Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4213 and Pf6N2E2_4214 are separated by 45 nucleotides Pf6N2E2_4214 and Pf6N2E2_4215 are separated by 115 nucleotides Pf6N2E2_4215 and Pf6N2E2_4216 are separated by 209 nucleotides Pf6N2E2_4216 and Pf6N2E2_4217 are separated by 1 nucleotides
Pf6N2E2_4213 - ATP synthase beta chain (EC 3.6.3.14), at 4,903,679 to 4,905,055
_4213
Pf6N2E2_4214 - ATP synthase epsilon chain (EC 3.6.3.14), at 4,905,101 to 4,905,526
_4214
Pf6N2E2_4215 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 4,905,642 to 4,907,009
_4215
Pf6N2E2_4216 - Transcriptional regulator of glmS gene, DeoR family, at 4,907,219 to 4,907,995
_4216
Pf6N2E2_4217 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 4,907,997 to 4,909,829
_4217
Position (kb)
4905
4906
4907
4908 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4907.013 kb on + strand at 4907.059 kb on + strand at 4907.067 kb on - strand at 4907.708 kb on + strand, within Pf6N2E2_4216 at 4907.761 kb on + strand, within Pf6N2E2_4216 at 4907.761 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.959 kb on + strand at 4907.959 kb on + strand at 4907.959 kb on + strand at 4907.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 4,907,013 + -1.7 4,907,059 + +0.2 4,907,067 - +0.8 4,907,708 + Pf6N2E2_4216 0.63 -0.5 4,907,761 + Pf6N2E2_4216 0.70 +0.3 4,907,761 + Pf6N2E2_4216 0.70 -0.3 4,907,823 + Pf6N2E2_4216 0.78 -0.9 4,907,823 + Pf6N2E2_4216 0.78 +0.8 4,907,823 + Pf6N2E2_4216 0.78 +0.4 4,907,959 + +3.3 4,907,959 + +1.0 4,907,959 + +2.4 4,907,959 + -1.9
Or see this region's nucleotide sequence