Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_461

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_460 and Pf6N2E2_461 are separated by 290 nucleotidesPf6N2E2_461 and Pf6N2E2_462 are separated by 41 nucleotides Pf6N2E2_460 - Glycerate kinase (EC 2.7.1.31), at 524,208 to 525,344 _460 Pf6N2E2_461 - Methyl-accepting chemotaxis protein, at 525,635 to 527,260 _461 Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486 _462 Position (kb) 525 526 527 528Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 525.006 kb on - strand, within Pf6N2E2_460at 525.207 kb on + strand, within Pf6N2E2_460at 525.207 kb on + strand, within Pf6N2E2_460at 525.207 kb on + strand, within Pf6N2E2_460at 525.208 kb on - strand, within Pf6N2E2_460at 525.361 kb on - strandat 525.361 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.566 kb on - strandat 525.576 kb on - strandat 525.718 kb on - strandat 525.938 kb on + strand, within Pf6N2E2_461at 525.977 kb on + strand, within Pf6N2E2_461at 525.977 kb on + strand, within Pf6N2E2_461at 525.977 kb on + strand, within Pf6N2E2_461at 525.977 kb on + strand, within Pf6N2E2_461at 525.978 kb on - strand, within Pf6N2E2_461at 525.978 kb on - strand, within Pf6N2E2_461at 525.978 kb on - strand, within Pf6N2E2_461at 525.978 kb on - strand, within Pf6N2E2_461at 526.007 kb on + strand, within Pf6N2E2_461at 526.007 kb on + strand, within Pf6N2E2_461at 526.007 kb on + strand, within Pf6N2E2_461at 526.007 kb on + strand, within Pf6N2E2_461at 526.082 kb on - strand, within Pf6N2E2_461at 526.188 kb on - strand, within Pf6N2E2_461at 526.188 kb on - strand, within Pf6N2E2_461at 526.213 kb on + strand, within Pf6N2E2_461at 526.213 kb on + strand, within Pf6N2E2_461at 526.213 kb on + strand, within Pf6N2E2_461at 526.214 kb on - strand, within Pf6N2E2_461at 527.263 kb on + strandat 527.264 kb on - strandat 527.264 kb on - strandat 527.264 kb on - strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.494 kb on + strand, within Pf6N2E2_462at 527.532 kb on + strand, within Pf6N2E2_462at 527.533 kb on - strand, within Pf6N2E2_462at 527.591 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.618 kb on - strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.921 kb on - strand, within Pf6N2E2_462at 527.935 kb on + strand, within Pf6N2E2_462at 527.936 kb on - strand, within Pf6N2E2_462at 527.936 kb on - strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.188 kb on - strand, within Pf6N2E2_462

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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525,006 - Pf6N2E2_460 0.70 -0.8
525,207 + Pf6N2E2_460 0.88 -0.4
525,207 + Pf6N2E2_460 0.88 +0.8
525,207 + Pf6N2E2_460 0.88 -0.6
525,208 - Pf6N2E2_460 0.88 +2.2
525,361 - -1.4
525,361 - -0.7
525,566 - -1.1
525,566 - -0.2
525,566 - -1.2
525,566 - +1.7
525,566 - -2.5
525,566 - -0.4
525,566 - -1.6
525,566 - -0.3
525,566 - +1.0
525,576 - -2.5
525,718 - -0.3
525,938 + Pf6N2E2_461 0.19 -0.3
525,977 + Pf6N2E2_461 0.21 -0.5
525,977 + Pf6N2E2_461 0.21 -0.4
525,977 + Pf6N2E2_461 0.21 -2.8
525,977 + Pf6N2E2_461 0.21 +1.5
525,978 - Pf6N2E2_461 0.21 -2.1
525,978 - Pf6N2E2_461 0.21 +1.0
525,978 - Pf6N2E2_461 0.21 -2.3
525,978 - Pf6N2E2_461 0.21 +0.9
526,007 + Pf6N2E2_461 0.23 +0.6
526,007 + Pf6N2E2_461 0.23 -1.5
526,007 + Pf6N2E2_461 0.23 -2.2
526,007 + Pf6N2E2_461 0.23 -1.9
526,082 - Pf6N2E2_461 0.27 -0.2
526,188 - Pf6N2E2_461 0.34 -1.1
526,188 - Pf6N2E2_461 0.34 -0.5
526,213 + Pf6N2E2_461 0.36 -1.6
526,213 + Pf6N2E2_461 0.36 -1.1
526,213 + Pf6N2E2_461 0.36 +0.9
526,214 - Pf6N2E2_461 0.36 +1.8
527,263 + +0.6
527,264 - -0.1
527,264 - +0.4
527,264 - +0.7
527,299 + +1.3
527,299 + +0.7
527,299 + -0.5
527,299 + +1.7
527,299 + -1.6
527,299 + +1.0
527,299 + +1.9
527,299 + -2.1
527,299 + -1.8
527,299 + -0.1
527,300 - +2.3
527,300 - -0.3
527,300 - +0.5
527,300 - -1.6
527,300 - +1.0
527,300 - -1.6
527,300 - +0.7
527,494 + Pf6N2E2_462 0.16 -1.5
527,532 + Pf6N2E2_462 0.19 +0.9
527,533 - Pf6N2E2_462 0.19 -0.9
527,591 + Pf6N2E2_462 0.24 +0.4
527,617 + Pf6N2E2_462 0.27 +2.7
527,617 + Pf6N2E2_462 0.27 +1.6
527,617 + Pf6N2E2_462 0.27 -3.0
527,618 - Pf6N2E2_462 0.27 +0.7
527,677 + Pf6N2E2_462 0.32 +0.5
527,677 + Pf6N2E2_462 0.32 +1.1
527,677 + Pf6N2E2_462 0.32 +2.1
527,677 + Pf6N2E2_462 0.32 +0.7
527,677 + Pf6N2E2_462 0.32 +1.3
527,677 + Pf6N2E2_462 0.32 -0.6
527,677 + Pf6N2E2_462 0.32 -0.5
527,677 + Pf6N2E2_462 0.32 -1.1
527,678 - Pf6N2E2_462 0.32 +1.1
527,678 - Pf6N2E2_462 0.32 +1.0
527,678 - Pf6N2E2_462 0.32 -0.5
527,678 - Pf6N2E2_462 0.32 -1.8
527,678 - Pf6N2E2_462 0.32 -0.5
527,678 - Pf6N2E2_462 0.32 +1.3
527,678 - Pf6N2E2_462 0.32 -1.1
527,678 - Pf6N2E2_462 0.32 -0.3
527,678 - Pf6N2E2_462 0.32 +1.5
527,686 + Pf6N2E2_462 0.32 +0.0
527,686 + Pf6N2E2_462 0.32 -3.1
527,686 + Pf6N2E2_462 0.32 +0.9
527,686 + Pf6N2E2_462 0.32 +0.8
527,686 + Pf6N2E2_462 0.32 +1.2
527,686 + Pf6N2E2_462 0.32 +1.3
527,686 + Pf6N2E2_462 0.32 +0.2
527,687 - Pf6N2E2_462 0.32 +0.5
527,687 - Pf6N2E2_462 0.32 -2.6
527,687 - Pf6N2E2_462 0.32 +1.2
527,687 - Pf6N2E2_462 0.32 +1.2
527,687 - Pf6N2E2_462 0.32 -3.0
527,687 - Pf6N2E2_462 0.32 +1.7
527,687 - Pf6N2E2_462 0.32 -1.6
527,687 - Pf6N2E2_462 0.32 +1.5
527,921 - Pf6N2E2_462 0.52 -1.5
527,935 + Pf6N2E2_462 0.53 +0.5
527,936 - Pf6N2E2_462 0.54 -0.8
527,936 - Pf6N2E2_462 0.54 +0.2
528,096 + Pf6N2E2_462 0.67 +0.9
528,096 + Pf6N2E2_462 0.67 -0.5
528,096 + Pf6N2E2_462 0.67 -2.8
528,096 + Pf6N2E2_462 0.67 +0.9
528,096 + Pf6N2E2_462 0.67 +0.7
528,097 - Pf6N2E2_462 0.67 +0.4
528,097 - Pf6N2E2_462 0.67 -3.0
528,097 - Pf6N2E2_462 0.67 +0.4
528,097 - Pf6N2E2_462 0.67 -1.1
528,097 - Pf6N2E2_462 0.67 +2.3
528,097 - Pf6N2E2_462 0.67 +0.0
528,097 - Pf6N2E2_462 0.67 +1.0
528,188 - Pf6N2E2_462 0.75 -0.1

Or see this region's nucleotide sequence