Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4332 and Pf6N2E2_4333 are separated by 81 nucleotides Pf6N2E2_4333 and Pf6N2E2_4334 are separated by 220 nucleotides Pf6N2E2_4334 and Pf6N2E2_4335 overlap by 4 nucleotides Pf6N2E2_4335 and Pf6N2E2_4336 are separated by 81 nucleotides
Pf6N2E2_4332 - hypothetical protein, at 5,054,927 to 5,055,382
_4332
Pf6N2E2_4333 - Uncharacterized protein ImpD, at 5,055,464 to 5,055,952
_4333
Pf6N2E2_4334 - Uncharacterized protein ImpC, at 5,056,173 to 5,057,675
_4334
Pf6N2E2_4335 - Uncharacterized protein ImpB, at 5,057,672 to 5,058,178
_4335
Pf6N2E2_4336 - Uncharacterized protein ImpA, at 5,058,260 to 5,059,303
_4336
Position (kb) 5056
5057
5058 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.219 kb on + strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.220 kb on - strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.253 kb on + strand, within Pf6N2E2_4332 at 5055.254 kb on - strand, within Pf6N2E2_4332 at 5055.325 kb on - strand, within Pf6N2E2_4332 at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.345 kb on + strand at 5055.346 kb on - strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.391 kb on + strand at 5055.392 kb on - strand at 5055.392 kb on - strand at 5055.465 kb on + strand at 5055.465 kb on + strand at 5055.466 kb on - strand at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.631 kb on + strand, within Pf6N2E2_4333 at 5055.632 kb on - strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.663 kb on + strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.664 kb on - strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.771 kb on + strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.772 kb on - strand, within Pf6N2E2_4333 at 5055.940 kb on + strand at 5055.975 kb on - strand at 5056.175 kb on - strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.240 kb on + strand at 5056.241 kb on - strand at 5056.255 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.270 kb on + strand at 5056.271 kb on - strand at 5056.271 kb on - strand at 5056.271 kb on - strand at 5056.273 kb on + strand at 5056.375 kb on + strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.376 kb on - strand, within Pf6N2E2_4334 at 5056.462 kb on + strand, within Pf6N2E2_4334 at 5056.462 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.492 kb on + strand, within Pf6N2E2_4334 at 5056.493 kb on - strand, within Pf6N2E2_4334 at 5056.544 kb on - strand, within Pf6N2E2_4334 at 5056.819 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334 at 5056.840 kb on + strand, within Pf6N2E2_4334 at 5056.996 kb on + strand, within Pf6N2E2_4334 at 5057.167 kb on + strand, within Pf6N2E2_4334 at 5057.167 kb on + strand, within Pf6N2E2_4334 at 5057.167 kb on + strand, within Pf6N2E2_4334 at 5057.168 kb on - strand, within Pf6N2E2_4334 at 5057.171 kb on - strand, within Pf6N2E2_4334 at 5057.192 kb on - strand, within Pf6N2E2_4334 at 5057.227 kb on + strand, within Pf6N2E2_4334 at 5057.227 kb on + strand, within Pf6N2E2_4334 at 5057.227 kb on + strand, within Pf6N2E2_4334 at 5057.228 kb on - strand, within Pf6N2E2_4334 at 5057.228 kb on - strand, within Pf6N2E2_4334 at 5057.362 kb on + strand, within Pf6N2E2_4334 at 5057.363 kb on - strand, within Pf6N2E2_4334 at 5057.657 kb on + strand at 5057.657 kb on + strand at 5057.657 kb on + strand at 5057.658 kb on - strand at 5057.658 kb on - strand at 5057.658 kb on - strand at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.778 kb on + strand, within Pf6N2E2_4335 at 5057.779 kb on - strand, within Pf6N2E2_4335 at 5057.779 kb on - strand, within Pf6N2E2_4335 at 5057.779 kb on - strand, within Pf6N2E2_4335 at 5057.779 kb on - strand, within Pf6N2E2_4335 at 5057.779 kb on - strand, within Pf6N2E2_4335 at 5058.056 kb on + strand, within Pf6N2E2_4335 at 5058.099 kb on + strand, within Pf6N2E2_4335 at 5058.261 kb on + strand at 5058.262 kb on - strand at 5058.408 kb on + strand, within Pf6N2E2_4336 at 5058.408 kb on + strand, within Pf6N2E2_4336 at 5058.408 kb on + strand, within Pf6N2E2_4336 at 5058.408 kb on + strand, within Pf6N2E2_4336 at 5058.409 kb on - strand, within Pf6N2E2_4336 at 5058.409 kb on - strand, within Pf6N2E2_4336 at 5058.409 kb on - strand, within Pf6N2E2_4336 at 5058.409 kb on - strand, within Pf6N2E2_4336 at 5058.409 kb on - strand, within Pf6N2E2_4336 at 5058.411 kb on + strand, within Pf6N2E2_4336 at 5058.412 kb on - strand, within Pf6N2E2_4336 at 5058.412 kb on - strand, within Pf6N2E2_4336 at 5058.412 kb on - strand, within Pf6N2E2_4336 at 5058.487 kb on + strand, within Pf6N2E2_4336 at 5058.487 kb on + strand, within Pf6N2E2_4336
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 5,055,219 + Pf6N2E2_4332 0.64 +1.0 5,055,219 + Pf6N2E2_4332 0.64 +1.4 5,055,220 - Pf6N2E2_4332 0.64 +2.4 5,055,220 - Pf6N2E2_4332 0.64 +0.9 5,055,253 + Pf6N2E2_4332 0.71 +0.6 5,055,253 + Pf6N2E2_4332 0.71 +0.5 5,055,254 - Pf6N2E2_4332 0.72 +0.1 5,055,325 - Pf6N2E2_4332 0.87 -1.9 5,055,345 + -0.4 5,055,345 + +0.4 5,055,345 + +0.3 5,055,345 + +1.4 5,055,346 - +2.5 5,055,391 + -1.3 5,055,391 + -1.2 5,055,391 + +1.1 5,055,392 - -0.8 5,055,392 - -2.8 5,055,465 + +0.5 5,055,465 + +1.7 5,055,466 - +0.8 5,055,631 + Pf6N2E2_4333 0.34 +1.9 5,055,631 + Pf6N2E2_4333 0.34 -2.2 5,055,632 - Pf6N2E2_4333 0.34 +0.8 5,055,663 + Pf6N2E2_4333 0.41 +1.1 5,055,663 + Pf6N2E2_4333 0.41 -0.1 5,055,663 + Pf6N2E2_4333 0.41 -2.4 5,055,664 - Pf6N2E2_4333 0.41 +1.5 5,055,664 - Pf6N2E2_4333 0.41 +0.1 5,055,771 + Pf6N2E2_4333 0.63 +2.6 5,055,771 + Pf6N2E2_4333 0.63 +0.1 5,055,771 + Pf6N2E2_4333 0.63 +2.1 5,055,772 - Pf6N2E2_4333 0.63 +0.7 5,055,772 - Pf6N2E2_4333 0.63 +1.8 5,055,772 - Pf6N2E2_4333 0.63 -0.5 5,055,772 - Pf6N2E2_4333 0.63 +0.5 5,055,772 - Pf6N2E2_4333 0.63 +0.1 5,055,772 - Pf6N2E2_4333 0.63 +0.9 5,055,772 - Pf6N2E2_4333 0.63 -1.5 5,055,940 + -0.7 5,055,975 - +0.6 5,056,175 - -0.3 5,056,240 + +1.9 5,056,240 + +0.2 5,056,240 + -1.1 5,056,240 + -0.0 5,056,241 - -2.3 5,056,255 + -1.9 5,056,270 + +1.1 5,056,270 + +1.7 5,056,270 + +1.4 5,056,270 + -1.2 5,056,271 - +0.9 5,056,271 - +0.9 5,056,271 - +1.0 5,056,273 + +2.1 5,056,375 + Pf6N2E2_4334 0.13 +1.3 5,056,376 - Pf6N2E2_4334 0.14 +1.0 5,056,376 - Pf6N2E2_4334 0.14 -1.2 5,056,376 - Pf6N2E2_4334 0.14 -3.3 5,056,376 - Pf6N2E2_4334 0.14 -2.8 5,056,462 + Pf6N2E2_4334 0.19 -0.7 5,056,462 + Pf6N2E2_4334 0.19 +0.2 5,056,492 + Pf6N2E2_4334 0.21 +1.2 5,056,492 + Pf6N2E2_4334 0.21 +2.5 5,056,492 + Pf6N2E2_4334 0.21 +3.5 5,056,493 - Pf6N2E2_4334 0.21 +1.0 5,056,544 - Pf6N2E2_4334 0.25 +1.0 5,056,819 + Pf6N2E2_4334 0.43 +1.8 5,056,840 + Pf6N2E2_4334 0.44 +1.1 5,056,840 + Pf6N2E2_4334 0.44 -0.8 5,056,840 + Pf6N2E2_4334 0.44 -1.4 5,056,996 + Pf6N2E2_4334 0.55 +1.9 5,057,167 + Pf6N2E2_4334 0.66 +2.4 5,057,167 + Pf6N2E2_4334 0.66 -0.6 5,057,167 + Pf6N2E2_4334 0.66 -0.5 5,057,168 - Pf6N2E2_4334 0.66 -1.9 5,057,171 - Pf6N2E2_4334 0.66 +1.3 5,057,192 - Pf6N2E2_4334 0.68 +2.7 5,057,227 + Pf6N2E2_4334 0.70 -1.6 5,057,227 + Pf6N2E2_4334 0.70 -1.3 5,057,227 + Pf6N2E2_4334 0.70 +2.4 5,057,228 - Pf6N2E2_4334 0.70 -1.1 5,057,228 - Pf6N2E2_4334 0.70 -0.4 5,057,362 + Pf6N2E2_4334 0.79 -0.9 5,057,363 - Pf6N2E2_4334 0.79 +2.6 5,057,657 + +0.1 5,057,657 + +0.8 5,057,657 + +0.1 5,057,658 - +1.0 5,057,658 - +0.9 5,057,658 - -0.8 5,057,778 + Pf6N2E2_4335 0.21 +0.7 5,057,778 + Pf6N2E2_4335 0.21 -2.7 5,057,778 + Pf6N2E2_4335 0.21 -1.2 5,057,778 + Pf6N2E2_4335 0.21 -1.9 5,057,778 + Pf6N2E2_4335 0.21 +0.7 5,057,778 + Pf6N2E2_4335 0.21 -2.2 5,057,778 + Pf6N2E2_4335 0.21 -0.7 5,057,778 + Pf6N2E2_4335 0.21 +1.0 5,057,778 + Pf6N2E2_4335 0.21 +0.1 5,057,779 - Pf6N2E2_4335 0.21 +0.4 5,057,779 - Pf6N2E2_4335 0.21 +2.6 5,057,779 - Pf6N2E2_4335 0.21 -0.1 5,057,779 - Pf6N2E2_4335 0.21 -2.5 5,057,779 - Pf6N2E2_4335 0.21 +1.3 5,058,056 + Pf6N2E2_4335 0.76 -1.7 5,058,099 + Pf6N2E2_4335 0.84 -0.1 5,058,261 + -2.2 5,058,262 - +1.2 5,058,408 + Pf6N2E2_4336 0.14 +0.7 5,058,408 + Pf6N2E2_4336 0.14 +1.0 5,058,408 + Pf6N2E2_4336 0.14 +0.7 5,058,408 + Pf6N2E2_4336 0.14 +0.1 5,058,409 - Pf6N2E2_4336 0.14 +0.2 5,058,409 - Pf6N2E2_4336 0.14 -0.4 5,058,409 - Pf6N2E2_4336 0.14 -0.8 5,058,409 - Pf6N2E2_4336 0.14 +1.7 5,058,409 - Pf6N2E2_4336 0.14 -1.5 5,058,411 + Pf6N2E2_4336 0.14 +1.3 5,058,412 - Pf6N2E2_4336 0.15 +0.3 5,058,412 - Pf6N2E2_4336 0.15 -0.4 5,058,412 - Pf6N2E2_4336 0.15 -1.2 5,058,487 + Pf6N2E2_4336 0.22 -1.7 5,058,487 + Pf6N2E2_4336 0.22 -1.4
Or see this region's nucleotide sequence