Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4490 and Pf6N2E2_4491 are separated by 125 nucleotides Pf6N2E2_4491 and Pf6N2E2_4492 are separated by 144 nucleotides Pf6N2E2_4492 and Pf6N2E2_4493 are separated by 126 nucleotides
Pf6N2E2_4490 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 5,222,272 to 5,223,192
_4490
Pf6N2E2_4491 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 5,223,318 to 5,225,102
_4491
Pf6N2E2_4492 - FIG00953395: hypothetical protein, at 5,225,247 to 5,225,585
_4492
Pf6N2E2_4493 - Transcriptional regulator, AsnC family, at 5,225,712 to 5,226,182
_4493
Position (kb)
5223
5224
5225
5226 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5222.411 kb on + strand, within Pf6N2E2_4490 at 5222.412 kb on - strand, within Pf6N2E2_4490 at 5222.412 kb on - strand, within Pf6N2E2_4490 at 5222.412 kb on - strand, within Pf6N2E2_4490 at 5222.412 kb on - strand, within Pf6N2E2_4490 at 5222.412 kb on - strand, within Pf6N2E2_4490 at 5222.429 kb on + strand, within Pf6N2E2_4490 at 5222.429 kb on + strand, within Pf6N2E2_4490 at 5222.430 kb on - strand, within Pf6N2E2_4490 at 5222.430 kb on - strand, within Pf6N2E2_4490 at 5222.646 kb on + strand, within Pf6N2E2_4490 at 5222.646 kb on + strand, within Pf6N2E2_4490 at 5222.647 kb on - strand, within Pf6N2E2_4490 at 5222.647 kb on - strand, within Pf6N2E2_4490 at 5222.842 kb on - strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.844 kb on + strand, within Pf6N2E2_4490 at 5222.845 kb on - strand, within Pf6N2E2_4490 at 5222.845 kb on - strand, within Pf6N2E2_4490 at 5222.845 kb on - strand, within Pf6N2E2_4490 at 5222.845 kb on - strand, within Pf6N2E2_4490 at 5222.907 kb on + strand, within Pf6N2E2_4490 at 5222.992 kb on + strand, within Pf6N2E2_4490 at 5223.077 kb on + strand, within Pf6N2E2_4490 at 5223.169 kb on + strand at 5223.175 kb on + strand at 5223.175 kb on + strand at 5223.175 kb on + strand at 5223.175 kb on + strand at 5223.175 kb on + strand at 5223.176 kb on - strand at 5223.238 kb on + strand at 5223.239 kb on - strand at 5223.239 kb on - strand at 5223.255 kb on - strand at 5223.255 kb on - strand at 5223.255 kb on - strand at 5223.264 kb on - strand at 5223.319 kb on + strand at 5223.319 kb on + strand at 5223.319 kb on + strand at 5223.319 kb on + strand at 5223.320 kb on - strand at 5223.320 kb on - strand at 5223.320 kb on - strand at 5223.509 kb on - strand, within Pf6N2E2_4491 at 5223.509 kb on - strand, within Pf6N2E2_4491 at 5223.715 kb on + strand, within Pf6N2E2_4491 at 5223.715 kb on + strand, within Pf6N2E2_4491 at 5223.955 kb on - strand, within Pf6N2E2_4491 at 5223.955 kb on - strand, within Pf6N2E2_4491 at 5223.955 kb on - strand, within Pf6N2E2_4491 at 5224.025 kb on - strand, within Pf6N2E2_4491 at 5224.025 kb on - strand, within Pf6N2E2_4491 at 5224.025 kb on - strand, within Pf6N2E2_4491 at 5224.358 kb on - strand, within Pf6N2E2_4491 at 5224.358 kb on - strand, within Pf6N2E2_4491 at 5224.398 kb on - strand, within Pf6N2E2_4491 at 5224.398 kb on - strand, within Pf6N2E2_4491 at 5224.427 kb on + strand, within Pf6N2E2_4491 at 5224.453 kb on + strand, within Pf6N2E2_4491 at 5224.454 kb on - strand, within Pf6N2E2_4491 at 5224.454 kb on - strand, within Pf6N2E2_4491 at 5224.454 kb on - strand, within Pf6N2E2_4491 at 5224.475 kb on + strand, within Pf6N2E2_4491 at 5224.475 kb on + strand, within Pf6N2E2_4491 at 5224.476 kb on - strand, within Pf6N2E2_4491 at 5224.609 kb on + strand, within Pf6N2E2_4491 at 5224.610 kb on - strand, within Pf6N2E2_4491 at 5224.610 kb on - strand, within Pf6N2E2_4491 at 5224.668 kb on + strand, within Pf6N2E2_4491 at 5224.669 kb on - strand, within Pf6N2E2_4491 at 5224.711 kb on + strand, within Pf6N2E2_4491 at 5224.711 kb on + strand, within Pf6N2E2_4491 at 5224.711 kb on + strand, within Pf6N2E2_4491 at 5224.711 kb on + strand, within Pf6N2E2_4491 at 5224.711 kb on + strand, within Pf6N2E2_4491 at 5224.712 kb on - strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.789 kb on + strand, within Pf6N2E2_4491 at 5224.790 kb on - strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.846 kb on + strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5224.847 kb on - strand, within Pf6N2E2_4491 at 5225.112 kb on + strand at 5225.113 kb on - strand at 5225.178 kb on + strand at 5225.179 kb on - strand at 5225.192 kb on + strand at 5225.282 kb on + strand, within Pf6N2E2_4492 at 5225.283 kb on - strand, within Pf6N2E2_4492 at 5225.324 kb on + strand, within Pf6N2E2_4492 at 5225.325 kb on - strand, within Pf6N2E2_4492 at 5225.325 kb on - strand, within Pf6N2E2_4492 at 5225.328 kb on + strand, within Pf6N2E2_4492 at 5225.329 kb on - strand, within Pf6N2E2_4492 at 5225.472 kb on + strand, within Pf6N2E2_4492 at 5225.472 kb on + strand, within Pf6N2E2_4492 at 5225.473 kb on - strand, within Pf6N2E2_4492 at 5225.503 kb on + strand, within Pf6N2E2_4492 at 5225.559 kb on + strand at 5225.634 kb on - strand at 5225.645 kb on - strand at 5225.645 kb on - strand at 5225.687 kb on + strand at 5225.906 kb on + strand, within Pf6N2E2_4493 at 5225.906 kb on + strand, within Pf6N2E2_4493 at 5225.906 kb on + strand, within Pf6N2E2_4493 at 5225.906 kb on + strand, within Pf6N2E2_4493 at 5225.907 kb on - strand, within Pf6N2E2_4493 at 5225.998 kb on + strand, within Pf6N2E2_4493 at 5225.998 kb on + strand, within Pf6N2E2_4493 at 5225.999 kb on - strand, within Pf6N2E2_4493 at 5225.999 kb on - strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.009 kb on + strand, within Pf6N2E2_4493 at 5226.010 kb on - strand, within Pf6N2E2_4493 at 5226.010 kb on - strand, within Pf6N2E2_4493 at 5226.010 kb on - strand, within Pf6N2E2_4493 at 5226.010 kb on - strand, within Pf6N2E2_4493 at 5226.069 kb on + strand, within Pf6N2E2_4493 at 5226.069 kb on + strand, within Pf6N2E2_4493 at 5226.070 kb on - strand, within Pf6N2E2_4493 at 5226.070 kb on - strand, within Pf6N2E2_4493 at 5226.070 kb on - strand, within Pf6N2E2_4493 at 5226.070 kb on - strand, within Pf6N2E2_4493
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 5,222,411 + Pf6N2E2_4490 0.15 -0.6 5,222,412 - Pf6N2E2_4490 0.15 -1.3 5,222,412 - Pf6N2E2_4490 0.15 -1.4 5,222,412 - Pf6N2E2_4490 0.15 -1.6 5,222,412 - Pf6N2E2_4490 0.15 -2.3 5,222,412 - Pf6N2E2_4490 0.15 -0.8 5,222,429 + Pf6N2E2_4490 0.17 +1.1 5,222,429 + Pf6N2E2_4490 0.17 +0.9 5,222,430 - Pf6N2E2_4490 0.17 -1.3 5,222,430 - Pf6N2E2_4490 0.17 -0.5 5,222,646 + Pf6N2E2_4490 0.41 -0.4 5,222,646 + Pf6N2E2_4490 0.41 +0.5 5,222,647 - Pf6N2E2_4490 0.41 -1.7 5,222,647 - Pf6N2E2_4490 0.41 +1.0 5,222,842 - Pf6N2E2_4490 0.62 +0.6 5,222,844 + Pf6N2E2_4490 0.62 -0.5 5,222,844 + Pf6N2E2_4490 0.62 -0.0 5,222,844 + Pf6N2E2_4490 0.62 +1.1 5,222,844 + Pf6N2E2_4490 0.62 +0.4 5,222,844 + Pf6N2E2_4490 0.62 +3.6 5,222,844 + Pf6N2E2_4490 0.62 -3.5 5,222,844 + Pf6N2E2_4490 0.62 +0.3 5,222,844 + Pf6N2E2_4490 0.62 -0.6 5,222,844 + Pf6N2E2_4490 0.62 +0.6 5,222,844 + Pf6N2E2_4490 0.62 -0.1 5,222,845 - Pf6N2E2_4490 0.62 -2.3 5,222,845 - Pf6N2E2_4490 0.62 +1.5 5,222,845 - Pf6N2E2_4490 0.62 +0.7 5,222,845 - Pf6N2E2_4490 0.62 +1.2 5,222,907 + Pf6N2E2_4490 0.69 -0.1 5,222,992 + Pf6N2E2_4490 0.78 +1.6 5,223,077 + Pf6N2E2_4490 0.87 +0.3 5,223,169 + +0.4 5,223,175 + -0.8 5,223,175 + -3.1 5,223,175 + +1.4 5,223,175 + +1.4 5,223,175 + +0.7 5,223,176 - +0.7 5,223,238 + -1.5 5,223,239 - +1.5 5,223,239 - -1.4 5,223,255 - +2.6 5,223,255 - -0.9 5,223,255 - -2.4 5,223,264 - -0.6 5,223,319 + -1.3 5,223,319 + +0.3 5,223,319 + -0.7 5,223,319 + +1.4 5,223,320 - -1.5 5,223,320 - -0.7 5,223,320 - -1.5 5,223,509 - Pf6N2E2_4491 0.11 +1.8 5,223,509 - Pf6N2E2_4491 0.11 -0.4 5,223,715 + Pf6N2E2_4491 0.22 -0.5 5,223,715 + Pf6N2E2_4491 0.22 +0.8 5,223,955 - Pf6N2E2_4491 0.36 +0.2 5,223,955 - Pf6N2E2_4491 0.36 +0.1 5,223,955 - Pf6N2E2_4491 0.36 +0.5 5,224,025 - Pf6N2E2_4491 0.40 -2.9 5,224,025 - Pf6N2E2_4491 0.40 +0.7 5,224,025 - Pf6N2E2_4491 0.40 -1.6 5,224,358 - Pf6N2E2_4491 0.58 +0.7 5,224,358 - Pf6N2E2_4491 0.58 -2.6 5,224,398 - Pf6N2E2_4491 0.61 +1.1 5,224,398 - Pf6N2E2_4491 0.61 -2.3 5,224,427 + Pf6N2E2_4491 0.62 -0.7 5,224,453 + Pf6N2E2_4491 0.64 +1.8 5,224,454 - Pf6N2E2_4491 0.64 +0.0 5,224,454 - Pf6N2E2_4491 0.64 -0.3 5,224,454 - Pf6N2E2_4491 0.64 -0.7 5,224,475 + Pf6N2E2_4491 0.65 -2.3 5,224,475 + Pf6N2E2_4491 0.65 -1.7 5,224,476 - Pf6N2E2_4491 0.65 -1.9 5,224,609 + Pf6N2E2_4491 0.72 -1.4 5,224,610 - Pf6N2E2_4491 0.72 +2.6 5,224,610 - Pf6N2E2_4491 0.72 -0.9 5,224,668 + Pf6N2E2_4491 0.76 +1.7 5,224,669 - Pf6N2E2_4491 0.76 +1.5 5,224,711 + Pf6N2E2_4491 0.78 -1.8 5,224,711 + Pf6N2E2_4491 0.78 +0.8 5,224,711 + Pf6N2E2_4491 0.78 +0.5 5,224,711 + Pf6N2E2_4491 0.78 -0.9 5,224,711 + Pf6N2E2_4491 0.78 -2.5 5,224,712 - Pf6N2E2_4491 0.78 +0.9 5,224,789 + Pf6N2E2_4491 0.82 +1.3 5,224,789 + Pf6N2E2_4491 0.82 +1.5 5,224,789 + Pf6N2E2_4491 0.82 -1.6 5,224,789 + Pf6N2E2_4491 0.82 -0.1 5,224,789 + Pf6N2E2_4491 0.82 -1.0 5,224,789 + Pf6N2E2_4491 0.82 -1.2 5,224,789 + Pf6N2E2_4491 0.82 +0.8 5,224,789 + Pf6N2E2_4491 0.82 -2.0 5,224,790 - Pf6N2E2_4491 0.82 -2.3 5,224,846 + Pf6N2E2_4491 0.86 +5.3 5,224,846 + Pf6N2E2_4491 0.86 +1.1 5,224,846 + Pf6N2E2_4491 0.86 -1.2 5,224,846 + Pf6N2E2_4491 0.86 +1.4 5,224,846 + Pf6N2E2_4491 0.86 -1.0 5,224,846 + Pf6N2E2_4491 0.86 +1.3 5,224,846 + Pf6N2E2_4491 0.86 +0.7 5,224,846 + Pf6N2E2_4491 0.86 +2.3 5,224,846 + Pf6N2E2_4491 0.86 -0.5 5,224,846 + Pf6N2E2_4491 0.86 -1.5 5,224,846 + Pf6N2E2_4491 0.86 +0.7 5,224,846 + Pf6N2E2_4491 0.86 +0.4 5,224,847 - Pf6N2E2_4491 0.86 +0.5 5,224,847 - Pf6N2E2_4491 0.86 +1.5 5,224,847 - Pf6N2E2_4491 0.86 -1.9 5,224,847 - Pf6N2E2_4491 0.86 +1.7 5,224,847 - Pf6N2E2_4491 0.86 -2.5 5,224,847 - Pf6N2E2_4491 0.86 +0.2 5,224,847 - Pf6N2E2_4491 0.86 +0.5 5,224,847 - Pf6N2E2_4491 0.86 +1.0 5,224,847 - Pf6N2E2_4491 0.86 -1.7 5,225,112 + +0.2 5,225,113 - -0.1 5,225,178 + +1.0 5,225,179 - +0.5 5,225,192 + +0.9 5,225,282 + Pf6N2E2_4492 0.10 +1.5 5,225,283 - Pf6N2E2_4492 0.11 +0.7 5,225,324 + Pf6N2E2_4492 0.23 +1.4 5,225,325 - Pf6N2E2_4492 0.23 +0.0 5,225,325 - Pf6N2E2_4492 0.23 +2.3 5,225,328 + Pf6N2E2_4492 0.24 -0.3 5,225,329 - Pf6N2E2_4492 0.24 +0.5 5,225,472 + Pf6N2E2_4492 0.66 +1.4 5,225,472 + Pf6N2E2_4492 0.66 -2.5 5,225,473 - Pf6N2E2_4492 0.67 +0.7 5,225,503 + Pf6N2E2_4492 0.76 -0.4 5,225,559 + -2.7 5,225,634 - -0.8 5,225,645 - +0.8 5,225,645 - +1.1 5,225,687 + -0.5 5,225,906 + Pf6N2E2_4493 0.41 -2.4 5,225,906 + Pf6N2E2_4493 0.41 -0.9 5,225,906 + Pf6N2E2_4493 0.41 +1.3 5,225,906 + Pf6N2E2_4493 0.41 +2.0 5,225,907 - Pf6N2E2_4493 0.41 -1.0 5,225,998 + Pf6N2E2_4493 0.61 -2.4 5,225,998 + Pf6N2E2_4493 0.61 +0.3 5,225,999 - Pf6N2E2_4493 0.61 +0.0 5,225,999 - Pf6N2E2_4493 0.61 -1.7 5,226,009 + Pf6N2E2_4493 0.63 -0.1 5,226,009 + Pf6N2E2_4493 0.63 -2.7 5,226,009 + Pf6N2E2_4493 0.63 +0.2 5,226,009 + Pf6N2E2_4493 0.63 -1.2 5,226,009 + Pf6N2E2_4493 0.63 +0.9 5,226,009 + Pf6N2E2_4493 0.63 +0.2 5,226,010 - Pf6N2E2_4493 0.63 +1.1 5,226,010 - Pf6N2E2_4493 0.63 +1.2 5,226,010 - Pf6N2E2_4493 0.63 +1.5 5,226,010 - Pf6N2E2_4493 0.63 +2.0 5,226,069 + Pf6N2E2_4493 0.76 +0.5 5,226,069 + Pf6N2E2_4493 0.76 +2.1 5,226,070 - Pf6N2E2_4493 0.76 +1.4 5,226,070 - Pf6N2E2_4493 0.76 +2.4 5,226,070 - Pf6N2E2_4493 0.76 +1.2 5,226,070 - Pf6N2E2_4493 0.76 -0.1
Or see this region's nucleotide sequence