Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_670 and Pf6N2E2_671 are separated by 214 nucleotides Pf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotides Pf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotides
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017
_672
Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660
_673
Position (kb) 776
777
778 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.417 kb on - strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.588 kb on + strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 776.032 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.042 kb on + strand at 776.043 kb on - strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.058 kb on - strand at 776.058 kb on - strand at 776.061 kb on + strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.117 kb on + strand at 776.117 kb on + strand at 776.141 kb on + strand at 776.328 kb on - strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.739 kb on - strand, within Pf6N2E2_671 at 777.217 kb on + strand at 777.217 kb on + strand at 777.218 kb on - strand at 777.218 kb on - strand at 777.494 kb on - strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.549 kb on - strand, within Pf6N2E2_672 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.081 kb on - strand, within Pf6N2E2_673
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 775,288 + Pf6N2E2_670 0.22 -0.2 775,288 + -1.7 775,289 - Pf6N2E2_670 0.22 +0.1 775,289 - Pf6N2E2_670 0.22 +2.9 775,289 - Pf6N2E2_670 0.22 -0.3 775,416 + Pf6N2E2_670 0.36 -0.9 775,416 + Pf6N2E2_670 0.36 +1.2 775,416 + Pf6N2E2_670 0.36 -1.0 775,417 - Pf6N2E2_670 0.36 -0.3 775,570 + Pf6N2E2_670 0.53 +0.6 775,570 + Pf6N2E2_670 0.53 +0.9 775,571 - Pf6N2E2_670 0.53 -0.5 775,571 - Pf6N2E2_670 0.53 -0.7 775,588 + Pf6N2E2_670 0.55 -2.0 775,589 - Pf6N2E2_670 0.55 -1.9 775,589 - Pf6N2E2_670 0.55 -1.7 775,589 - Pf6N2E2_670 0.55 +1.7 775,663 + Pf6N2E2_670 0.63 -0.5 775,663 + Pf6N2E2_670 0.63 +0.3 776,032 + +0.6 776,034 + +0.2 776,034 + +1.5 776,034 + -1.8 776,034 + -2.7 776,034 + -0.2 776,034 + -1.3 776,034 + -2.3 776,035 - +1.4 776,035 - +1.1 776,035 - +0.1 776,035 - -0.0 776,042 + +0.2 776,043 - +0.4 776,057 + -2.3 776,057 + -1.0 776,057 + -0.0 776,057 + +0.4 776,057 + +1.1 776,058 - +0.7 776,058 - -1.0 776,061 + +0.9 776,062 - +0.7 776,062 - -1.7 776,062 - -1.7 776,117 + -2.3 776,117 + +0.9 776,141 + +0.8 776,328 - Pf6N2E2_671 0.11 -3.1 776,406 + Pf6N2E2_671 0.19 -0.0 776,406 + Pf6N2E2_671 0.19 -2.4 776,454 + Pf6N2E2_671 0.23 -3.3 776,454 + Pf6N2E2_671 0.23 -0.7 776,739 - Pf6N2E2_671 0.51 -0.7 777,217 + -1.9 777,217 + +0.7 777,218 - -0.5 777,218 - +0.2 777,494 - Pf6N2E2_672 0.28 -0.9 777,548 + Pf6N2E2_672 0.35 -0.9 777,548 + Pf6N2E2_672 0.35 +0.5 777,549 - Pf6N2E2_672 0.35 -0.7 778,078 - Pf6N2E2_673 0.10 -0.9 778,078 - Pf6N2E2_673 0.10 +1.1 778,081 - Pf6N2E2_673 0.11 -1.1
Or see this region's nucleotide sequence