Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotides Pf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotides Pf6N2E2_651 and Pf6N2E2_652 are separated by 172 nucleotides
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400
_650
Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760
_651
Pf6N2E2_652 - General secretion pathway protein E, at 741,933 to 743,351
_652
Position (kb) 739
740
741
742 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 738.413 kb on + strand, within Pf6N2E2_649 at 738.834 kb on - strand at 738.834 kb on - strand at 739.008 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.156 kb on + strand, within Pf6N2E2_650 at 739.157 kb on - strand, within Pf6N2E2_650 at 739.597 kb on + strand at 739.946 kb on + strand, within Pf6N2E2_651 at 739.947 kb on - strand, within Pf6N2E2_651 at 740.004 kb on - strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.402 kb on + strand, within Pf6N2E2_651 at 740.522 kb on - strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.279 kb on - strand, within Pf6N2E2_651 at 741.530 kb on + strand at 741.530 kb on + strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.535 kb on + strand at 741.565 kb on + strand at 741.622 kb on + strand at 741.823 kb on + strand at 741.824 kb on - strand at 741.917 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.976 kb on + strand at 742.122 kb on + strand, within Pf6N2E2_652 at 742.123 kb on - strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.702 kb on - strand, within Pf6N2E2_652
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 738,413 + Pf6N2E2_649 0.64 -0.5 738,834 - -2.0 738,834 - -0.9 739,008 + Pf6N2E2_650 0.28 -2.2 739,152 + Pf6N2E2_650 0.55 -2.0 739,152 + Pf6N2E2_650 0.55 +1.0 739,153 - Pf6N2E2_650 0.55 +1.0 739,153 - Pf6N2E2_650 0.55 -1.7 739,153 - Pf6N2E2_650 0.55 +1.3 739,156 + Pf6N2E2_650 0.55 +1.0 739,157 - Pf6N2E2_650 0.56 +1.8 739,597 + +1.0 739,946 + Pf6N2E2_651 0.23 -0.6 739,947 - Pf6N2E2_651 0.23 -1.8 740,004 - Pf6N2E2_651 0.26 +0.9 740,354 + Pf6N2E2_651 0.40 +2.2 740,354 + Pf6N2E2_651 0.40 -0.5 740,354 + Pf6N2E2_651 0.40 -1.5 740,402 + Pf6N2E2_651 0.43 +0.2 740,522 - Pf6N2E2_651 0.48 +1.5 741,155 + Pf6N2E2_651 0.74 -2.9 741,155 + Pf6N2E2_651 0.74 +0.7 741,155 + Pf6N2E2_651 0.74 -2.3 741,156 - Pf6N2E2_651 0.74 -1.4 741,156 - Pf6N2E2_651 0.74 -0.3 741,156 - Pf6N2E2_651 0.74 -1.1 741,156 - Pf6N2E2_651 0.74 -0.9 741,278 + Pf6N2E2_651 0.80 -1.2 741,278 + Pf6N2E2_651 0.80 +1.1 741,278 + Pf6N2E2_651 0.80 -2.1 741,278 + Pf6N2E2_651 0.80 +0.4 741,279 - Pf6N2E2_651 0.80 +0.8 741,530 + -2.2 741,530 + +0.4 741,531 - -1.9 741,531 - -0.6 741,531 - -0.5 741,535 + +0.4 741,565 + -0.4 741,622 + -0.1 741,823 + +0.7 741,824 - -0.5 741,917 + -1.3 741,948 + +0.4 741,948 + -1.0 741,948 + -2.2 741,976 + -2.1 742,122 + Pf6N2E2_652 0.13 +0.7 742,123 - Pf6N2E2_652 0.13 -1.9 742,701 + Pf6N2E2_652 0.54 +0.5 742,701 + Pf6N2E2_652 0.54 +0.3 742,701 + Pf6N2E2_652 0.54 -1.4 742,702 - Pf6N2E2_652 0.54 +0.7
Or see this region's nucleotide sequence