Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4203

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4202 and Pf6N2E2_4203 are separated by 565 nucleotidesPf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotidesPf6N2E2_4204 and Pf6N2E2_4205 are separated by 18 nucleotides Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462 _4202 Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926 _4203 Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567 _4204 Pf6N2E2_4205 - Chromosome (plasmid) partitioning protein ParA, at 4,896,586 to 4,897,383 _4205 Position (kb) 4894 4895 4896Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4893.460 kb on + strandat 4893.623 kb on + strandat 4893.710 kb on + strandat 4893.710 kb on + strandat 4893.710 kb on + strandat 4893.711 kb on - strandat 4893.711 kb on - strandat 4893.711 kb on - strandat 4893.767 kb on - strandat 4893.767 kb on - strandat 4893.832 kb on - strandat 4893.941 kb on + strandat 4893.941 kb on + strandat 4893.941 kb on + strandat 4893.941 kb on + strandat 4894.007 kb on + strandat 4894.007 kb on + strandat 4894.007 kb on + strandat 4896.059 kb on - strand, within Pf6N2E2_4204at 4896.059 kb on - strand, within Pf6N2E2_4204at 4896.304 kb on - strand, within Pf6N2E2_4204at 4896.549 kb on - strandat 4896.592 kb on - strandat 4896.597 kb on - strandat 4896.597 kb on - strandat 4896.646 kb on - strandat 4896.766 kb on + strand, within Pf6N2E2_4205at 4896.767 kb on - strand, within Pf6N2E2_4205

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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4,893,460 + -0.7
4,893,623 + +0.2
4,893,710 + +2.0
4,893,710 + -2.7
4,893,710 + +0.6
4,893,711 - +0.8
4,893,711 - -2.9
4,893,711 - -0.2
4,893,767 - -0.8
4,893,767 - +0.9
4,893,832 - -1.0
4,893,941 + -1.0
4,893,941 + +0.9
4,893,941 + -1.6
4,893,941 + -2.3
4,894,007 + +0.5
4,894,007 + +0.3
4,894,007 + +0.6
4,896,059 - Pf6N2E2_4204 0.20 -0.5
4,896,059 - Pf6N2E2_4204 0.20 +0.7
4,896,304 - Pf6N2E2_4204 0.58 -1.2
4,896,549 - -0.5
4,896,592 - -2.0
4,896,597 - +1.1
4,896,597 - -0.3
4,896,646 - +1.5
4,896,766 + Pf6N2E2_4205 0.23 +0.5
4,896,767 - Pf6N2E2_4205 0.23 -1.4

Or see this region's nucleotide sequence