -1.4 1,024,587 - Pf6N2E2_874 0.18 +1.2 1,024,587 - Pf6N2E2_874 0.18 -0.2 1,024,587 - Pf6N2E2_874 0.18 +1.3 1,024,587 - Pf6N2E2_874 0.18 -0.3 1,024,587 - Pf6N2E2_874 0.18 +0.2 1,024,643 + Pf6N2E2_874 0.26 -2.2 1,024,644 - Pf6N2E2_874 0.26 -1.9 1,024,713 + Pf6N2E2_874 0.36 -0.3 1,024,714 - Pf6N2E2_874 0.36 -1.3 1,024,714 - Pf6N2E2_874 0.36 -0.3 1,024,715 + Pf6N2E2_874 0.36 +1.3 1,024,715 + Pf6N2E2_874 0.36 -0.4 1,024,715 + Pf6N2E2_874 0.36 +0.6 1,024,716 - Pf6N2E2_874 0.37 +0.6 1,024,716 - Pf6N2E2_874 0.37 -0.1 1,024,716 - Pf6N2E2_874 0.37 +0.4 1,024,730 + Pf6N2E2_874 0.39 -3.4 1,024,730 + Pf6N2E2_874 0.39 -0.7 1,024,730 + Pf6N2E2_874 0.39 +0.6 1,024,730 + Pf6N2E2_874 0.39 +1.7 1,024,730 + Pf6N2E2_874 0.39 +1.2 1,024,730 + Pf6N2E2_874 0.39 -0.2 1,024,731 - Pf6N2E2_874 0.39 -0.0 1,024,731 - Pf6N2E2_874 0.39 +1.0 1,024,814 + Pf6N2E2_874 0.51 +0.5 1,024,815 - Pf6N2E2_874 0.51 +0.5 1,024,963 + Pf6N2E2_874 0.73 +0.5 1,024,984 + Pf6N2E2_874 0.76 -0.1 1,024,984 + Pf6N2E2_874 0.76 -1.1 1,024,985 - Pf6N2E2_874 0.76 -1.2 1,024,985 - Pf6N2E2_874 0.76 +1.5 1,025,069 + Pf6N2E2_874 0.89 -2.0 1,025,096 + +1.3 1,025,096 + -0.2 1,025,096 + +1.6 1,025,096 + -1.8 1,025,096 + +0.2 1,025,096 + +0.6 1,025,097 - +0.9 1,025,097 - +0.8 1,025,130 + -1.6 1,025,322 + Pf6N2E2_875 0.26 -0.7 1,025,330 + Pf6N2E2_875 0.28 +1.6 1,025,330 + Pf6N2E2_875 0.28 -0.8 1,025,331 - Pf6N2E2_875 0.28 +1.8 1,025,331 - Pf6N2E2_875 0.28 -1.4 1,025,331 - Pf6N2E2_875 0.28 +1.9 1,025,331 - Pf6N2E2_875 0.28 +1.9 1,025,365 - Pf6N2E2_875 0.36 +2.2 1,025,365 - Pf6N2E2_875 0.36 -0.0 1,025,432 + Pf6N2E2_875 0.51 -0.1 1,025,432 + Pf6N2E2_875 0.51 +0.8 1,025,432 + Pf6N2E2_875 0.51 +0.2 1,025,432 + Pf6N2E2_875 0.51 +1.5 1,025,432 + Pf6N2E2_875 0.51 -1.2 1,025,433 - Pf6N2E2_875 0.51 -1.0 1,025,433 - Pf6N2E2_875 0.51 -1.2 1,025,433 - Pf6N2E2_875 0.51 +0.6 1,025,433 - Pf6N2E2_875 0.51 -0.7 1,025,433 - Pf6N2E2_875 0.51 -0.2 1,025,433 - Pf6N2E2_875 0.51 -1.3 1,025,433 - Pf6N2E2_875 0.51 +1.4 1,025,433 - Pf6N2E2_875 0.51 +1.7 1,025,491 + Pf6N2E2_875 0.65 +1.9 1,025,492 - Pf6N2E2_875 0.65 +1.2 1,025,621 + +1.1 1,025,622 - -2.0 1,025,622 - +1.3 1,025,622 - -0.8 1,025,622 - -0.7 1,025,647 - -1.2 1,025,660 - -1.3 1,025,660 - +0.1 1,025,660 - -1.1 1,025,670 + -1.7 1,025,670 + -1.0 1,025,737 + Pf6N2E2_876 0.14 +0.5 1,025,761 - Pf6N2E2_876 0.18 +0.3 1,025,976 + Pf6N2E2_876 0.51 -1.9 1,026,092 - Pf6N2E2_876 0.69 +0.2 1,026,095 - Pf6N2E2_876 0.69 -0.2 1,026,095 - Pf6N2E2_876 0.69 +1.2 1,026,109 + Pf6N2E2_876 0.71 +0.3 1,026,110 - Pf6N2E2_876 0.71 -2.1 1,026,110 - Pf6N2E2_876 0.71 -0.7 1,026,110 - Pf6N2E2_876 0.71 +0.6
Or see this region's nucleotide sequence