Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_551 and Pf6N2E2_552 overlap by 17 nucleotides Pf6N2E2_552 and Pf6N2E2_553 are separated by 13 nucleotides Pf6N2E2_553 and Pf6N2E2_554 are separated by 197 nucleotides
Pf6N2E2_551 - Protein ImpG/VasA, at 622,878 to 624,668
_551
Pf6N2E2_552 - Uncharacterized protein ImpF, at 624,652 to 625,095
_552
Pf6N2E2_553 - Uncharacterized protein ImpD, at 625,109 to 625,606
_553
Pf6N2E2_554 - Uncharacterized protein ImpC, at 625,804 to 627,285
_554
Position (kb)
625
626 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 624.455 kb on - strand, within Pf6N2E2_551 at 624.631 kb on + strand at 624.631 kb on + strand at 624.631 kb on + strand at 624.631 kb on + strand at 624.632 kb on - strand at 624.635 kb on - strand at 624.674 kb on - strand at 624.757 kb on + strand, within Pf6N2E2_552 at 624.758 kb on - strand, within Pf6N2E2_552 at 624.917 kb on + strand, within Pf6N2E2_552 at 625.110 kb on + strand at 625.296 kb on + strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.389 kb on + strand, within Pf6N2E2_553 at 625.518 kb on + strand, within Pf6N2E2_553 at 625.518 kb on + strand, within Pf6N2E2_553 at 625.519 kb on - strand, within Pf6N2E2_553 at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.654 kb on - strand at 625.654 kb on - strand at 625.767 kb on + strand at 625.767 kb on + strand at 625.767 kb on + strand at 625.768 kb on - strand at 625.775 kb on + strand at 625.776 kb on - strand at 625.783 kb on + strand at 625.783 kb on + strand at 625.783 kb on + strand at 625.783 kb on + strand at 625.783 kb on + strand at 625.784 kb on - strand at 625.784 kb on - strand at 625.791 kb on + strand at 625.791 kb on + strand at 625.814 kb on - strand at 625.902 kb on - strand at 625.902 kb on - strand at 626.003 kb on + strand, within Pf6N2E2_554 at 626.027 kb on + strand, within Pf6N2E2_554 at 626.027 kb on + strand, within Pf6N2E2_554 at 626.028 kb on - strand, within Pf6N2E2_554 at 626.035 kb on + strand, within Pf6N2E2_554 at 626.035 kb on + strand, within Pf6N2E2_554 at 626.035 kb on + strand, within Pf6N2E2_554 at 626.035 kb on + strand, within Pf6N2E2_554 at 626.035 kb on + strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.036 kb on - strand, within Pf6N2E2_554 at 626.090 kb on + strand, within Pf6N2E2_554 at 626.120 kb on + strand, within Pf6N2E2_554 at 626.171 kb on + strand, within Pf6N2E2_554 at 626.171 kb on + strand, within Pf6N2E2_554 at 626.172 kb on - strand, within Pf6N2E2_554 at 626.172 kb on - strand, within Pf6N2E2_554 at 626.172 kb on - strand, within Pf6N2E2_554 at 626.172 kb on - strand, within Pf6N2E2_554 at 626.344 kb on - strand, within Pf6N2E2_554 at 626.344 kb on - strand, within Pf6N2E2_554 at 626.408 kb on + strand, within Pf6N2E2_554 at 626.408 kb on + strand, within Pf6N2E2_554 at 626.408 kb on + strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.409 kb on - strand, within Pf6N2E2_554 at 626.468 kb on + strand, within Pf6N2E2_554 at 626.469 kb on - strand, within Pf6N2E2_554 at 626.469 kb on - strand, within Pf6N2E2_554 at 626.469 kb on - strand, within Pf6N2E2_554 at 626.469 kb on - strand, within Pf6N2E2_554 at 626.469 kb on - strand, within Pf6N2E2_554 at 626.504 kb on + strand, within Pf6N2E2_554 at 626.504 kb on + strand, within Pf6N2E2_554 at 626.505 kb on - strand, within Pf6N2E2_554 at 626.505 kb on - strand, within Pf6N2E2_554 at 626.505 kb on - strand, within Pf6N2E2_554 at 626.505 kb on - strand, within Pf6N2E2_554 at 626.582 kb on + strand, within Pf6N2E2_554
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 624,455 - Pf6N2E2_551 0.88 -1.2 624,631 + -1.1 624,631 + -0.6 624,631 + -2.2 624,631 + -3.4 624,632 - -1.2 624,635 - -0.7 624,674 - -1.2 624,757 + Pf6N2E2_552 0.24 -1.4 624,758 - Pf6N2E2_552 0.24 +1.5 624,917 + Pf6N2E2_552 0.60 -0.4 625,110 + +1.6 625,296 + Pf6N2E2_553 0.38 -2.0 625,297 - Pf6N2E2_553 0.38 +0.9 625,297 - Pf6N2E2_553 0.38 -0.1 625,297 - Pf6N2E2_553 0.38 -0.3 625,389 + Pf6N2E2_553 0.56 +1.0 625,518 + Pf6N2E2_553 0.82 -1.4 625,518 + Pf6N2E2_553 0.82 -2.4 625,519 - Pf6N2E2_553 0.82 -3.2 625,633 + +1.5 625,633 + +0.6 625,633 + -1.2 625,633 + -1.0 625,633 + +0.3 625,634 - +1.4 625,634 - +1.5 625,634 - +0.1 625,634 - -1.9 625,634 - -0.0 625,634 - +0.7 625,654 - +2.7 625,654 - -3.0 625,767 + +1.4 625,767 + +0.2 625,767 + +0.7 625,768 - +1.7 625,775 + +0.0 625,776 - -1.4 625,783 + +1.2 625,783 + +1.1 625,783 + -1.4 625,783 + +0.7 625,783 + +2.0 625,784 - -0.3 625,784 - -0.7 625,791 + +1.3 625,791 + -0.1 625,814 - +2.3 625,902 - -2.6 625,902 - -0.2 626,003 + Pf6N2E2_554 0.13 -2.4 626,027 + Pf6N2E2_554 0.15 +0.7 626,027 + Pf6N2E2_554 0.15 -1.4 626,028 - Pf6N2E2_554 0.15 +0.1 626,035 + Pf6N2E2_554 0.16 +0.1 626,035 + Pf6N2E2_554 0.16 -2.9 626,035 + Pf6N2E2_554 0.16 +1.0 626,035 + Pf6N2E2_554 0.16 +1.1 626,035 + Pf6N2E2_554 0.16 +1.2 626,036 - Pf6N2E2_554 0.16 -0.6 626,036 - Pf6N2E2_554 0.16 +0.5 626,036 - Pf6N2E2_554 0.16 +1.6 626,036 - Pf6N2E2_554 0.16 -2.4 626,036 - Pf6N2E2_554 0.16 +0.1 626,036 - Pf6N2E2_554 0.16 -0.3 626,090 + Pf6N2E2_554 0.19 +0.7 626,120 + Pf6N2E2_554 0.21 -1.3 626,171 + Pf6N2E2_554 0.25 -1.7 626,171 + Pf6N2E2_554 0.25 +1.4 626,172 - Pf6N2E2_554 0.25 -1.7 626,172 - Pf6N2E2_554 0.25 -0.5 626,172 - Pf6N2E2_554 0.25 -0.4 626,172 - Pf6N2E2_554 0.25 -1.0 626,344 - Pf6N2E2_554 0.36 +0.4 626,344 - Pf6N2E2_554 0.36 -0.4 626,408 + Pf6N2E2_554 0.41 +0.0 626,408 + Pf6N2E2_554 0.41 +0.1 626,408 + Pf6N2E2_554 0.41 +0.3 626,409 - Pf6N2E2_554 0.41 +0.3 626,409 - Pf6N2E2_554 0.41 +1.1 626,409 - Pf6N2E2_554 0.41 -0.4 626,409 - Pf6N2E2_554 0.41 +0.6 626,409 - Pf6N2E2_554 0.41 +1.7 626,409 - Pf6N2E2_554 0.41 -1.7 626,468 + Pf6N2E2_554 0.45 -0.3 626,469 - Pf6N2E2_554 0.45 -1.9 626,469 - Pf6N2E2_554 0.45 +1.4 626,469 - Pf6N2E2_554 0.45 +1.0 626,469 - Pf6N2E2_554 0.45 -0.3 626,469 - Pf6N2E2_554 0.45 -1.7 626,504 + Pf6N2E2_554 0.47 +0.5 626,504 + Pf6N2E2_554 0.47 +0.3 626,505 - Pf6N2E2_554 0.47 -2.3 626,505 - Pf6N2E2_554 0.47 -0.3 626,505 - Pf6N2E2_554 0.47 +1.1 626,505 - Pf6N2E2_554 0.47 +0.7 626,582 + Pf6N2E2_554 0.52 -1.7
Or see this region's nucleotide sequence