Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3908 and Pf6N2E2_3909 are separated by 250 nucleotides Pf6N2E2_3909 and Pf6N2E2_3910 are separated by 21 nucleotides Pf6N2E2_3910 and Pf6N2E2_3911 overlap by 4 nucleotides
Pf6N2E2_3908 - Glutamate--cysteine ligase (EC 6.3.2.2), at 4,542,782 to 4,544,380
_3908
Pf6N2E2_3909 - Taurine-binding periplasmic protein TauA, at 4,544,631 to 4,545,608
_3909
Pf6N2E2_3910 - Taurine transport ATP-binding protein TauB, at 4,545,630 to 4,546,424
_3910
Pf6N2E2_3911 - Taurine transport system permease protein TauC, at 4,546,421 to 4,547,260
_3911
Position (kb)
4544
4545
4546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4543.666 kb on + strand, within Pf6N2E2_3908 at 4543.694 kb on + strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.727 kb on + strand, within Pf6N2E2_3908 at 4543.776 kb on - strand, within Pf6N2E2_3908 at 4543.865 kb on + strand, within Pf6N2E2_3908 at 4543.866 kb on - strand, within Pf6N2E2_3908 at 4544.349 kb on - strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.508 kb on - strand at 4544.770 kb on - strand, within Pf6N2E2_3909 at 4545.045 kb on + strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.100 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909 at 4545.570 kb on + strand at 4545.571 kb on - strand at 4545.571 kb on - strand at 4545.580 kb on - strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.667 kb on - strand at 4545.667 kb on - strand at 4546.016 kb on + strand, within Pf6N2E2_3910 at 4546.017 kb on - strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.151 kb on - strand, within Pf6N2E2_3910 at 4546.151 kb on - strand, within Pf6N2E2_3910 at 4546.279 kb on + strand, within Pf6N2E2_3910 at 4546.280 kb on - strand, within Pf6N2E2_3910 at 4546.280 kb on - strand, within Pf6N2E2_3910 at 4546.431 kb on - strand at 4546.485 kb on + strand at 4546.486 kb on - strand at 4546.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 4,543,666 + Pf6N2E2_3908 0.55 -1.5 4,543,694 + Pf6N2E2_3908 0.57 -0.9 4,543,695 - Pf6N2E2_3908 0.57 -1.7 4,543,695 - Pf6N2E2_3908 0.57 -1.8 4,543,697 + Pf6N2E2_3908 0.57 -0.4 4,543,697 + Pf6N2E2_3908 0.57 -0.6 4,543,727 + Pf6N2E2_3908 0.59 -1.4 4,543,776 - Pf6N2E2_3908 0.62 -0.4 4,543,865 + Pf6N2E2_3908 0.68 -0.7 4,543,866 - Pf6N2E2_3908 0.68 -0.4 4,544,349 - +0.6 4,544,374 + +0.5 4,544,374 + +1.2 4,544,374 + -0.6 4,544,375 - +1.7 4,544,375 - +1.5 4,544,375 - +1.7 4,544,508 - -2.5 4,544,770 - Pf6N2E2_3909 0.14 -0.4 4,545,045 + Pf6N2E2_3909 0.42 -2.6 4,545,046 - Pf6N2E2_3909 0.42 +1.2 4,545,046 - Pf6N2E2_3909 0.42 -2.2 4,545,046 - Pf6N2E2_3909 0.42 -1.6 4,545,046 - Pf6N2E2_3909 0.42 +0.2 4,545,100 + Pf6N2E2_3909 0.48 +0.4 4,545,162 + Pf6N2E2_3909 0.54 -0.3 4,545,162 + Pf6N2E2_3909 0.54 -2.9 4,545,162 + Pf6N2E2_3909 0.54 +2.9 4,545,162 + Pf6N2E2_3909 0.54 -2.9 4,545,162 + Pf6N2E2_3909 0.54 -2.3 4,545,163 - Pf6N2E2_3909 0.54 -0.4 4,545,163 - Pf6N2E2_3909 0.54 +1.4 4,545,163 - Pf6N2E2_3909 0.54 -1.0 4,545,163 - Pf6N2E2_3909 0.54 -2.4 4,545,163 - Pf6N2E2_3909 0.54 -0.6 4,545,163 - Pf6N2E2_3909 0.54 -0.4 4,545,336 + Pf6N2E2_3909 0.72 -0.8 4,545,336 + Pf6N2E2_3909 0.72 -0.3 4,545,570 + -0.0 4,545,571 - +0.2 4,545,571 - -0.2 4,545,580 - +0.4 4,545,594 + -1.0 4,545,594 + +0.9 4,545,594 + -2.2 4,545,594 + +0.2 4,545,594 + -1.9 4,545,594 + +0.0 4,545,594 + +0.2 4,545,595 - -1.8 4,545,595 - -0.4 4,545,595 - +0.6 4,545,595 - +1.0 4,545,595 - -0.3 4,545,595 - +1.1 4,545,595 - +0.9 4,545,595 - -0.6 4,545,667 - +0.9 4,545,667 - -1.4 4,546,016 + Pf6N2E2_3910 0.49 +0.2 4,546,017 - Pf6N2E2_3910 0.49 +0.2 4,546,150 + Pf6N2E2_3910 0.65 +2.0 4,546,150 + Pf6N2E2_3910 0.65 -0.7 4,546,150 + Pf6N2E2_3910 0.65 +1.3 4,546,150 + Pf6N2E2_3910 0.65 +0.8 4,546,150 + Pf6N2E2_3910 0.65 +0.2 4,546,151 - Pf6N2E2_3910 0.66 +0.7 4,546,151 - Pf6N2E2_3910 0.66 -0.7 4,546,279 + Pf6N2E2_3910 0.82 -1.6 4,546,280 - Pf6N2E2_3910 0.82 -1.6 4,546,280 - Pf6N2E2_3910 0.82 -2.9 4,546,431 - +0.3 4,546,485 + +0.2 4,546,486 - +0.7 4,546,486 - -1.4
Or see this region's nucleotide sequence