Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5006 and Pf6N2E2_5007 are separated by 112 nucleotides Pf6N2E2_5007 and Pf6N2E2_5008 are separated by 72 nucleotides Pf6N2E2_5008 and Pf6N2E2_5009 are separated by 276 nucleotides
Pf6N2E2_5006 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 5,771,651 to 5,772,595
_5006
Pf6N2E2_5007 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 5,772,708 to 5,774,030
_5007
Pf6N2E2_5008 - FIG00957593: hypothetical protein, at 5,774,103 to 5,774,267
_5008
Pf6N2E2_5009 - DNA polymerase III delta subunit (EC 2.7.7.7), at 5,774,544 to 5,775,581
_5009
Position (kb)
5772
5773
5774
5775 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.853 kb on + strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.996 kb on + strand, within Pf6N2E2_5006 at 5771.997 kb on - strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.177 kb on - strand, within Pf6N2E2_5006 at 5772.323 kb on + strand, within Pf6N2E2_5006 at 5772.378 kb on + strand, within Pf6N2E2_5006 at 5772.594 kb on - strand at 5772.601 kb on + strand at 5772.602 kb on - strand at 5772.602 kb on - strand at 5772.700 kb on - strand at 5772.922 kb on + strand, within Pf6N2E2_5007 at 5772.923 kb on - strand, within Pf6N2E2_5007 at 5772.958 kb on - strand, within Pf6N2E2_5007 at 5772.976 kb on + strand, within Pf6N2E2_5007 at 5772.977 kb on - strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.995 kb on - strand, within Pf6N2E2_5007 at 5773.111 kb on + strand, within Pf6N2E2_5007 at 5773.112 kb on - strand, within Pf6N2E2_5007 at 5773.177 kb on + strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.675 kb on + strand, within Pf6N2E2_5007 at 5773.676 kb on - strand, within Pf6N2E2_5007 at 5773.676 kb on - strand, within Pf6N2E2_5007 at 5773.985 kb on + strand at 5773.985 kb on + strand at 5774.067 kb on - strand at 5774.085 kb on - strand at 5774.381 kb on - strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.392 kb on - strand at 5774.392 kb on - strand at 5774.411 kb on - strand at 5774.443 kb on + strand at 5774.474 kb on - strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 5,771,831 + Pf6N2E2_5006 0.19 -1.9 5,771,831 + Pf6N2E2_5006 0.19 -2.7 5,771,831 + Pf6N2E2_5006 0.19 -3.4 5,771,832 - Pf6N2E2_5006 0.19 -3.1 5,771,832 - Pf6N2E2_5006 0.19 -2.5 5,771,832 - Pf6N2E2_5006 0.19 -1.8 5,771,853 + Pf6N2E2_5006 0.21 -2.9 5,771,854 - Pf6N2E2_5006 0.21 +0.3 5,771,854 - Pf6N2E2_5006 0.21 -2.8 5,771,991 + Pf6N2E2_5006 0.36 -2.8 5,771,991 + Pf6N2E2_5006 0.36 -2.0 5,771,996 + Pf6N2E2_5006 0.37 -2.1 5,771,997 - Pf6N2E2_5006 0.37 -3.1 5,772,159 + Pf6N2E2_5006 0.54 -2.7 5,772,159 + Pf6N2E2_5006 0.54 -3.8 5,772,160 - Pf6N2E2_5006 0.54 -2.4 5,772,160 - Pf6N2E2_5006 0.54 -1.3 5,772,160 - Pf6N2E2_5006 0.54 -2.5 5,772,176 + Pf6N2E2_5006 0.56 -2.8 5,772,176 + Pf6N2E2_5006 0.56 -2.8 5,772,177 - Pf6N2E2_5006 0.56 -1.7 5,772,323 + Pf6N2E2_5006 0.71 -2.3 5,772,378 + Pf6N2E2_5006 0.77 +0.7 5,772,594 - -1.4 5,772,601 + -2.3 5,772,602 - -2.9 5,772,602 - -1.2 5,772,700 - +0.5 5,772,922 + Pf6N2E2_5007 0.16 -2.2 5,772,923 - Pf6N2E2_5007 0.16 -0.1 5,772,958 - Pf6N2E2_5007 0.19 +1.7 5,772,976 + Pf6N2E2_5007 0.20 -2.1 5,772,977 - Pf6N2E2_5007 0.20 -0.7 5,772,994 + Pf6N2E2_5007 0.22 -2.7 5,772,994 + Pf6N2E2_5007 0.22 -2.6 5,772,994 + Pf6N2E2_5007 0.22 -1.4 5,772,994 + Pf6N2E2_5007 0.22 -2.7 5,772,994 + Pf6N2E2_5007 0.22 +0.8 5,772,994 + Pf6N2E2_5007 0.22 -2.4 5,772,995 - Pf6N2E2_5007 0.22 -2.9 5,773,111 + Pf6N2E2_5007 0.30 -1.7 5,773,112 - Pf6N2E2_5007 0.31 -2.1 5,773,177 + Pf6N2E2_5007 0.35 -0.9 5,773,250 - Pf6N2E2_5007 0.41 -2.9 5,773,250 - Pf6N2E2_5007 0.41 -2.8 5,773,336 + Pf6N2E2_5007 0.47 -1.3 5,773,336 + Pf6N2E2_5007 0.47 -3.2 5,773,336 + Pf6N2E2_5007 0.47 +0.7 5,773,336 + Pf6N2E2_5007 0.47 -0.3 5,773,336 + Pf6N2E2_5007 0.47 -3.5 5,773,337 - Pf6N2E2_5007 0.48 -1.6 5,773,337 - Pf6N2E2_5007 0.48 -0.5 5,773,337 - Pf6N2E2_5007 0.48 -1.1 5,773,514 + Pf6N2E2_5007 0.61 -2.2 5,773,514 + Pf6N2E2_5007 0.61 -2.6 5,773,596 - Pf6N2E2_5007 0.67 -3.0 5,773,596 - Pf6N2E2_5007 0.67 -2.2 5,773,596 - Pf6N2E2_5007 0.67 -1.4 5,773,596 - Pf6N2E2_5007 0.67 -0.8 5,773,675 + Pf6N2E2_5007 0.73 -0.1 5,773,676 - Pf6N2E2_5007 0.73 -1.2 5,773,676 - Pf6N2E2_5007 0.73 -2.9 5,773,985 + -2.1 5,773,985 + -1.1 5,774,067 - -2.5 5,774,085 - -0.1 5,774,381 - +0.3 5,774,391 + +0.7 5,774,391 + -1.3 5,774,391 + -0.4 5,774,391 + +0.2 5,774,392 - +1.7 5,774,392 - +0.9 5,774,411 - +1.1 5,774,443 + +1.3 5,774,474 - +0.7 5,774,519 + -0.5 5,774,519 + -2.8 5,774,519 + +1.3 5,774,519 + -0.7 5,774,519 + -0.0
Or see this region's nucleotide sequence