Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_666 and Pf6N2E2_667 overlap by 1 nucleotides Pf6N2E2_667 and Pf6N2E2_668 are separated by 55 nucleotides
Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058
_666
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Position (kb) 772
773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 771.378 kb on + strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.526 kb on - strand, within Pf6N2E2_666 at 771.601 kb on - strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.776 kb on - strand, within Pf6N2E2_666 at 771.884 kb on + strand, within Pf6N2E2_666 at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 772.072 kb on + strand at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.389 kb on - strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.590 kb on - strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 771,378 + Pf6N2E2_666 0.33 +0.1 771,379 - Pf6N2E2_666 0.33 +0.9 771,379 - Pf6N2E2_666 0.33 -0.7 771,506 - Pf6N2E2_666 0.46 -1.1 771,506 - Pf6N2E2_666 0.46 +1.2 771,526 - Pf6N2E2_666 0.48 -0.4 771,601 - Pf6N2E2_666 0.55 +0.9 771,633 + Pf6N2E2_666 0.58 -0.1 771,633 + Pf6N2E2_666 0.58 -2.3 771,776 - Pf6N2E2_666 0.72 +0.0 771,884 + Pf6N2E2_666 0.83 +2.2 771,993 + +0.2 771,993 + +1.2 771,993 + -0.2 771,993 + +1.8 771,993 + +1.0 771,994 - +1.7 771,994 - -0.7 771,994 - +1.4 771,994 - +1.5 771,994 - +0.8 772,072 + +0.1 772,782 - Pf6N2E2_667 0.65 -1.6 772,895 + Pf6N2E2_667 0.75 -0.1 772,895 + Pf6N2E2_667 0.75 -2.0 772,896 - Pf6N2E2_667 0.75 -2.2 772,896 - Pf6N2E2_667 0.75 +1.1 772,948 - Pf6N2E2_667 0.80 -2.3 772,989 + Pf6N2E2_667 0.84 -0.7 772,990 - Pf6N2E2_667 0.84 -0.4 773,388 + Pf6N2E2_668 0.15 -2.0 773,388 + Pf6N2E2_668 0.15 -0.4 773,389 - Pf6N2E2_668 0.15 -2.4 773,568 + Pf6N2E2_668 0.31 +1.0 773,568 + Pf6N2E2_668 0.31 +3.1 773,569 - Pf6N2E2_668 0.31 -2.9 773,569 - Pf6N2E2_668 0.31 -3.0 773,572 - Pf6N2E2_668 0.32 -0.9 773,572 - Pf6N2E2_668 0.32 -1.7 773,572 - Pf6N2E2_668 0.32 -0.1 773,572 - Pf6N2E2_668 0.32 +2.2 773,590 - Pf6N2E2_668 0.33 -1.3 773,676 + Pf6N2E2_668 0.41 -1.7 773,676 + Pf6N2E2_668 0.41 -1.5 774,147 + Pf6N2E2_668 0.84 +0.8 774,148 - Pf6N2E2_668 0.84 +0.8 774,148 - Pf6N2E2_668 0.84 +0.4 774,148 - Pf6N2E2_668 0.84 -0.9
Or see this region's nucleotide sequence