Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_437

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_436 and Pf6N2E2_437 are separated by 88 nucleotidesPf6N2E2_437 and Pf6N2E2_438 overlap by 11 nucleotides Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085 _436 Pf6N2E2_437 - Alkylated DNA repair protein AlkB, at 503,174 to 503,851 _437 Pf6N2E2_438 - hypothetical protein, at 503,841 to 504,569 _438 Position (kb) 503 504Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 502.206 kb on - strand, within Pf6N2E2_436at 502.300 kb on + strand, within Pf6N2E2_436at 502.301 kb on - strand, within Pf6N2E2_436at 502.301 kb on - strand, within Pf6N2E2_436at 502.577 kb on + strand, within Pf6N2E2_436at 502.578 kb on - strand, within Pf6N2E2_436at 502.578 kb on - strand, within Pf6N2E2_436at 502.578 kb on - strand, within Pf6N2E2_436at 502.610 kb on + strand, within Pf6N2E2_436at 502.610 kb on + strand, within Pf6N2E2_436at 502.610 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.967 kb on + strand, within Pf6N2E2_436at 502.968 kb on - strand, within Pf6N2E2_436at 502.968 kb on - strand, within Pf6N2E2_436at 502.968 kb on - strand, within Pf6N2E2_436at 502.984 kb on + strandat 502.991 kb on + strandat 502.991 kb on + strandat 502.992 kb on - strandat 503.092 kb on + strandat 503.092 kb on + strandat 503.092 kb on + strandat 503.092 kb on + strandat 503.093 kb on - strandat 503.116 kb on - strandat 503.116 kb on - strandat 503.417 kb on - strand, within Pf6N2E2_437at 503.417 kb on - strand, within Pf6N2E2_437at 503.452 kb on - strand, within Pf6N2E2_437at 503.581 kb on + strand, within Pf6N2E2_437at 503.583 kb on + strand, within Pf6N2E2_437at 503.584 kb on - strand, within Pf6N2E2_437at 503.584 kb on - strand, within Pf6N2E2_437at 503.584 kb on - strand, within Pf6N2E2_437at 503.647 kb on + strand, within Pf6N2E2_437at 503.648 kb on - strand, within Pf6N2E2_437at 503.648 kb on - strand, within Pf6N2E2_437at 503.648 kb on - strand, within Pf6N2E2_437at 503.648 kb on - strand, within Pf6N2E2_437at 503.650 kb on + strand, within Pf6N2E2_437at 503.650 kb on + strand, within Pf6N2E2_437at 503.651 kb on - strand, within Pf6N2E2_437at 503.651 kb on - strand, within Pf6N2E2_437at 503.651 kb on - strand, within Pf6N2E2_437at 503.651 kb on - strand, within Pf6N2E2_437at 503.882 kb on + strandat 504.059 kb on + strand, within Pf6N2E2_438at 504.122 kb on - strand, within Pf6N2E2_438at 504.145 kb on + strand, within Pf6N2E2_438at 504.146 kb on - strand, within Pf6N2E2_438at 504.146 kb on - strand, within Pf6N2E2_438at 504.161 kb on - strand, within Pf6N2E2_438at 504.299 kb on - strand, within Pf6N2E2_438at 504.355 kb on + strand, within Pf6N2E2_438at 504.528 kb on + strandat 504.528 kb on + strandat 504.529 kb on - strandat 504.529 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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502,206 - Pf6N2E2_436 0.18 +0.4
502,300 + Pf6N2E2_436 0.27 -1.0
502,301 - Pf6N2E2_436 0.27 -2.2
502,301 - Pf6N2E2_436 0.27 -0.2
502,577 + Pf6N2E2_436 0.53 +1.9
502,578 - Pf6N2E2_436 0.53 +0.6
502,578 - Pf6N2E2_436 0.53 +1.7
502,578 - Pf6N2E2_436 0.53 -2.6
502,610 + Pf6N2E2_436 0.56 +0.4
502,610 + Pf6N2E2_436 0.56 +1.5
502,610 + Pf6N2E2_436 0.56 -0.3
502,967 + Pf6N2E2_436 0.89 +2.0
502,967 + Pf6N2E2_436 0.89 +0.8
502,967 + Pf6N2E2_436 0.89 -1.3
502,967 + Pf6N2E2_436 0.89 -0.6
502,967 + Pf6N2E2_436 0.89 -1.1
502,967 + Pf6N2E2_436 0.89 -0.7
502,967 + Pf6N2E2_436 0.89 -0.2
502,968 - Pf6N2E2_436 0.89 -2.8
502,968 - Pf6N2E2_436 0.89 -0.1
502,968 - Pf6N2E2_436 0.89 -1.3
502,984 + -0.9
502,991 + -0.1
502,991 + +1.0
502,992 - +0.3
503,092 + +1.4
503,092 + +0.2
503,092 + -0.4
503,092 + -0.9
503,093 - -0.6
503,116 - +0.5
503,116 - +1.4
503,417 - Pf6N2E2_437 0.36 -2.1
503,417 - Pf6N2E2_437 0.36 -1.4
503,452 - Pf6N2E2_437 0.41 -0.8
503,581 + Pf6N2E2_437 0.60 +2.2
503,583 + Pf6N2E2_437 0.60 -2.6
503,584 - Pf6N2E2_437 0.60 -2.0
503,584 - Pf6N2E2_437 0.60 -0.2
503,584 - Pf6N2E2_437 0.60 +0.2
503,647 + Pf6N2E2_437 0.70 +0.4
503,648 - Pf6N2E2_437 0.70 -1.1
503,648 - Pf6N2E2_437 0.70 -2.4
503,648 - Pf6N2E2_437 0.70 +1.8
503,648 - Pf6N2E2_437 0.70 -1.4
503,650 + Pf6N2E2_437 0.70 +0.3
503,650 + Pf6N2E2_437 0.70 +0.3
503,651 - Pf6N2E2_437 0.70 -2.0
503,651 - Pf6N2E2_437 0.70 +0.2
503,651 - Pf6N2E2_437 0.70 +0.6
503,651 - Pf6N2E2_437 0.70 -1.3
503,882 + +0.9
504,059 + Pf6N2E2_438 0.30 -2.4
504,122 - Pf6N2E2_438 0.39 -0.9
504,145 + Pf6N2E2_438 0.42 -1.6
504,146 - Pf6N2E2_438 0.42 -2.1
504,146 - Pf6N2E2_438 0.42 -1.4
504,161 - Pf6N2E2_438 0.44 +0.5
504,299 - Pf6N2E2_438 0.63 -0.9
504,355 + Pf6N2E2_438 0.71 -1.5
504,528 + +0.2
504,528 + -2.3
504,529 - +1.4
504,529 - +0.4

Or see this region's nucleotide sequence