Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_495

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_493 and Pf6N2E2_494 are separated by 149 nucleotidesPf6N2E2_494 and Pf6N2E2_495 are separated by 29 nucleotidesPf6N2E2_495 and Pf6N2E2_496 are separated by 183 nucleotides Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831 _493 Pf6N2E2_494 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 560,981 to 561,418 _494 Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563 _495 Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) (from data), at 562,747 to 564,021 _496 Position (kb) 561 562 563Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 560.452 kb on + strand, within Pf6N2E2_493at 560.452 kb on + strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.701 kb on - strand, within Pf6N2E2_493at 560.729 kb on - strandat 560.729 kb on - strandat 560.729 kb on - strandat 560.894 kb on - strandat 560.896 kb on + strandat 560.908 kb on + strandat 560.909 kb on - strandat 560.909 kb on - strandat 560.927 kb on - strandat 560.946 kb on + strandat 560.946 kb on + strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.947 kb on - strandat 560.949 kb on + strandat 560.949 kb on + strandat 560.949 kb on + strandat 560.950 kb on - strandat 560.950 kb on - strandat 560.971 kb on + strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.972 kb on - strandat 560.973 kb on + strandat 560.973 kb on + strandat 561.065 kb on + strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.134 kb on - strand, within Pf6N2E2_494at 561.180 kb on - strand, within Pf6N2E2_494at 561.453 kb on - strandat 561.573 kb on + strand, within Pf6N2E2_495at 561.637 kb on + strand, within Pf6N2E2_495at 561.834 kb on + strand, within Pf6N2E2_495at 562.001 kb on + strand, within Pf6N2E2_495at 562.085 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.607 kb on + strandat 562.625 kb on + strandat 562.628 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.641 kb on - strandat 562.764 kb on - strandat 562.867 kb on - strandat 562.892 kb on - strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.899 kb on - strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.903 kb on - strandat 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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560,452 + Pf6N2E2_493 0.64 -2.2
560,452 + Pf6N2E2_493 0.64 -0.1
560,701 - Pf6N2E2_493 0.87 -2.1
560,701 - Pf6N2E2_493 0.87 +1.9
560,701 - Pf6N2E2_493 0.87 +0.0
560,701 - Pf6N2E2_493 0.87 +0.6
560,729 - -0.1
560,729 - -1.1
560,729 - -1.6
560,894 - -1.3
560,896 + +1.2
560,908 + +0.2
560,909 - +1.3
560,909 - +0.3
560,927 - -1.8
560,946 + +1.9
560,946 + +1.8
560,947 - -2.9
560,947 - +1.6
560,947 - -0.4
560,947 - +1.1
560,947 - -1.0
560,949 + -0.6
560,949 + +1.4
560,949 + -2.0
560,950 - +0.1
560,950 - +0.5
560,971 + -0.6
560,972 - +1.2
560,972 - +1.7
560,972 - -1.1
560,972 - +1.1
560,973 + +0.7
560,973 + +1.4
561,065 + Pf6N2E2_494 0.19 +1.3
561,134 - Pf6N2E2_494 0.35 +2.1
561,134 - Pf6N2E2_494 0.35 +0.7
561,134 - Pf6N2E2_494 0.35 -2.5
561,180 - Pf6N2E2_494 0.45 -1.3
561,453 - +3.3
561,573 + Pf6N2E2_495 0.11 -1.8
561,637 + Pf6N2E2_495 0.17 -3.4
561,834 + Pf6N2E2_495 0.35 +1.3
562,001 + Pf6N2E2_495 0.50 +2.0
562,085 + Pf6N2E2_495 0.57 -3.2
562,184 + Pf6N2E2_495 0.66 +2.2
562,184 + Pf6N2E2_495 0.66 +1.4
562,607 + -1.4
562,625 + -1.3
562,628 + -1.8
562,640 + -1.0
562,640 + +2.1
562,640 + +1.2
562,640 + +0.7
562,641 - -0.9
562,764 - -3.2
562,867 - -1.0
562,892 - Pf6N2E2_496 0.11 -2.0
562,898 + Pf6N2E2_496 0.12 +0.7
562,898 + Pf6N2E2_496 0.12 +0.5
562,898 + Pf6N2E2_496 0.12 -3.6
562,898 + Pf6N2E2_496 0.12 -1.1
562,898 + Pf6N2E2_496 0.12 -0.2
562,898 + Pf6N2E2_496 0.12 +1.0
562,899 - Pf6N2E2_496 0.12 +2.2
562,902 + Pf6N2E2_496 0.12 +0.2
562,902 + Pf6N2E2_496 0.12 +1.2
562,902 + Pf6N2E2_496 0.12 -0.6
562,903 - -0.3
562,903 - Pf6N2E2_496 0.12 +1.7
562,903 - Pf6N2E2_496 0.12 +1.9
562,903 - Pf6N2E2_496 0.12 +0.2
562,903 - Pf6N2E2_496 0.12 -1.8
562,903 - Pf6N2E2_496 0.12 -2.5
563,021 + Pf6N2E2_496 0.21 +0.4
563,021 + Pf6N2E2_496 0.21 -2.7
563,022 - Pf6N2E2_496 0.22 -0.3
563,022 - Pf6N2E2_496 0.22 +0.7
563,022 - Pf6N2E2_496 0.22 -0.3
563,036 + Pf6N2E2_496 0.23 +1.0
563,036 + Pf6N2E2_496 0.23 -0.1
563,036 + Pf6N2E2_496 0.23 +0.4
563,522 + Pf6N2E2_496 0.61 -0.8
563,522 + Pf6N2E2_496 0.61 +2.3
563,523 - Pf6N2E2_496 0.61 -0.7
563,523 - Pf6N2E2_496 0.61 +0.1
563,523 - Pf6N2E2_496 0.61 +1.6
563,523 - Pf6N2E2_496 0.61 -2.8
563,523 - Pf6N2E2_496 0.61 -0.6
563,523 - Pf6N2E2_496 0.61 +0.3
563,555 + Pf6N2E2_496 0.63 -2.1
563,555 + Pf6N2E2_496 0.63 -1.3
563,556 - Pf6N2E2_496 0.63 +1.6
563,556 - Pf6N2E2_496 0.63 +0.0

Or see this region's nucleotide sequence