Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotides Pf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotides Pf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotides
Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713
_692
Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125
_693
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Position (kb) 800
801
802 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.927 kb on - strand at 799.952 kb on + strand at 799.952 kb on + strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.132 kb on - strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.222 kb on + strand at 800.258 kb on + strand, within Pf6N2E2_694 at 800.273 kb on + strand, within Pf6N2E2_694 at 800.274 kb on - strand, within Pf6N2E2_694 at 800.309 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.480 kb on + strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.814 kb on - strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.864 kb on + strand, within Pf6N2E2_694 at 800.925 kb on - strand, within Pf6N2E2_694 at 801.051 kb on - strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.485 kb on + strand at 801.561 kb on + strand at 801.561 kb on + strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.807 kb on + strand, within Pf6N2E2_695 at 801.957 kb on + strand, within Pf6N2E2_695 at 801.958 kb on - strand, within Pf6N2E2_695 at 802.124 kb on - strand, within Pf6N2E2_695 at 802.224 kb on + strand, within Pf6N2E2_695
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 799,168 + Pf6N2E2_692 0.57 +0.6 799,168 + Pf6N2E2_692 0.57 +0.8 799,927 - -0.8 799,952 + -0.9 799,952 + -0.1 799,953 - -0.5 799,953 - +0.3 799,953 - +0.1 799,990 - -0.8 799,990 - +0.5 799,990 - -1.5 800,055 - Pf6N2E2_693 0.49 -2.3 800,055 - Pf6N2E2_693 0.49 +1.9 800,055 - Pf6N2E2_693 0.49 -2.3 800,132 - +1.4 800,169 + -1.8 800,169 + -2.0 800,169 + +0.8 800,169 + +0.3 800,169 + -1.0 800,170 - -0.8 800,170 - +0.1 800,170 - +1.1 800,170 - +0.4 800,222 + +0.7 800,258 + Pf6N2E2_694 0.11 -0.0 800,273 + Pf6N2E2_694 0.12 -2.7 800,274 - Pf6N2E2_694 0.12 -0.7 800,309 + Pf6N2E2_694 0.15 -3.1 800,354 + Pf6N2E2_694 0.18 -3.0 800,354 + Pf6N2E2_694 0.18 -1.7 800,355 - Pf6N2E2_694 0.18 -2.2 800,355 - Pf6N2E2_694 0.18 +2.5 800,355 - Pf6N2E2_694 0.18 +2.0 800,355 - Pf6N2E2_694 0.18 +1.3 800,480 + Pf6N2E2_694 0.28 +1.6 800,481 - Pf6N2E2_694 0.28 -2.6 800,481 - Pf6N2E2_694 0.28 -2.4 800,481 - Pf6N2E2_694 0.28 -0.3 800,481 - Pf6N2E2_694 0.28 -0.7 800,814 - Pf6N2E2_694 0.54 -1.0 800,843 + Pf6N2E2_694 0.56 -1.0 800,843 + Pf6N2E2_694 0.56 -0.0 800,844 - Pf6N2E2_694 0.56 +0.8 800,844 - Pf6N2E2_694 0.56 +0.3 800,844 - Pf6N2E2_694 0.56 -0.3 800,864 + Pf6N2E2_694 0.57 -0.4 800,925 - Pf6N2E2_694 0.62 -0.5 801,051 - Pf6N2E2_694 0.72 -0.7 801,068 + Pf6N2E2_694 0.73 -1.5 801,068 + Pf6N2E2_694 0.73 -0.5 801,068 + Pf6N2E2_694 0.73 -1.0 801,485 + +0.4 801,561 + +0.7 801,561 + +1.9 801,562 - -1.9 801,562 - +1.0 801,562 - -1.1 801,807 + Pf6N2E2_695 0.20 +2.4 801,957 + Pf6N2E2_695 0.30 +1.3 801,958 - Pf6N2E2_695 0.30 +1.3 802,124 - Pf6N2E2_695 0.41 -0.2 802,224 + Pf6N2E2_695 0.47 +0.5
Or see this region's nucleotide sequence