Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_51

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_50 and Pf6N2E2_51 are separated by 15 nucleotidesPf6N2E2_51 and Pf6N2E2_52 are separated by 121 nucleotides Pf6N2E2_50 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 88,788 to 89,984 _50 Pf6N2E2_51 - hypothetical protein, at 90,000 to 90,194 _51 Pf6N2E2_52 - Excinuclease ABC subunit B, at 90,316 to 92,331 _52 Position (kb) 89 90 91Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 89.131 kb on + strand, within Pf6N2E2_50at 89.131 kb on + strand, within Pf6N2E2_50at 89.132 kb on - strand, within Pf6N2E2_50at 89.530 kb on + strand, within Pf6N2E2_50at 89.531 kb on - strand, within Pf6N2E2_50at 89.758 kb on + strand, within Pf6N2E2_50at 89.759 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.852 kb on + strand, within Pf6N2E2_50at 89.852 kb on + strand, within Pf6N2E2_50at 89.853 kb on - strand, within Pf6N2E2_50at 89.853 kb on - strand, within Pf6N2E2_50at 89.872 kb on + strandat 89.872 kb on + strandat 89.873 kb on - strandat 89.903 kb on + strandat 89.985 kb on + strandat 89.986 kb on - strandat 89.998 kb on + strandat 89.999 kb on - strandat 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.069 kb on - strand, within Pf6N2E2_51at 90.069 kb on - strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.071 kb on - strand, within Pf6N2E2_51at 90.178 kb on + strandat 90.178 kb on + strandat 90.310 kb on + strandat 90.310 kb on + strandat 90.310 kb on + strandat 90.311 kb on - strandat 90.332 kb on + strandat 90.332 kb on + strandat 90.333 kb on - strandat 90.532 kb on + strand, within Pf6N2E2_52at 90.532 kb on + strand, within Pf6N2E2_52at 90.574 kb on + strand, within Pf6N2E2_52at 90.574 kb on + strand, within Pf6N2E2_52at 90.575 kb on - strand, within Pf6N2E2_52at 90.586 kb on + strand, within Pf6N2E2_52at 90.587 kb on - strand, within Pf6N2E2_52at 90.595 kb on + strand, within Pf6N2E2_52at 90.595 kb on + strand, within Pf6N2E2_52at 90.598 kb on + strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.613 kb on + strand, within Pf6N2E2_52at 90.614 kb on - strand, within Pf6N2E2_52at 90.614 kb on - strand, within Pf6N2E2_52at 90.748 kb on + strand, within Pf6N2E2_52at 90.748 kb on + strand, within Pf6N2E2_52at 90.749 kb on - strand, within Pf6N2E2_52at 90.772 kb on + strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.854 kb on - strand, within Pf6N2E2_52at 90.854 kb on - strand, within Pf6N2E2_52at 91.043 kb on - strand, within Pf6N2E2_52at 91.057 kb on + strand, within Pf6N2E2_52at 91.058 kb on - strand, within Pf6N2E2_52at 91.058 kb on - strand, within Pf6N2E2_52at 91.129 kb on + strand, within Pf6N2E2_52at 91.130 kb on - strand, within Pf6N2E2_52

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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89,131 + Pf6N2E2_50 0.29 -1.0
89,131 + Pf6N2E2_50 0.29 -2.2
89,132 - Pf6N2E2_50 0.29 -2.2
89,530 + Pf6N2E2_50 0.62 -1.4
89,531 - Pf6N2E2_50 0.62 -1.5
89,758 + Pf6N2E2_50 0.81 -3.3
89,759 - Pf6N2E2_50 0.81 -1.2
89,786 - Pf6N2E2_50 0.83 -0.9
89,786 - Pf6N2E2_50 0.83 -1.7
89,786 - Pf6N2E2_50 0.83 -2.0
89,786 - Pf6N2E2_50 0.83 +0.3
89,852 + Pf6N2E2_50 0.89 -1.0
89,852 + Pf6N2E2_50 0.89 -2.5
89,853 - Pf6N2E2_50 0.89 -1.7
89,853 - Pf6N2E2_50 0.89 -0.9
89,872 + -1.5
89,872 + -1.8
89,873 - -2.3
89,903 + -1.4
89,985 + -0.7
89,986 - -1.5
89,998 + -1.3
89,999 - -0.6
90,065 + Pf6N2E2_51 0.33 -2.0
90,065 + Pf6N2E2_51 0.33 -3.0
90,065 + Pf6N2E2_51 0.33 -1.5
90,065 + Pf6N2E2_51 0.33 -0.0
90,065 + Pf6N2E2_51 0.33 -1.4
90,065 + Pf6N2E2_51 0.33 -2.0
90,066 - Pf6N2E2_51 0.34 -0.5
90,066 - Pf6N2E2_51 0.34 -1.7
90,066 - Pf6N2E2_51 0.34 -0.3
90,066 - Pf6N2E2_51 0.34 -1.9
90,066 - Pf6N2E2_51 0.34 -2.2
90,066 - Pf6N2E2_51 0.34 +0.1
90,066 - Pf6N2E2_51 0.34 -0.7
90,066 - Pf6N2E2_51 0.34 -2.4
90,066 - Pf6N2E2_51 0.34 -1.7
90,066 - Pf6N2E2_51 0.34 -1.2
90,068 + Pf6N2E2_51 0.35 -1.9
90,068 + Pf6N2E2_51 0.35 +0.2
90,068 + Pf6N2E2_51 0.35 -1.5
90,068 + Pf6N2E2_51 0.35 -2.2
90,069 - Pf6N2E2_51 0.35 +0.3
90,069 - Pf6N2E2_51 0.35 +0.8
90,070 + Pf6N2E2_51 0.36 -2.3
90,070 + Pf6N2E2_51 0.36 -2.2
90,070 + Pf6N2E2_51 0.36 -0.8
90,070 + Pf6N2E2_51 0.36 -0.2
90,071 - Pf6N2E2_51 0.36 -1.2
90,178 + -1.9
90,178 + +2.6
90,310 + -0.3
90,310 + -3.3
90,310 + -1.9
90,311 - -0.8
90,332 + -1.2
90,332 + -0.3
90,333 - +0.2
90,532 + Pf6N2E2_52 0.11 +0.4
90,532 + Pf6N2E2_52 0.11 -2.0
90,574 + Pf6N2E2_52 0.13 -0.7
90,574 + Pf6N2E2_52 0.13 +2.1
90,575 - Pf6N2E2_52 0.13 +2.1
90,586 + Pf6N2E2_52 0.13 +1.8
90,587 - Pf6N2E2_52 0.13 -0.7
90,595 + Pf6N2E2_52 0.14 +1.0
90,595 + Pf6N2E2_52 0.14 -1.1
90,598 + Pf6N2E2_52 0.14 -0.7
90,599 - Pf6N2E2_52 0.14 -0.5
90,599 - Pf6N2E2_52 0.14 +1.1
90,599 - Pf6N2E2_52 0.14 -1.5
90,613 + Pf6N2E2_52 0.15 -1.2
90,614 - Pf6N2E2_52 0.15 -0.3
90,614 - Pf6N2E2_52 0.15 +0.8
90,748 + Pf6N2E2_52 0.21 +0.1
90,748 + Pf6N2E2_52 0.21 -1.9
90,749 - Pf6N2E2_52 0.21 +0.7
90,772 + Pf6N2E2_52 0.23 -0.1
90,773 - Pf6N2E2_52 0.23 +0.3
90,773 - Pf6N2E2_52 0.23 +0.9
90,773 - Pf6N2E2_52 0.23 -1.5
90,853 + Pf6N2E2_52 0.27 +1.8
90,853 + Pf6N2E2_52 0.27 +0.2
90,853 + Pf6N2E2_52 0.27 +1.8
90,854 - Pf6N2E2_52 0.27 -2.4
90,854 - Pf6N2E2_52 0.27 -1.7
91,043 - Pf6N2E2_52 0.36 +0.7
91,057 + Pf6N2E2_52 0.37 -1.2
91,058 - Pf6N2E2_52 0.37 -1.1
91,058 - Pf6N2E2_52 0.37 +1.0
91,129 + Pf6N2E2_52 0.40 -0.6
91,130 - Pf6N2E2_52 0.40 -1.5

Or see this region's nucleotide sequence