Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_81 and Pf6N2E2_82 are separated by 249 nucleotides Pf6N2E2_82 and Pf6N2E2_83 are separated by 361 nucleotides
Pf6N2E2_81 - hypothetical protein, at 126,543 to 126,749
_81
Pf6N2E2_82 - hypothetical protein, at 126,999 to 127,151
_82
Pf6N2E2_83 - transcriptional regulator, AraC family, at 127,513 to 128,382
_83
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 126.553 kb on - strand at 126.569 kb on - strand, within Pf6N2E2_81 at 126.569 kb on - strand, within Pf6N2E2_81 at 126.693 kb on - strand, within Pf6N2E2_81 at 126.747 kb on + strand at 126.748 kb on - strand at 126.748 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.881 kb on - strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.988 kb on + strand at 126.988 kb on + strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 127.050 kb on - strand, within Pf6N2E2_82 at 127.093 kb on + strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.226 kb on + strand at 127.302 kb on - strand at 127.349 kb on - strand at 127.397 kb on + strand at 127.398 kb on - strand at 127.398 kb on - strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.568 kb on + strand at 127.569 kb on - strand at 127.580 kb on + strand at 127.580 kb on + strand at 127.581 kb on - strand at 127.581 kb on - strand at 127.649 kb on + strand, within Pf6N2E2_83 at 127.890 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 128.140 kb on + strand, within Pf6N2E2_83 at 128.185 kb on + strand, within Pf6N2E2_83 at 128.204 kb on + strand, within Pf6N2E2_83 at 128.204 kb on + strand, within Pf6N2E2_83 at 128.204 kb on + strand, within Pf6N2E2_83 at 128.205 kb on - strand, within Pf6N2E2_83 at 128.205 kb on - strand, within Pf6N2E2_83 at 128.205 kb on - strand, within Pf6N2E2_83 at 128.205 kb on - strand, within Pf6N2E2_83 at 128.286 kb on + strand, within Pf6N2E2_83 at 128.286 kb on + strand, within Pf6N2E2_83 at 128.287 kb on - strand, within Pf6N2E2_83 at 128.287 kb on - strand, within Pf6N2E2_83 at 128.287 kb on - strand, within Pf6N2E2_83 at 128.287 kb on - strand, within Pf6N2E2_83 at 128.319 kb on + strand at 128.320 kb on - strand at 128.320 kb on - strand at 128.324 kb on + strand at 128.325 kb on - strand at 128.404 kb on - strand at 128.404 kb on - strand at 128.404 kb on - strand at 128.657 kb on + strand at 128.658 kb on - strand at 128.658 kb on - strand at 128.672 kb on + strand at 128.673 kb on - strand at 128.673 kb on - strand at 128.679 kb on + strand at 128.680 kb on - strand at 128.696 kb on + strand at 128.697 kb on - strand at 128.697 kb on - strand at 128.698 kb on + strand at 128.698 kb on + strand at 128.699 kb on - strand at 128.699 kb on - strand at 128.712 kb on + strand at 128.764 kb on - strand at 128.764 kb on - strand at 128.764 kb on - strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.765 kb on + strand at 128.766 kb on - strand at 128.766 kb on - strand at 128.766 kb on - strand at 128.847 kb on + strand at 128.847 kb on + strand at 128.848 kb on - strand at 128.848 kb on - strand at 128.945 kb on + strand at 129.015 kb on - strand at 129.334 kb on + strand at 129.358 kb on + strand at 129.358 kb on + strand at 129.358 kb on + strand at 129.359 kb on - strand at 129.359 kb on - strand at 129.363 kb on - strand at 129.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 126,553 - +1.7 126,569 - Pf6N2E2_81 0.13 +3.4 126,569 - Pf6N2E2_81 0.13 -0.4 126,693 - Pf6N2E2_81 0.72 -1.1 126,747 + +0.6 126,748 - +2.8 126,748 - +2.3 126,815 - -1.4 126,815 - -0.5 126,815 - -1.1 126,881 - -2.5 126,964 + +0.3 126,964 + -2.0 126,964 + +2.8 126,964 + -0.9 126,965 - +0.1 126,965 - -3.1 126,965 - -0.0 126,965 - -0.8 126,965 - +1.8 126,965 - +0.7 126,965 - -1.9 126,965 - +1.4 126,965 - +0.9 126,988 + -0.5 126,988 + +1.3 126,989 - -1.0 126,989 - +1.1 126,989 - +1.0 126,989 - +0.7 126,989 - -1.4 126,989 - +1.0 127,050 - Pf6N2E2_82 0.33 +1.2 127,093 + Pf6N2E2_82 0.61 -1.3 127,094 - Pf6N2E2_82 0.62 -2.3 127,094 - Pf6N2E2_82 0.62 -1.7 127,226 + +4.8 127,302 - -0.9 127,349 - +1.0 127,397 + -2.4 127,398 - -1.4 127,398 - +1.4 127,412 + +1.2 127,412 + +0.1 127,412 + -0.2 127,413 - +0.9 127,413 - +0.3 127,413 - +0.4 127,413 - +1.1 127,413 - +1.7 127,413 - +1.6 127,529 + -1.8 127,529 + +2.0 127,529 + -0.2 127,529 + -0.7 127,529 + -0.3 127,530 - -0.6 127,530 - -2.1 127,530 - -0.4 127,530 - +0.3 127,530 - -0.8 127,530 - +0.1 127,530 - +0.3 127,530 - -1.0 127,530 - +0.4 127,568 + +1.2 127,569 - +0.3 127,580 + +1.3 127,580 + -0.7 127,581 - +0.6 127,581 - +0.8 127,649 + Pf6N2E2_83 0.16 -0.2 127,890 + Pf6N2E2_83 0.43 +0.8 127,986 + Pf6N2E2_83 0.54 +0.5 127,986 + Pf6N2E2_83 0.54 -0.0 127,986 + Pf6N2E2_83 0.54 -3.0 127,986 + Pf6N2E2_83 0.54 +2.2 127,986 + Pf6N2E2_83 0.54 +1.1 127,986 + Pf6N2E2_83 0.54 -1.0 127,987 - Pf6N2E2_83 0.54 +1.1 127,987 - Pf6N2E2_83 0.54 -0.8 127,987 - Pf6N2E2_83 0.54 +1.7 127,987 - Pf6N2E2_83 0.54 +1.7 128,140 + Pf6N2E2_83 0.72 +1.8 128,185 + Pf6N2E2_83 0.77 +2.0 128,204 + Pf6N2E2_83 0.79 -0.2 128,204 + Pf6N2E2_83 0.79 +0.6 128,204 + Pf6N2E2_83 0.79 +0.9 128,205 - Pf6N2E2_83 0.80 -2.3 128,205 - Pf6N2E2_83 0.80 +1.4 128,205 - Pf6N2E2_83 0.80 +1.4 128,205 - Pf6N2E2_83 0.80 +0.7 128,286 + Pf6N2E2_83 0.89 +2.2 128,286 + Pf6N2E2_83 0.89 +0.1 128,287 - Pf6N2E2_83 0.89 -2.7 128,287 - Pf6N2E2_83 0.89 +0.1 128,287 - Pf6N2E2_83 0.89 +0.2 128,287 - Pf6N2E2_83 0.89 +1.1 128,319 + -1.9 128,320 - -0.7 128,320 - +0.1 128,324 + +0.6 128,325 - +1.4 128,404 - -1.9 128,404 - +1.6 128,404 - +0.7 128,657 + +1.9 128,658 - -1.7 128,658 - +0.1 128,672 + +0.5 128,673 - +0.5 128,673 - +0.2 128,679 + +1.2 128,680 - -1.8 128,696 + -1.9 128,697 - +1.0 128,697 - -0.5 128,698 + +1.0 128,698 + -0.2 128,699 - -1.2 128,699 - +2.1 128,712 + +0.2 128,764 - -0.0 128,764 - -0.8 128,764 - -2.4 128,765 + +1.3 128,765 + -0.8 128,765 + +1.2 128,765 + +1.1 128,765 + +1.5 128,766 - -2.2 128,766 - -1.0 128,766 - -1.0 128,847 + -1.0 128,847 + -0.8 128,848 - +0.9 128,848 - -0.8 128,945 + +1.1 129,015 - -0.5 129,334 + -0.2 129,358 + +1.3 129,358 + +3.2 129,358 + -2.2 129,359 - +3.8 129,359 - +2.5 129,363 - -0.5 129,368 + -1.5
Or see this region's nucleotide sequence