Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4201

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4200 and Pf6N2E2_4201 are separated by 240 nucleotidesPf6N2E2_4201 and Pf6N2E2_4202 are separated by 79 nucleotides Pf6N2E2_4200 - Ribonuclease P protein component (EC 3.1.26.5), at 4,889,892 to 4,890,089 _4200 Pf6N2E2_4201 - Inner membrane protein translocase component YidC, long form, at 4,890,330 to 4,892,012 _4201 Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462 _4202 Position (kb) 4890 4891 4892 4893Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4889.332 kb on + strandat 4889.358 kb on + strandat 4889.371 kb on + strandat 4889.371 kb on + strandat 4889.377 kb on + strandat 4889.377 kb on + strandat 4889.377 kb on + strandat 4889.377 kb on + strandat 4889.377 kb on + strandat 4889.379 kb on + strandat 4889.383 kb on + strandat 4889.383 kb on + strandat 4889.383 kb on + strandat 4889.383 kb on + strandat 4889.422 kb on + strandat 4889.422 kb on + strandat 4889.422 kb on + strandat 4889.422 kb on + strandat 4889.422 kb on + strandat 4890.070 kb on + strandat 4890.087 kb on + strandat 4890.115 kb on + strandat 4890.297 kb on + strandat 4891.976 kb on + strandat 4892.046 kb on + strandat 4892.091 kb on + strandat 4892.091 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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4,889,332 + -2.5
4,889,358 + -0.1
4,889,371 + -2.7
4,889,371 + +1.8
4,889,377 + +1.2
4,889,377 + -0.9
4,889,377 + -1.8
4,889,377 + -2.1
4,889,377 + -1.7
4,889,379 + -0.9
4,889,383 + +1.5
4,889,383 + +0.4
4,889,383 + -3.0
4,889,383 + +2.5
4,889,422 + +1.3
4,889,422 + -1.9
4,889,422 + -3.0
4,889,422 + -1.5
4,889,422 + +2.0
4,890,070 + -0.6
4,890,087 + -1.2
4,890,115 + +1.2
4,890,297 + +0.9
4,891,976 + -2.5
4,892,046 + -3.6
4,892,091 + -0.7
4,892,091 + +0.1

Or see this region's nucleotide sequence