Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_395 and Pf6N2E2_396 are separated by 684 nucleotides Pf6N2E2_396 and Pf6N2E2_397 are separated by 162 nucleotides
Pf6N2E2_395 - Glutathione S-transferase (EC 2.5.1.18), at 463,397 to 463,828
_395
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Pf6N2E2_397 - Large exoproteins involved in heme utilization or adhesion, at 465,083 to 467,020
_397
Position (kb)
464
465 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 463.558 kb on + strand, within Pf6N2E2_395 at 463.602 kb on + strand, within Pf6N2E2_395 at 463.603 kb on - strand, within Pf6N2E2_395 at 463.697 kb on + strand, within Pf6N2E2_395 at 463.698 kb on - strand, within Pf6N2E2_395 at 463.731 kb on - strand, within Pf6N2E2_395 at 463.795 kb on - strand at 463.795 kb on - strand at 463.882 kb on - strand at 463.924 kb on - strand at 463.957 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.846 kb on + strand, within Pf6N2E2_396 at 464.857 kb on + strand, within Pf6N2E2_396 at 464.900 kb on + strand at 464.900 kb on + strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.913 kb on + strand at 464.913 kb on + strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.930 kb on + strand at 465.034 kb on + strand at 465.035 kb on - strand at 465.084 kb on + strand at 465.084 kb on + strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.225 kb on + strand at 465.225 kb on + strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.348 kb on + strand, within Pf6N2E2_397 at 465.391 kb on - strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.551 kb on + strand, within Pf6N2E2_397 at 465.552 kb on - strand, within Pf6N2E2_397 at 465.691 kb on - strand, within Pf6N2E2_397 at 465.820 kb on + strand, within Pf6N2E2_397 at 465.843 kb on + strand, within Pf6N2E2_397 at 465.874 kb on + strand, within Pf6N2E2_397 at 465.875 kb on - strand, within Pf6N2E2_397
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 463,558 + Pf6N2E2_395 0.37 +1.2 463,602 + Pf6N2E2_395 0.47 +0.2 463,603 - Pf6N2E2_395 0.48 -2.1 463,697 + Pf6N2E2_395 0.69 -0.8 463,698 - Pf6N2E2_395 0.70 +0.5 463,731 - Pf6N2E2_395 0.77 -0.0 463,795 - -2.0 463,795 - -1.1 463,882 - +1.9 463,924 - -2.3 463,957 - -1.8 464,009 - +0.1 464,009 - -1.3 464,475 + +0.3 464,476 - -0.7 464,508 - +0.1 464,508 - +0.4 464,508 - -2.9 464,508 - -1.3 464,511 + -1.9 464,511 + -0.3 464,511 + +0.7 464,511 + -0.6 464,512 - -0.2 464,512 - +0.1 464,532 + +2.2 464,532 + -1.3 464,533 - +0.3 464,533 - +1.4 464,594 - Pf6N2E2_396 0.20 -0.0 464,594 - Pf6N2E2_396 0.20 +0.1 464,594 - Pf6N2E2_396 0.20 -0.6 464,690 + Pf6N2E2_396 0.43 -0.6 464,719 - Pf6N2E2_396 0.50 -2.9 464,719 - Pf6N2E2_396 0.50 -2.4 464,812 + Pf6N2E2_396 0.73 -0.6 464,812 + Pf6N2E2_396 0.73 +1.1 464,812 + Pf6N2E2_396 0.73 -0.2 464,846 + Pf6N2E2_396 0.82 -1.9 464,857 + Pf6N2E2_396 0.84 -0.6 464,900 + +1.9 464,900 + -0.9 464,901 - +0.4 464,901 - -1.0 464,901 - -1.5 464,913 + +0.1 464,913 + +0.5 464,914 - -1.1 464,914 - +0.8 464,914 - -0.3 464,918 + -0.1 464,918 + +0.9 464,918 + +0.5 464,919 - -1.8 464,919 - +0.7 464,919 - +0.7 464,919 - +2.3 464,919 - +0.5 464,919 - +0.2 464,919 - +2.5 464,930 + -1.5 465,034 + -2.5 465,035 - +1.9 465,084 + -2.6 465,084 + +0.1 465,085 - +0.3 465,085 - -2.7 465,085 - -1.9 465,085 - +0.7 465,085 - +1.1 465,225 + +1.6 465,225 + -0.6 465,226 - -0.3 465,226 - +1.0 465,226 - -0.7 465,226 - -1.3 465,226 - -2.9 465,226 - -0.8 465,226 - -0.2 465,348 + Pf6N2E2_397 0.14 +1.6 465,391 - Pf6N2E2_397 0.16 -1.3 465,404 + Pf6N2E2_397 0.17 -0.1 465,404 + Pf6N2E2_397 0.17 +0.3 465,446 + Pf6N2E2_397 0.19 -0.2 465,446 + Pf6N2E2_397 0.19 +0.4 465,473 + Pf6N2E2_397 0.20 -1.9 465,473 + Pf6N2E2_397 0.20 -0.4 465,473 + Pf6N2E2_397 0.20 +0.2 465,473 + Pf6N2E2_397 0.20 +1.6 465,474 - Pf6N2E2_397 0.20 +1.2 465,474 - Pf6N2E2_397 0.20 -1.9 465,474 - Pf6N2E2_397 0.20 -0.5 465,474 - Pf6N2E2_397 0.20 +2.3 465,474 - Pf6N2E2_397 0.20 -0.8 465,474 - Pf6N2E2_397 0.20 +0.1 465,474 - Pf6N2E2_397 0.20 -0.6 465,551 + Pf6N2E2_397 0.24 -0.5 465,552 - Pf6N2E2_397 0.24 -1.6 465,691 - Pf6N2E2_397 0.31 -0.7 465,820 + Pf6N2E2_397 0.38 +2.4 465,843 + Pf6N2E2_397 0.39 +1.6 465,874 + Pf6N2E2_397 0.41 +1.3 465,875 - Pf6N2E2_397 0.41 -0.3
Or see this region's nucleotide sequence