Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4202 and Pf6N2E2_4203 are separated by 565 nucleotides Pf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotides Pf6N2E2_4204 and Pf6N2E2_4205 are separated by 18 nucleotides
Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462
_4202
Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926
_4203
Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567
_4204
Pf6N2E2_4205 - Chromosome (plasmid) partitioning protein ParA, at 4,896,586 to 4,897,383
_4205
Position (kb)
4894
4895
4896 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4893.460 kb on + strand at 4893.623 kb on + strand at 4893.710 kb on + strand at 4893.710 kb on + strand at 4893.710 kb on + strand at 4893.711 kb on - strand at 4893.711 kb on - strand at 4893.711 kb on - strand at 4893.767 kb on - strand at 4893.767 kb on - strand at 4893.832 kb on - strand at 4893.941 kb on + strand at 4893.941 kb on + strand at 4893.941 kb on + strand at 4893.941 kb on + strand at 4894.007 kb on + strand at 4894.007 kb on + strand at 4894.007 kb on + strand at 4896.059 kb on - strand, within Pf6N2E2_4204 at 4896.059 kb on - strand, within Pf6N2E2_4204 at 4896.304 kb on - strand, within Pf6N2E2_4204 at 4896.549 kb on - strand at 4896.592 kb on - strand at 4896.597 kb on - strand at 4896.597 kb on - strand at 4896.646 kb on - strand at 4896.766 kb on + strand, within Pf6N2E2_4205 at 4896.767 kb on - strand, within Pf6N2E2_4205
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 4,893,460 + -0.7 4,893,623 + +0.2 4,893,710 + +2.0 4,893,710 + -2.7 4,893,710 + +0.6 4,893,711 - +0.8 4,893,711 - -2.9 4,893,711 - -0.2 4,893,767 - -0.8 4,893,767 - +0.9 4,893,832 - -1.0 4,893,941 + -1.0 4,893,941 + +0.9 4,893,941 + -1.6 4,893,941 + -2.3 4,894,007 + +0.5 4,894,007 + +0.3 4,894,007 + +0.6 4,896,059 - Pf6N2E2_4204 0.20 -0.5 4,896,059 - Pf6N2E2_4204 0.20 +0.7 4,896,304 - Pf6N2E2_4204 0.58 -1.2 4,896,549 - -0.5 4,896,592 - -2.0 4,896,597 - +1.1 4,896,597 - -0.3 4,896,646 - +1.5 4,896,766 + Pf6N2E2_4205 0.23 +0.5 4,896,767 - Pf6N2E2_4205 0.23 -1.4
Or see this region's nucleotide sequence