Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4979 and Pf6N2E2_4980 are separated by 68 nucleotides Pf6N2E2_4980 and Pf6N2E2_4981 overlap by 27 nucleotides Pf6N2E2_4981 and Pf6N2E2_4982 are separated by 362 nucleotides
Pf6N2E2_4979 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,744,978 to 5,746,498
_4979
Pf6N2E2_4980 - hypothetical protein, at 5,746,567 to 5,747,094
_4980
Pf6N2E2_4981 - Nitrogen regulation protein NR(I), at 5,747,068 to 5,748,975
_4981
Pf6N2E2_4982 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 5,749,338 to 5,750,687
_4982
Position (kb)
5747
5748
5749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.438 kb on + strand at 5746.443 kb on + strand at 5746.443 kb on + strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.465 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.526 kb on - strand at 5746.526 kb on - strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.568 kb on + strand at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.785 kb on - strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.787 kb on - strand, within Pf6N2E2_4980 at 5746.956 kb on + strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5747.059 kb on + strand at 5747.059 kb on + strand at 5747.062 kb on + strand at 5747.062 kb on + strand at 5747.063 kb on - strand at 5747.063 kb on - strand at 5747.063 kb on - strand at 5747.699 kb on - strand, within Pf6N2E2_4981 at 5747.847 kb on + strand, within Pf6N2E2_4981 at 5747.847 kb on + strand, within Pf6N2E2_4981 at 5747.848 kb on - strand, within Pf6N2E2_4981 at 5747.848 kb on - strand, within Pf6N2E2_4981 at 5747.927 kb on + strand, within Pf6N2E2_4981 at 5747.927 kb on + strand, within Pf6N2E2_4981 at 5747.927 kb on + strand, within Pf6N2E2_4981 at 5747.927 kb on + strand, within Pf6N2E2_4981 at 5747.928 kb on - strand, within Pf6N2E2_4981 at 5747.928 kb on - strand, within Pf6N2E2_4981 at 5747.928 kb on - strand, within Pf6N2E2_4981 at 5747.928 kb on - strand, within Pf6N2E2_4981 at 5747.928 kb on - strand, within Pf6N2E2_4981 at 5748.231 kb on + strand, within Pf6N2E2_4981 at 5748.231 kb on + strand, within Pf6N2E2_4981 at 5748.231 kb on + strand, within Pf6N2E2_4981 at 5748.232 kb on - strand, within Pf6N2E2_4981 at 5748.232 kb on - strand, within Pf6N2E2_4981 at 5748.232 kb on - strand, within Pf6N2E2_4981 at 5748.538 kb on + strand, within Pf6N2E2_4981 at 5748.538 kb on + strand, within Pf6N2E2_4981 at 5748.538 kb on + strand, within Pf6N2E2_4981 at 5748.538 kb on + strand, within Pf6N2E2_4981 at 5748.538 kb on + strand, within Pf6N2E2_4981 at 5748.539 kb on - strand, within Pf6N2E2_4981 at 5748.539 kb on - strand, within Pf6N2E2_4981 at 5748.539 kb on - strand, within Pf6N2E2_4981 at 5748.539 kb on - strand, within Pf6N2E2_4981 at 5748.539 kb on - strand, within Pf6N2E2_4981 at 5748.717 kb on + strand, within Pf6N2E2_4981 at 5748.717 kb on + strand, within Pf6N2E2_4981 at 5748.717 kb on + strand, within Pf6N2E2_4981 at 5748.717 kb on + strand, within Pf6N2E2_4981 at 5748.718 kb on - strand, within Pf6N2E2_4981 at 5748.930 kb on + strand at 5748.978 kb on + strand at 5748.978 kb on + strand at 5748.978 kb on + strand at 5748.979 kb on - strand at 5748.979 kb on - strand at 5748.979 kb on - strand at 5749.147 kb on + strand at 5749.147 kb on + strand at 5749.147 kb on + strand at 5749.147 kb on + strand at 5749.147 kb on + strand at 5749.148 kb on - strand at 5749.148 kb on - strand at 5749.148 kb on - strand at 5749.148 kb on - strand at 5749.249 kb on + strand at 5749.249 kb on + strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.250 kb on - strand at 5749.268 kb on + strand at 5749.268 kb on + strand at 5749.268 kb on + strand at 5749.268 kb on + strand at 5749.268 kb on + strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.269 kb on - strand at 5749.303 kb on + strand at 5749.325 kb on + strand at 5749.349 kb on + strand at 5749.349 kb on + strand at 5749.349 kb on + strand at 5749.575 kb on + strand, within Pf6N2E2_4982 at 5749.575 kb on + strand, within Pf6N2E2_4982 at 5749.575 kb on + strand, within Pf6N2E2_4982 at 5749.599 kb on + strand, within Pf6N2E2_4982 at 5749.599 kb on + strand, within Pf6N2E2_4982
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 5,746,384 + +1.1 5,746,384 + -2.0 5,746,384 + -2.4 5,746,384 + +0.2 5,746,384 + -1.0 5,746,385 - -0.3 5,746,385 - -2.7 5,746,385 - +0.9 5,746,385 - +0.4 5,746,385 - -0.1 5,746,385 - -1.0 5,746,385 - +0.9 5,746,385 - +3.2 5,746,385 - -0.5 5,746,385 - +0.1 5,746,385 - +1.0 5,746,395 + +0.1 5,746,395 + +1.2 5,746,395 + +1.6 5,746,395 + -1.4 5,746,395 + -1.6 5,746,396 - +0.8 5,746,396 - -2.0 5,746,396 - +3.0 5,746,396 - -2.0 5,746,434 + -0.3 5,746,434 + +0.9 5,746,434 + -1.4 5,746,434 + -1.6 5,746,434 + +1.5 5,746,435 - +0.6 5,746,435 - +0.6 5,746,435 - -2.4 5,746,435 - +1.2 5,746,435 - +1.0 5,746,435 - +1.2 5,746,438 + -1.4 5,746,443 + -1.9 5,746,443 + +0.7 5,746,444 - +3.1 5,746,444 - -2.1 5,746,444 - +0.3 5,746,444 - -2.7 5,746,465 + +0.8 5,746,525 + +1.1 5,746,525 + -0.9 5,746,525 + -1.6 5,746,526 - +0.8 5,746,526 - -0.8 5,746,527 + +0.8 5,746,527 + -2.9 5,746,527 + -0.7 5,746,528 - +1.1 5,746,528 - -2.0 5,746,528 - +0.5 5,746,562 + +0.1 5,746,562 + +0.3 5,746,562 + -0.6 5,746,563 - -0.8 5,746,563 - +2.4 5,746,563 - +1.6 5,746,568 + +1.4 5,746,784 + Pf6N2E2_4980 0.41 -2.0 5,746,784 + Pf6N2E2_4980 0.41 -1.3 5,746,784 + Pf6N2E2_4980 0.41 +1.3 5,746,785 - Pf6N2E2_4980 0.41 -0.1 5,746,786 + Pf6N2E2_4980 0.41 +1.2 5,746,786 + Pf6N2E2_4980 0.41 -1.7 5,746,786 + Pf6N2E2_4980 0.41 -3.0 5,746,786 + Pf6N2E2_4980 0.41 -0.3 5,746,786 + Pf6N2E2_4980 0.41 -1.0 5,746,786 + Pf6N2E2_4980 0.41 -0.3 5,746,787 - Pf6N2E2_4980 0.42 -1.4 5,746,956 + Pf6N2E2_4980 0.74 +1.2 5,746,957 - Pf6N2E2_4980 0.74 -2.9 5,746,957 - Pf6N2E2_4980 0.74 +0.7 5,746,957 - Pf6N2E2_4980 0.74 +0.3 5,747,059 + +0.1 5,747,059 + -0.0 5,747,062 + -2.0 5,747,062 + -0.6 5,747,063 - -3.2 5,747,063 - +2.6 5,747,063 - -0.4 5,747,699 - Pf6N2E2_4981 0.33 -0.4 5,747,847 + Pf6N2E2_4981 0.41 +1.9 5,747,847 + Pf6N2E2_4981 0.41 -0.7 5,747,848 - Pf6N2E2_4981 0.41 +1.2 5,747,848 - Pf6N2E2_4981 0.41 -1.1 5,747,927 + Pf6N2E2_4981 0.45 +1.7 5,747,927 + Pf6N2E2_4981 0.45 +1.5 5,747,927 + Pf6N2E2_4981 0.45 -1.3 5,747,927 + Pf6N2E2_4981 0.45 -0.2 5,747,928 - Pf6N2E2_4981 0.45 -0.1 5,747,928 - Pf6N2E2_4981 0.45 -0.6 5,747,928 - Pf6N2E2_4981 0.45 +1.7 5,747,928 - Pf6N2E2_4981 0.45 +0.5 5,747,928 - Pf6N2E2_4981 0.45 +0.2 5,748,231 + Pf6N2E2_4981 0.61 -0.2 5,748,231 + Pf6N2E2_4981 0.61 -0.9 5,748,231 + Pf6N2E2_4981 0.61 +0.4 5,748,232 - Pf6N2E2_4981 0.61 -0.5 5,748,232 - Pf6N2E2_4981 0.61 -1.0 5,748,232 - Pf6N2E2_4981 0.61 +0.6 5,748,538 + Pf6N2E2_4981 0.77 -1.9 5,748,538 + Pf6N2E2_4981 0.77 -1.6 5,748,538 + Pf6N2E2_4981 0.77 -1.7 5,748,538 + Pf6N2E2_4981 0.77 -1.1 5,748,538 + Pf6N2E2_4981 0.77 +1.4 5,748,539 - Pf6N2E2_4981 0.77 -0.0 5,748,539 - Pf6N2E2_4981 0.77 -1.3 5,748,539 - Pf6N2E2_4981 0.77 -0.2 5,748,539 - Pf6N2E2_4981 0.77 -0.9 5,748,539 - Pf6N2E2_4981 0.77 -0.4 5,748,717 + Pf6N2E2_4981 0.86 +1.0 5,748,717 + Pf6N2E2_4981 0.86 -0.9 5,748,717 + Pf6N2E2_4981 0.86 -0.8 5,748,717 + Pf6N2E2_4981 0.86 -0.3 5,748,718 - Pf6N2E2_4981 0.86 -0.7 5,748,930 + -2.2 5,748,978 + -1.1 5,748,978 + -2.4 5,748,978 + +0.8 5,748,979 - -1.3 5,748,979 - +1.1 5,748,979 - +0.3 5,749,147 + +0.6 5,749,147 + +0.1 5,749,147 + +1.8 5,749,147 + -1.4 5,749,147 + +0.2 5,749,148 - -0.1 5,749,148 - -0.8 5,749,148 - +0.9 5,749,148 - +1.3 5,749,249 + -0.3 5,749,249 + -2.2 5,749,250 - -2.6 5,749,250 - +0.2 5,749,250 - -0.2 5,749,250 - -0.1 5,749,250 - -0.4 5,749,250 - -0.0 5,749,250 - +0.6 5,749,250 - +0.3 5,749,268 + -0.1 5,749,268 + -1.3 5,749,268 + -0.7 5,749,268 + -0.2 5,749,268 + +1.1 5,749,269 - +0.7 5,749,269 - -0.0 5,749,269 - +0.5 5,749,269 - +0.0 5,749,269 - +0.1 5,749,269 - +1.3 5,749,269 - +0.5 5,749,303 + +0.3 5,749,325 + +0.6 5,749,349 + -2.3 5,749,349 + -2.6 5,749,349 + -1.1 5,749,575 + Pf6N2E2_4982 0.18 +1.9 5,749,575 + Pf6N2E2_4982 0.18 -1.7 5,749,575 + Pf6N2E2_4982 0.18 +1.0 5,749,599 + Pf6N2E2_4982 0.19 +0.6 5,749,599 + Pf6N2E2_4982 0.19 -1.1
Or see this region's nucleotide sequence