Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3996 and Pf6N2E2_3997 are separated by 190 nucleotides Pf6N2E2_3997 and Pf6N2E2_3998 are separated by 88 nucleotides Pf6N2E2_3998 and Pf6N2E2_3999 are separated by 60 nucleotides
Pf6N2E2_3996 - Transcriptional regulator, AraC family, at 4,666,212 to 4,667,216
_3996
Pf6N2E2_3997 - transcriptional regulator, Crp/Fnr family, at 4,667,407 to 4,668,093
_3997
Pf6N2E2_3998 - FIG028593: membrane protein, at 4,668,182 to 4,668,706
_3998
Pf6N2E2_3999 - Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site, at 4,668,767 to 4,670,989
_3999
Position (kb)
4667
4668
4669 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.687 kb on - strand, within Pf6N2E2_3996 at 4666.704 kb on + strand, within Pf6N2E2_3996 at 4666.705 kb on - strand, within Pf6N2E2_3996 at 4666.878 kb on - strand, within Pf6N2E2_3996 at 4666.919 kb on - strand, within Pf6N2E2_3996 at 4666.919 kb on - strand, within Pf6N2E2_3996 at 4667.121 kb on + strand at 4667.122 kb on - strand at 4667.122 kb on - strand at 4667.335 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.338 kb on - strand at 4667.724 kb on - strand, within Pf6N2E2_3997 at 4667.868 kb on + strand, within Pf6N2E2_3997 at 4667.868 kb on + strand, within Pf6N2E2_3997 at 4667.869 kb on - strand, within Pf6N2E2_3997 at 4667.936 kb on + strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.025 kb on + strand at 4668.025 kb on + strand at 4668.026 kb on - strand at 4668.026 kb on - strand at 4668.026 kb on - strand at 4668.047 kb on + strand at 4668.048 kb on - strand at 4668.048 kb on - strand at 4668.048 kb on - strand at 4668.212 kb on + strand at 4668.213 kb on - strand at 4668.221 kb on + strand at 4668.222 kb on - strand at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.375 kb on - strand, within Pf6N2E2_3998 at 4668.375 kb on - strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.379 kb on - strand at 4668.379 kb on - strand, within Pf6N2E2_3998 at 4668.533 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.621 kb on + strand, within Pf6N2E2_3998 at 4668.769 kb on - strand at 4668.811 kb on + strand at 4668.978 kb on + strand at 4668.978 kb on + strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4669.049 kb on + strand, within Pf6N2E2_3999 at 4669.050 kb on - strand, within Pf6N2E2_3999
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 4,666,651 - Pf6N2E2_3996 0.44 +1.0 4,666,651 - Pf6N2E2_3996 0.44 +0.3 4,666,651 - Pf6N2E2_3996 0.44 -0.0 4,666,687 - Pf6N2E2_3996 0.47 +0.9 4,666,704 + Pf6N2E2_3996 0.49 +0.5 4,666,705 - Pf6N2E2_3996 0.49 +0.1 4,666,878 - Pf6N2E2_3996 0.66 +1.4 4,666,919 - Pf6N2E2_3996 0.70 +0.1 4,666,919 - Pf6N2E2_3996 0.70 -1.2 4,667,121 + -1.2 4,667,122 - -0.2 4,667,122 - -1.4 4,667,335 + -1.7 4,667,337 + -1.9 4,667,337 + +0.2 4,667,337 + +1.4 4,667,337 + -1.1 4,667,338 - -0.5 4,667,724 - Pf6N2E2_3997 0.46 -0.6 4,667,868 + Pf6N2E2_3997 0.67 -3.4 4,667,868 + Pf6N2E2_3997 0.67 +0.4 4,667,869 - Pf6N2E2_3997 0.67 -2.6 4,667,936 + Pf6N2E2_3997 0.77 -0.1 4,667,937 - Pf6N2E2_3997 0.77 -1.9 4,667,937 - Pf6N2E2_3997 0.77 +0.1 4,667,937 - Pf6N2E2_3997 0.77 -3.2 4,668,019 - Pf6N2E2_3997 0.89 -1.2 4,668,019 - Pf6N2E2_3997 0.89 -1.2 4,668,019 - Pf6N2E2_3997 0.89 -0.6 4,668,019 - Pf6N2E2_3997 0.89 -2.5 4,668,019 - Pf6N2E2_3997 0.89 -0.1 4,668,025 + -0.8 4,668,025 + -1.4 4,668,026 - -1.4 4,668,026 - +2.4 4,668,026 - +2.2 4,668,047 + +0.9 4,668,048 - -0.7 4,668,048 - -2.7 4,668,048 - +1.2 4,668,212 + -1.5 4,668,213 - +1.7 4,668,221 + +0.9 4,668,222 - +0.1 4,668,374 + Pf6N2E2_3998 0.37 -0.5 4,668,374 + Pf6N2E2_3998 0.37 +0.4 4,668,374 + Pf6N2E2_3998 0.37 +0.2 4,668,375 - Pf6N2E2_3998 0.37 +0.4 4,668,375 - Pf6N2E2_3998 0.37 +1.1 4,668,378 + Pf6N2E2_3998 0.37 +0.8 4,668,378 + Pf6N2E2_3998 0.37 +0.2 4,668,378 + Pf6N2E2_3998 0.37 +1.3 4,668,379 - +2.4 4,668,379 - Pf6N2E2_3998 0.38 +0.0 4,668,533 + Pf6N2E2_3998 0.67 +0.6 4,668,536 + Pf6N2E2_3998 0.67 -0.3 4,668,536 + Pf6N2E2_3998 0.67 +3.1 4,668,536 + Pf6N2E2_3998 0.67 +0.5 4,668,536 + Pf6N2E2_3998 0.67 +1.5 4,668,537 - Pf6N2E2_3998 0.68 +0.4 4,668,537 - Pf6N2E2_3998 0.68 +1.4 4,668,537 - Pf6N2E2_3998 0.68 +3.0 4,668,537 - Pf6N2E2_3998 0.68 -0.1 4,668,621 + Pf6N2E2_3998 0.84 -2.2 4,668,769 - +1.3 4,668,811 + -2.2 4,668,978 + +1.3 4,668,978 + -1.8 4,668,979 - +1.1 4,668,979 - -0.6 4,668,979 - -1.3 4,668,979 - +0.5 4,668,979 - -1.3 4,668,979 - -0.5 4,668,979 - -2.2 4,668,979 - -0.7 4,669,049 + Pf6N2E2_3999 0.13 +1.6 4,669,050 - Pf6N2E2_3999 0.13 -2.0
Or see this region's nucleotide sequence