Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6098 and Pf6N2E2_6099 are separated by 78 nucleotides Pf6N2E2_6099 and Pf6N2E2_54 are separated by 184 nucleotides Pf6N2E2_54 and Pf6N2E2_55 are separated by 28 nucleotides Pf6N2E2_55 and Pf6N2E2_56 are separated by 45 nucleotides Pf6N2E2_56 and Pf6N2E2_57 overlap by 43 nucleotides Pf6N2E2_57 and Pf6N2E2_58 overlap by 6 nucleotides
Pf6N2E2_6098 - tRNA-Ala-GGC, at 94,468 to 94,540
_6098
Pf6N2E2_6099 - tRNA-Glu-TTC, at 94,619 to 94,691
_6099
Pf6N2E2_54 - Transcriptional regulator, TetR family, at 94,876 to 95,415
_54
Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277
_55
Pf6N2E2_56 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 96,323 to 96,772
_56
Pf6N2E2_57 - hypothetical protein, at 96,730 to 96,891
_57
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.546 kb on + strand at 94.546 kb on + strand at 94.663 kb on + strand, within Pf6N2E2_6099 at 94.718 kb on + strand at 94.718 kb on + strand at 94.718 kb on + strand at 95.008 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.144 kb on - strand, within Pf6N2E2_54 at 95.144 kb on - strand, within Pf6N2E2_54 at 95.253 kb on + strand, within Pf6N2E2_54 at 95.418 kb on + strand at 95.419 kb on - strand at 95.419 kb on - strand at 95.468 kb on - strand at 95.626 kb on - strand, within Pf6N2E2_55 at 95.626 kb on - strand, within Pf6N2E2_55 at 95.724 kb on - strand, within Pf6N2E2_55 at 95.724 kb on - strand, within Pf6N2E2_55 at 96.352 kb on + strand at 96.367 kb on - strand at 96.411 kb on + strand, within Pf6N2E2_56 at 96.412 kb on - strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.706 kb on + strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 94,546 + -0.6 94,546 + +0.6 94,663 + Pf6N2E2_6099 0.60 -1.5 94,718 + +0.2 94,718 + +0.6 94,718 + +0.1 95,008 + Pf6N2E2_54 0.24 -0.2 95,143 + Pf6N2E2_54 0.49 -2.0 95,143 + Pf6N2E2_54 0.49 -1.9 95,143 + Pf6N2E2_54 0.49 +0.1 95,144 - Pf6N2E2_54 0.50 -1.4 95,144 - Pf6N2E2_54 0.50 -0.3 95,253 + Pf6N2E2_54 0.70 -0.1 95,418 + +0.3 95,419 - +1.8 95,419 - -0.6 95,468 - +1.1 95,626 - Pf6N2E2_55 0.22 -1.8 95,626 - Pf6N2E2_55 0.22 -0.2 95,724 - Pf6N2E2_55 0.34 -0.1 95,724 - Pf6N2E2_55 0.34 -0.5 96,352 + -0.4 96,367 - -1.7 96,411 + Pf6N2E2_56 0.20 +0.8 96,412 - Pf6N2E2_56 0.20 -1.5 96,413 + Pf6N2E2_56 0.20 +1.0 96,413 + Pf6N2E2_56 0.20 +0.7 96,413 + Pf6N2E2_56 0.20 +1.3 96,413 + Pf6N2E2_56 0.20 -0.4 96,413 + Pf6N2E2_56 0.20 -0.4 96,413 + Pf6N2E2_56 0.20 -1.6 96,413 + Pf6N2E2_56 0.20 -2.1 96,413 + Pf6N2E2_56 0.20 -1.4 96,414 - Pf6N2E2_56 0.20 -0.2 96,414 - +1.1 96,414 - Pf6N2E2_56 0.20 +0.3 96,414 - Pf6N2E2_56 0.20 -0.2 96,414 - Pf6N2E2_56 0.20 -1.9 96,543 - Pf6N2E2_56 0.49 +1.7 96,543 - Pf6N2E2_56 0.49 +0.7 96,706 + Pf6N2E2_56 0.85 -1.7 96,707 - Pf6N2E2_56 0.85 -2.9 96,707 - Pf6N2E2_56 0.85 +1.6 96,707 - Pf6N2E2_56 0.85 +0.1
Or see this region's nucleotide sequence