Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotides Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides
Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043
_348
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Position (kb) 415
416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 415.062 kb on + strand at 415.062 kb on + strand at 415.063 kb on - strand at 415.063 kb on - strand at 415.073 kb on + strand at 415.073 kb on + strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.082 kb on + strand at 415.083 kb on - strand at 415.083 kb on - strand at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351 at 417.212 kb on - strand, within Pf6N2E2_351
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 414,849 + Pf6N2E2_348 0.79 -2.4 414,849 + Pf6N2E2_348 0.79 -1.7 414,849 + Pf6N2E2_348 0.79 +0.6 414,849 + Pf6N2E2_348 0.79 -1.8 414,849 + Pf6N2E2_348 0.79 -2.1 414,850 - Pf6N2E2_348 0.79 +3.1 414,850 - Pf6N2E2_348 0.79 +1.5 414,850 - Pf6N2E2_348 0.79 -0.3 414,850 - Pf6N2E2_348 0.79 +1.1 414,927 + Pf6N2E2_348 0.87 -2.3 414,927 + Pf6N2E2_348 0.87 +2.5 415,062 + -0.3 415,062 + +0.9 415,063 - -1.3 415,063 - -1.8 415,073 + -1.4 415,073 + +0.2 415,074 - -0.0 415,074 - +2.3 415,074 - -0.6 415,082 + -0.0 415,083 - +0.4 415,083 - +0.3 415,347 + Pf6N2E2_349 0.42 -2.6 415,515 + Pf6N2E2_349 0.65 -0.1 415,516 - Pf6N2E2_349 0.65 +0.6 415,542 - Pf6N2E2_349 0.68 -2.0 415,660 + Pf6N2E2_349 0.84 +0.7 415,660 + Pf6N2E2_349 0.84 -2.0 415,772 + +0.5 415,773 - -2.4 415,806 + -1.3 415,807 - +1.1 415,807 - -0.3 415,807 - +0.6 415,807 - -2.3 415,865 + -3.1 416,131 + Pf6N2E2_350 0.74 -2.1 416,192 - Pf6N2E2_350 0.90 -0.9 416,231 - +0.5 416,231 - -0.8 416,272 + -0.6 416,272 + +1.1 416,272 + -1.6 416,297 - +1.9 416,323 + -1.2 416,449 + Pf6N2E2_351 0.15 +1.0 416,584 + Pf6N2E2_351 0.24 -1.1 416,584 + Pf6N2E2_351 0.24 -0.0 416,585 - Pf6N2E2_351 0.24 +0.5 417,212 - Pf6N2E2_351 0.67 +1.2
Or see this region's nucleotide sequence