Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5265 and Pf6N2E2_5266 are separated by 137 nucleotides Pf6N2E2_5266 and Pf6N2E2_5267 are separated by 40 nucleotides
Pf6N2E2_5265 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 6,058,908 to 6,060,011
_5265
Pf6N2E2_5266 - Glycosyltransferase, at 6,060,149 to 6,061,348
_5266
Pf6N2E2_5267 - Sulfate transporter, CysZ-type, at 6,061,389 to 6,062,144
_5267
Position (kb)
6060
6061
6062 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6059.183 kb on - strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.673 kb on - strand, within Pf6N2E2_5265 at 6060.009 kb on + strand at 6060.010 kb on - strand at 6060.031 kb on + strand at 6060.032 kb on - strand at 6060.032 kb on - strand at 6060.058 kb on + strand at 6060.058 kb on + strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.367 kb on - strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.982 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.199 kb on + strand, within Pf6N2E2_5266 at 6061.303 kb on + strand at 6061.304 kb on - strand at 6061.609 kb on + strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.720 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6062.014 kb on + strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.114 kb on - strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.221 kb on - strand at 6062.221 kb on - strand at 6062.249 kb on - strand at 6062.269 kb on + strand at 6062.280 kb on + strand at 6062.295 kb on + strand at 6062.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 6,059,183 - Pf6N2E2_5265 0.25 +1.3 6,059,598 + Pf6N2E2_5265 0.62 -0.6 6,059,598 + Pf6N2E2_5265 0.62 -1.5 6,059,673 - Pf6N2E2_5265 0.69 -2.6 6,060,009 + +0.5 6,060,010 - -1.1 6,060,031 + +1.4 6,060,032 - +0.7 6,060,032 - -1.5 6,060,058 + -0.4 6,060,058 + -0.9 6,060,059 - +2.7 6,060,059 - +0.4 6,060,059 - +1.0 6,060,059 - -0.4 6,060,059 - -0.1 6,060,366 + Pf6N2E2_5266 0.18 -1.5 6,060,366 + Pf6N2E2_5266 0.18 -2.2 6,060,366 + Pf6N2E2_5266 0.18 -0.3 6,060,367 - Pf6N2E2_5266 0.18 +0.2 6,060,405 + Pf6N2E2_5266 0.21 -0.7 6,060,405 + Pf6N2E2_5266 0.21 -0.2 6,060,406 - Pf6N2E2_5266 0.21 -1.1 6,060,406 - Pf6N2E2_5266 0.21 +0.1 6,060,501 + Pf6N2E2_5266 0.29 +0.1 6,060,501 + Pf6N2E2_5266 0.29 +0.7 6,060,894 + Pf6N2E2_5266 0.62 +0.1 6,060,894 + Pf6N2E2_5266 0.62 -1.5 6,060,895 - Pf6N2E2_5266 0.62 +0.5 6,060,895 - Pf6N2E2_5266 0.62 -0.3 6,060,895 - Pf6N2E2_5266 0.62 -0.9 6,060,906 - Pf6N2E2_5266 0.63 +2.1 6,060,906 - Pf6N2E2_5266 0.63 -0.0 6,060,942 + Pf6N2E2_5266 0.66 +0.3 6,060,942 + Pf6N2E2_5266 0.66 -2.9 6,060,943 - Pf6N2E2_5266 0.66 +0.4 6,060,943 - Pf6N2E2_5266 0.66 -2.8 6,060,943 - Pf6N2E2_5266 0.66 +0.9 6,060,982 + Pf6N2E2_5266 0.69 +0.1 6,061,041 + Pf6N2E2_5266 0.74 +0.3 6,061,041 + Pf6N2E2_5266 0.74 -0.9 6,061,042 - Pf6N2E2_5266 0.74 +1.1 6,061,042 - Pf6N2E2_5266 0.74 +1.3 6,061,042 - Pf6N2E2_5266 0.74 -0.9 6,061,199 + Pf6N2E2_5266 0.88 +0.6 6,061,303 + +2.1 6,061,304 - +0.6 6,061,609 + Pf6N2E2_5267 0.29 -2.5 6,061,610 - Pf6N2E2_5267 0.29 +1.0 6,061,610 - Pf6N2E2_5267 0.29 -1.3 6,061,610 - Pf6N2E2_5267 0.29 -1.6 6,061,610 - Pf6N2E2_5267 0.29 -2.7 6,061,720 + Pf6N2E2_5267 0.44 +2.1 6,061,936 + Pf6N2E2_5267 0.72 +0.4 6,061,936 + Pf6N2E2_5267 0.72 -4.5 6,061,936 + Pf6N2E2_5267 0.72 -1.7 6,061,936 + Pf6N2E2_5267 0.72 -1.3 6,061,936 + Pf6N2E2_5267 0.72 +0.4 6,061,936 + Pf6N2E2_5267 0.72 +0.3 6,061,936 + Pf6N2E2_5267 0.72 +0.8 6,061,936 + Pf6N2E2_5267 0.72 -1.9 6,061,936 + Pf6N2E2_5267 0.72 +1.4 6,061,937 - Pf6N2E2_5267 0.72 +0.8 6,061,937 - Pf6N2E2_5267 0.72 -0.9 6,061,937 - Pf6N2E2_5267 0.72 +1.0 6,061,937 - Pf6N2E2_5267 0.72 +2.7 6,061,937 - Pf6N2E2_5267 0.72 -3.6 6,061,937 - Pf6N2E2_5267 0.72 +0.4 6,061,937 - Pf6N2E2_5267 0.72 +0.1 6,061,937 - Pf6N2E2_5267 0.72 +0.4 6,061,937 - Pf6N2E2_5267 0.72 +0.6 6,062,014 + Pf6N2E2_5267 0.83 -2.2 6,062,015 - Pf6N2E2_5267 0.83 -0.5 6,062,015 - Pf6N2E2_5267 0.83 -1.1 6,062,061 + Pf6N2E2_5267 0.89 +0.7 6,062,061 + Pf6N2E2_5267 0.89 +0.9 6,062,062 - Pf6N2E2_5267 0.89 +0.8 6,062,062 - Pf6N2E2_5267 0.89 -0.1 6,062,062 - Pf6N2E2_5267 0.89 +0.5 6,062,114 - +0.1 6,062,144 + +0.9 6,062,144 + -2.1 6,062,144 + -0.9 6,062,145 - -0.4 6,062,145 - +1.0 6,062,145 - +0.4 6,062,145 - -3.4 6,062,145 - -1.7 6,062,145 - +2.0 6,062,145 - +0.6 6,062,145 - -3.6 6,062,145 - +1.6 6,062,145 - -0.3 6,062,220 + +1.6 6,062,220 + +3.5 6,062,220 + +0.7 6,062,220 + -0.1 6,062,220 + -1.9 6,062,220 + -1.5 6,062,220 + -1.4 6,062,221 - -0.5 6,062,221 - +1.6 6,062,249 - +0.6 6,062,269 + +1.0 6,062,280 + +1.1 6,062,295 + +1.5 6,062,295 + +0.8
Or see this region's nucleotide sequence