Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5472 and Pf6N2E2_5473 are separated by 326 nucleotides Pf6N2E2_5473 and Pf6N2E2_5474 are separated by 198 nucleotides
Pf6N2E2_5472 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 6,286,870 to 6,289,182
_5472
Pf6N2E2_5473 - hydrolase, alpha/beta fold family, at 6,289,509 to 6,290,528
_5473
Pf6N2E2_5474 - Glycine cleavage system transcriptional activator, at 6,290,727 to 6,291,608
_5474
Position (kb)
6289
6290
6291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6288.530 kb on - strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.764 kb on - strand, within Pf6N2E2_5472 at 6288.764 kb on - strand, within Pf6N2E2_5472 at 6288.844 kb on + strand, within Pf6N2E2_5472 at 6288.845 kb on - strand, within Pf6N2E2_5472 at 6288.985 kb on + strand at 6289.049 kb on + strand at 6289.050 kb on - strand at 6289.167 kb on + strand at 6289.167 kb on + strand at 6289.275 kb on + strand at 6289.275 kb on + strand at 6289.276 kb on - strand at 6289.276 kb on - strand at 6289.276 kb on - strand at 6289.516 kb on + strand at 6289.517 kb on - strand at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.931 kb on - strand, within Pf6N2E2_5473 at 6290.196 kb on + strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.224 kb on + strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.454 kb on + strand at 6290.455 kb on - strand at 6290.614 kb on + strand at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 6,288,530 - Pf6N2E2_5472 0.72 -1.2 6,288,763 + Pf6N2E2_5472 0.82 -0.4 6,288,763 + Pf6N2E2_5472 0.82 +0.6 6,288,763 + Pf6N2E2_5472 0.82 +0.8 6,288,764 - Pf6N2E2_5472 0.82 -2.4 6,288,764 - Pf6N2E2_5472 0.82 -1.9 6,288,844 + Pf6N2E2_5472 0.85 +0.3 6,288,845 - Pf6N2E2_5472 0.85 +1.3 6,288,985 + +0.1 6,289,049 + -0.0 6,289,050 - +2.0 6,289,167 + -0.3 6,289,167 + +1.1 6,289,275 + -1.8 6,289,275 + +1.5 6,289,276 - +1.4 6,289,276 - +0.3 6,289,276 - -1.5 6,289,516 + +0.2 6,289,517 - -3.2 6,289,860 + Pf6N2E2_5473 0.34 -1.8 6,289,860 + Pf6N2E2_5473 0.34 -3.8 6,289,860 + Pf6N2E2_5473 0.34 -1.2 6,289,861 - Pf6N2E2_5473 0.35 -0.7 6,289,861 - Pf6N2E2_5473 0.35 +2.1 6,289,862 + Pf6N2E2_5473 0.35 +0.1 6,289,862 + Pf6N2E2_5473 0.35 +0.5 6,289,862 + Pf6N2E2_5473 0.35 -0.5 6,289,862 + Pf6N2E2_5473 0.35 -0.3 6,289,862 + Pf6N2E2_5473 0.35 -1.0 6,289,862 + Pf6N2E2_5473 0.35 +0.2 6,289,862 + Pf6N2E2_5473 0.35 +0.4 6,289,862 + Pf6N2E2_5473 0.35 +0.2 6,289,862 + Pf6N2E2_5473 0.35 +0.7 6,289,863 - Pf6N2E2_5473 0.35 -0.6 6,289,863 - Pf6N2E2_5473 0.35 -0.3 6,289,863 - Pf6N2E2_5473 0.35 +0.9 6,289,863 - Pf6N2E2_5473 0.35 -0.0 6,289,863 - Pf6N2E2_5473 0.35 +0.1 6,289,863 - Pf6N2E2_5473 0.35 -0.4 6,289,863 - Pf6N2E2_5473 0.35 +1.8 6,289,930 + Pf6N2E2_5473 0.41 -0.3 6,289,930 + Pf6N2E2_5473 0.41 +0.5 6,289,930 + Pf6N2E2_5473 0.41 -1.3 6,289,931 - Pf6N2E2_5473 0.41 -0.9 6,290,196 + Pf6N2E2_5473 0.67 -3.4 6,290,197 - Pf6N2E2_5473 0.67 -1.3 6,290,197 - Pf6N2E2_5473 0.67 -3.2 6,290,197 - Pf6N2E2_5473 0.67 +1.6 6,290,224 + Pf6N2E2_5473 0.70 -2.5 6,290,225 - Pf6N2E2_5473 0.70 +2.0 6,290,225 - Pf6N2E2_5473 0.70 -0.8 6,290,454 + +2.9 6,290,455 - -1.5 6,290,614 + -0.2 6,291,144 + Pf6N2E2_5474 0.47 -1.9 6,291,144 + Pf6N2E2_5474 0.47 +0.7 6,291,144 + Pf6N2E2_5474 0.47 +0.6 6,291,144 + Pf6N2E2_5474 0.47 +0.5 6,291,144 + Pf6N2E2_5474 0.47 +0.7 6,291,144 + Pf6N2E2_5474 0.47 +1.9 6,291,144 + Pf6N2E2_5474 0.47 +0.2 6,291,144 + Pf6N2E2_5474 0.47 -2.9 6,291,144 + Pf6N2E2_5474 0.47 +0.7 6,291,144 + Pf6N2E2_5474 0.47 +0.9 6,291,144 + Pf6N2E2_5474 0.47 -0.8 6,291,144 + Pf6N2E2_5474 0.47 +1.4 6,291,145 - Pf6N2E2_5474 0.47 +1.6 6,291,145 - Pf6N2E2_5474 0.47 +1.4 6,291,145 - Pf6N2E2_5474 0.47 -0.1 6,291,145 - Pf6N2E2_5474 0.47 +0.9 6,291,145 - Pf6N2E2_5474 0.47 -3.5 6,291,145 - Pf6N2E2_5474 0.47 +0.8 6,291,145 - Pf6N2E2_5474 0.47 -1.1 6,291,145 - Pf6N2E2_5474 0.47 +1.0 6,291,145 - Pf6N2E2_5474 0.47 -1.7
Or see this region's nucleotide sequence