Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_711 and Pf6N2E2_712 are separated by 4 nucleotides Pf6N2E2_712 and Pf6N2E2_713 overlap by 4 nucleotides Pf6N2E2_713 and Pf6N2E2_714 are separated by 566 nucleotides
Pf6N2E2_711 - Nitric oxide reductase activation protein NorD, at 814,866 to 816,704
_711
Pf6N2E2_712 - hypothetical protein, at 816,709 to 816,909
_712
Pf6N2E2_713 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 816,906 to 817,589
_713
Pf6N2E2_714 - NnrS protein involved in response to NO, at 818,156 to 819,346
_714
Position (kb) 816
817
818 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 816.162 kb on - strand, within Pf6N2E2_711 at 816.267 kb on + strand, within Pf6N2E2_711 at 816.267 kb on + strand, within Pf6N2E2_711 at 816.267 kb on + strand, within Pf6N2E2_711 at 816.268 kb on - strand, within Pf6N2E2_711 at 816.268 kb on - strand, within Pf6N2E2_711 at 816.381 kb on + strand, within Pf6N2E2_711 at 816.381 kb on + strand, within Pf6N2E2_711 at 816.381 kb on + strand, within Pf6N2E2_711 at 816.381 kb on + strand, within Pf6N2E2_711 at 816.382 kb on - strand, within Pf6N2E2_711 at 816.382 kb on - strand, within Pf6N2E2_711 at 816.382 kb on - strand, within Pf6N2E2_711 at 816.382 kb on - strand, within Pf6N2E2_711 at 816.495 kb on + strand, within Pf6N2E2_711 at 816.495 kb on + strand, within Pf6N2E2_711 at 816.495 kb on + strand, within Pf6N2E2_711 at 816.601 kb on - strand at 816.609 kb on + strand at 816.609 kb on + strand at 816.609 kb on + strand at 816.609 kb on + strand at 816.609 kb on + strand at 816.610 kb on - strand at 816.610 kb on - strand at 816.610 kb on - strand at 816.610 kb on - strand at 816.610 kb on - strand at 816.610 kb on - strand at 816.631 kb on - strand at 816.681 kb on + strand at 816.682 kb on - strand at 816.884 kb on + strand, within Pf6N2E2_712 at 816.884 kb on + strand, within Pf6N2E2_712 at 816.885 kb on - strand, within Pf6N2E2_712 at 816.885 kb on - strand, within Pf6N2E2_712 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.243 kb on + strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand at 817.244 kb on - strand, within Pf6N2E2_713 at 817.244 kb on - strand, within Pf6N2E2_713 at 817.247 kb on + strand, within Pf6N2E2_713 at 817.264 kb on + strand, within Pf6N2E2_713 at 817.266 kb on + strand, within Pf6N2E2_713 at 817.266 kb on + strand, within Pf6N2E2_713 at 817.267 kb on - strand, within Pf6N2E2_713 at 817.267 kb on - strand, within Pf6N2E2_713 at 817.303 kb on + strand, within Pf6N2E2_713 at 817.304 kb on - strand, within Pf6N2E2_713 at 817.429 kb on + strand, within Pf6N2E2_713 at 817.605 kb on + strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.606 kb on - strand at 817.798 kb on + strand at 817.799 kb on - strand at 817.799 kb on - strand at 817.861 kb on + strand at 817.878 kb on + strand at 817.989 kb on + strand at 817.990 kb on - strand at 817.990 kb on - strand at 817.993 kb on + strand at 818.117 kb on + strand at 818.117 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.155 kb on + strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.156 kb on - strand at 818.177 kb on - strand at 818.588 kb on + strand, within Pf6N2E2_714
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 816,162 - Pf6N2E2_711 0.70 -1.5 816,267 + Pf6N2E2_711 0.76 +2.5 816,267 + Pf6N2E2_711 0.76 -2.4 816,267 + Pf6N2E2_711 0.76 -3.1 816,268 - Pf6N2E2_711 0.76 -1.8 816,268 - Pf6N2E2_711 0.76 +0.7 816,381 + Pf6N2E2_711 0.82 -0.1 816,381 + Pf6N2E2_711 0.82 -2.4 816,381 + Pf6N2E2_711 0.82 -0.6 816,381 + Pf6N2E2_711 0.82 +2.1 816,382 - Pf6N2E2_711 0.82 +0.3 816,382 - Pf6N2E2_711 0.82 +0.4 816,382 - Pf6N2E2_711 0.82 +0.7 816,382 - Pf6N2E2_711 0.82 +0.5 816,495 + Pf6N2E2_711 0.89 -2.1 816,495 + Pf6N2E2_711 0.89 +1.5 816,495 + Pf6N2E2_711 0.89 -0.3 816,601 - -0.4 816,609 + +0.4 816,609 + -0.3 816,609 + -0.2 816,609 + -1.5 816,609 + +1.4 816,610 - -0.5 816,610 - -1.3 816,610 - +0.0 816,610 - -0.1 816,610 - -1.3 816,610 - -1.1 816,631 - -0.1 816,681 + +2.1 816,682 - +0.1 816,884 + Pf6N2E2_712 0.87 -2.4 816,884 + Pf6N2E2_712 0.87 -0.0 816,885 - Pf6N2E2_712 0.88 +0.5 816,885 - Pf6N2E2_712 0.88 +0.3 817,243 + Pf6N2E2_713 0.49 +0.3 817,243 + Pf6N2E2_713 0.49 +0.9 817,243 + Pf6N2E2_713 0.49 -2.6 817,243 + Pf6N2E2_713 0.49 -0.1 817,243 + Pf6N2E2_713 0.49 -2.9 817,243 + Pf6N2E2_713 0.49 -1.8 817,243 + Pf6N2E2_713 0.49 -0.1 817,244 - Pf6N2E2_713 0.49 +0.8 817,244 - Pf6N2E2_713 0.49 -1.7 817,244 - Pf6N2E2_713 0.49 -1.6 817,244 - +2.7 817,244 - Pf6N2E2_713 0.49 -0.2 817,244 - Pf6N2E2_713 0.49 -0.8 817,247 + Pf6N2E2_713 0.50 +0.5 817,264 + Pf6N2E2_713 0.52 -0.7 817,266 + Pf6N2E2_713 0.53 +0.7 817,266 + Pf6N2E2_713 0.53 -1.1 817,267 - Pf6N2E2_713 0.53 -0.7 817,267 - Pf6N2E2_713 0.53 +0.3 817,303 + Pf6N2E2_713 0.58 +0.7 817,304 - Pf6N2E2_713 0.58 -1.3 817,429 + Pf6N2E2_713 0.76 -2.0 817,605 + +0.8 817,606 - -2.4 817,606 - -0.8 817,606 - -0.3 817,606 - -2.2 817,606 - -0.1 817,798 + +1.4 817,799 - -0.6 817,799 - +0.9 817,861 + +0.9 817,878 + -2.1 817,989 + +0.4 817,990 - +0.6 817,990 - -0.4 817,993 + +0.9 818,117 + -1.8 818,117 + -2.0 818,155 + +0.3 818,155 + +0.9 818,155 + -2.3 818,155 + -1.1 818,155 + -0.9 818,155 + +1.5 818,155 + -0.6 818,155 + -0.3 818,155 + +0.1 818,155 + +1.1 818,155 + +0.5 818,155 + +0.0 818,155 + +0.4 818,155 + -2.0 818,155 + -1.6 818,155 + -0.4 818,155 + -0.2 818,155 + -0.2 818,155 + -2.1 818,155 + +0.2 818,156 - -1.8 818,156 - -2.9 818,156 - -1.7 818,156 - +1.1 818,156 - +0.6 818,156 - +1.6 818,156 - +0.9 818,156 - -0.2 818,156 - -1.7 818,156 - +0.2 818,156 - -1.5 818,156 - +1.5 818,156 - +0.6 818,156 - +0.7 818,177 - +1.1 818,588 + Pf6N2E2_714 0.36 +1.4
Or see this region's nucleotide sequence