Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3392 and Pf6N2E2_3393 are separated by 657 nucleotides Pf6N2E2_3393 and Pf6N2E2_3394 are separated by 41 nucleotides
Pf6N2E2_3392 - Thermostable 8-oxoguanine DNA glycosylase, at 3,930,618 to 3,931,388
_3392
Pf6N2E2_3393 - Queuosine Biosynthesis QueC ATPase, at 3,932,046 to 3,932,606
_3393
Pf6N2E2_3394 - Ribokinase (EC 2.7.1.15), at 3,932,648 to 3,933,865
_3394
Position (kb)
3932
3933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3931.110 kb on + strand, within Pf6N2E2_3392 at 3931.452 kb on - strand at 3931.477 kb on + strand at 3931.478 kb on - strand at 3931.581 kb on + strand at 3931.581 kb on + strand at 3931.582 kb on - strand at 3931.582 kb on - strand at 3931.613 kb on + strand at 3931.613 kb on + strand at 3931.664 kb on + strand at 3931.664 kb on + strand at 3931.664 kb on + strand at 3931.665 kb on - strand at 3931.697 kb on + strand at 3931.697 kb on + strand at 3931.697 kb on + strand at 3931.697 kb on + strand at 3931.698 kb on - strand at 3931.723 kb on + strand at 3931.723 kb on + strand at 3931.729 kb on - strand at 3931.774 kb on + strand at 3931.814 kb on + strand at 3931.814 kb on + strand at 3931.869 kb on - strand at 3931.880 kb on + strand at 3931.880 kb on + strand at 3931.881 kb on - strand at 3931.881 kb on - strand at 3931.924 kb on + strand at 3931.924 kb on + strand at 3931.934 kb on + strand at 3931.935 kb on - strand at 3932.016 kb on + strand at 3932.016 kb on + strand at 3932.016 kb on + strand at 3932.016 kb on + strand at 3932.016 kb on + strand at 3932.017 kb on - strand at 3932.017 kb on - strand at 3932.017 kb on - strand at 3932.017 kb on - strand at 3932.020 kb on - strand at 3932.036 kb on + strand at 3932.095 kb on + strand at 3932.095 kb on + strand at 3932.095 kb on + strand at 3932.095 kb on + strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.096 kb on - strand at 3932.166 kb on - strand, within Pf6N2E2_3393 at 3932.208 kb on + strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.209 kb on - strand, within Pf6N2E2_3393 at 3932.332 kb on + strand, within Pf6N2E2_3393 at 3932.332 kb on + strand, within Pf6N2E2_3393 at 3932.333 kb on - strand, within Pf6N2E2_3393 at 3932.333 kb on - strand, within Pf6N2E2_3393 at 3932.333 kb on - strand, within Pf6N2E2_3393 at 3932.333 kb on - strand, within Pf6N2E2_3393 at 3932.344 kb on - strand, within Pf6N2E2_3393 at 3932.344 kb on - strand, within Pf6N2E2_3393 at 3932.379 kb on + strand, within Pf6N2E2_3393 at 3932.563 kb on + strand at 3932.564 kb on - strand at 3932.632 kb on - strand at 3932.651 kb on + strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.652 kb on - strand at 3932.679 kb on + strand at 3932.680 kb on - strand at 3932.680 kb on - strand at 3932.680 kb on - strand at 3932.763 kb on + strand at 3932.763 kb on + strand at 3932.763 kb on + strand at 3932.763 kb on + strand at 3932.763 kb on + strand at 3932.764 kb on - strand at 3932.764 kb on - strand at 3932.768 kb on + strand at 3932.768 kb on + strand at 3932.768 kb on + strand at 3932.768 kb on + strand at 3932.768 kb on + strand at 3932.769 kb on - strand at 3932.769 kb on - strand at 3932.769 kb on - strand at 3932.769 kb on - strand at 3932.808 kb on + strand, within Pf6N2E2_3394 at 3932.808 kb on + strand, within Pf6N2E2_3394 at 3932.809 kb on - strand, within Pf6N2E2_3394 at 3932.849 kb on + strand, within Pf6N2E2_3394 at 3932.849 kb on + strand, within Pf6N2E2_3394 at 3932.849 kb on + strand, within Pf6N2E2_3394 at 3932.850 kb on - strand, within Pf6N2E2_3394 at 3932.850 kb on - strand, within Pf6N2E2_3394 at 3932.850 kb on - strand, within Pf6N2E2_3394 at 3932.850 kb on - strand, within Pf6N2E2_3394 at 3932.921 kb on + strand, within Pf6N2E2_3394 at 3932.921 kb on + strand, within Pf6N2E2_3394 at 3932.921 kb on + strand, within Pf6N2E2_3394 at 3932.922 kb on - strand, within Pf6N2E2_3394 at 3932.933 kb on + strand, within Pf6N2E2_3394 at 3932.934 kb on - strand, within Pf6N2E2_3394 at 3933.018 kb on + strand, within Pf6N2E2_3394 at 3933.019 kb on - strand, within Pf6N2E2_3394 at 3933.078 kb on + strand, within Pf6N2E2_3394 at 3933.078 kb on + strand, within Pf6N2E2_3394 at 3933.079 kb on - strand, within Pf6N2E2_3394 at 3933.079 kb on - strand, within Pf6N2E2_3394 at 3933.079 kb on - strand, within Pf6N2E2_3394 at 3933.079 kb on - strand, within Pf6N2E2_3394 at 3933.079 kb on - strand, within Pf6N2E2_3394 at 3933.123 kb on + strand, within Pf6N2E2_3394 at 3933.124 kb on - strand, within Pf6N2E2_3394 at 3933.126 kb on + strand, within Pf6N2E2_3394 at 3933.126 kb on + strand, within Pf6N2E2_3394 at 3933.126 kb on + strand, within Pf6N2E2_3394 at 3933.127 kb on - strand, within Pf6N2E2_3394 at 3933.169 kb on + strand, within Pf6N2E2_3394 at 3933.169 kb on + strand, within Pf6N2E2_3394 at 3933.169 kb on + strand, within Pf6N2E2_3394 at 3933.170 kb on - strand, within Pf6N2E2_3394 at 3933.170 kb on - strand, within Pf6N2E2_3394 at 3933.493 kb on - strand, within Pf6N2E2_3394 at 3933.493 kb on - strand, within Pf6N2E2_3394 at 3933.512 kb on + strand, within Pf6N2E2_3394 at 3933.512 kb on + strand, within Pf6N2E2_3394 at 3933.512 kb on + strand, within Pf6N2E2_3394 at 3933.513 kb on - strand, within Pf6N2E2_3394 at 3933.513 kb on - strand, within Pf6N2E2_3394 at 3933.513 kb on - strand, within Pf6N2E2_3394
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 3,931,110 + Pf6N2E2_3392 0.64 -2.3 3,931,452 - +1.4 3,931,477 + +1.0 3,931,478 - -2.3 3,931,581 + -1.1 3,931,581 + -2.0 3,931,582 - -1.1 3,931,582 - -1.0 3,931,613 + +1.7 3,931,613 + +1.2 3,931,664 + +0.2 3,931,664 + +0.1 3,931,664 + +0.8 3,931,665 - -1.2 3,931,697 + -1.3 3,931,697 + -2.3 3,931,697 + -2.3 3,931,697 + +0.7 3,931,698 - +0.1 3,931,723 + +1.0 3,931,723 + -1.7 3,931,729 - -0.3 3,931,774 + +0.3 3,931,814 + -2.6 3,931,814 + -0.0 3,931,869 - -1.4 3,931,880 + +0.3 3,931,880 + -0.1 3,931,881 - +0.7 3,931,881 - +2.3 3,931,924 + +0.5 3,931,924 + -3.1 3,931,934 + +0.2 3,931,935 - -0.9 3,932,016 + +0.4 3,932,016 + -0.8 3,932,016 + -2.2 3,932,016 + -0.2 3,932,016 + -2.5 3,932,017 - +2.0 3,932,017 - +0.9 3,932,017 - +1.1 3,932,017 - +0.6 3,932,020 - +0.8 3,932,036 + -1.7 3,932,095 + -0.5 3,932,095 + -0.5 3,932,095 + +0.6 3,932,095 + +0.1 3,932,096 - -1.1 3,932,096 - +0.6 3,932,096 - +0.3 3,932,096 - -2.3 3,932,096 - -1.3 3,932,096 - +0.2 3,932,096 - +1.6 3,932,166 - Pf6N2E2_3393 0.21 -1.9 3,932,208 + Pf6N2E2_3393 0.29 +0.3 3,932,209 - Pf6N2E2_3393 0.29 +0.4 3,932,209 - Pf6N2E2_3393 0.29 -1.1 3,932,209 - Pf6N2E2_3393 0.29 +0.5 3,932,209 - Pf6N2E2_3393 0.29 -0.4 3,932,209 - Pf6N2E2_3393 0.29 -2.9 3,932,209 - Pf6N2E2_3393 0.29 -0.8 3,932,332 + Pf6N2E2_3393 0.51 -0.0 3,932,332 + Pf6N2E2_3393 0.51 -1.6 3,932,333 - Pf6N2E2_3393 0.51 -1.3 3,932,333 - Pf6N2E2_3393 0.51 -1.3 3,932,333 - Pf6N2E2_3393 0.51 -1.5 3,932,333 - Pf6N2E2_3393 0.51 +1.0 3,932,344 - Pf6N2E2_3393 0.53 -1.9 3,932,344 - Pf6N2E2_3393 0.53 +1.2 3,932,379 + Pf6N2E2_3393 0.59 -2.7 3,932,563 + +2.4 3,932,564 - +0.4 3,932,632 - +0.8 3,932,651 + -0.6 3,932,652 - +1.0 3,932,652 - +1.5 3,932,652 - -1.0 3,932,652 - -1.8 3,932,652 - -2.5 3,932,652 - +0.3 3,932,652 - -3.4 3,932,679 + +0.2 3,932,680 - +2.9 3,932,680 - -0.1 3,932,680 - +0.3 3,932,763 + +0.4 3,932,763 + +2.2 3,932,763 + -2.5 3,932,763 + +0.2 3,932,763 + +0.8 3,932,764 - +0.2 3,932,764 - +0.5 3,932,768 + +0.3 3,932,768 + -0.1 3,932,768 + +0.3 3,932,768 + +0.2 3,932,768 + -1.1 3,932,769 - -2.8 3,932,769 - -0.6 3,932,769 - +1.8 3,932,769 - -0.0 3,932,808 + Pf6N2E2_3394 0.13 +1.2 3,932,808 + Pf6N2E2_3394 0.13 -0.1 3,932,809 - Pf6N2E2_3394 0.13 +2.5 3,932,849 + Pf6N2E2_3394 0.17 +0.1 3,932,849 + Pf6N2E2_3394 0.17 +1.0 3,932,849 + Pf6N2E2_3394 0.17 -2.8 3,932,850 - Pf6N2E2_3394 0.17 -2.1 3,932,850 - Pf6N2E2_3394 0.17 -1.5 3,932,850 - Pf6N2E2_3394 0.17 +0.3 3,932,850 - Pf6N2E2_3394 0.17 +0.8 3,932,921 + Pf6N2E2_3394 0.22 +1.0 3,932,921 + Pf6N2E2_3394 0.22 -3.3 3,932,921 + Pf6N2E2_3394 0.22 +1.0 3,932,922 - Pf6N2E2_3394 0.22 +0.9 3,932,933 + Pf6N2E2_3394 0.23 +1.0 3,932,934 - Pf6N2E2_3394 0.23 +1.3 3,933,018 + Pf6N2E2_3394 0.30 +0.9 3,933,019 - Pf6N2E2_3394 0.30 +1.7 3,933,078 + Pf6N2E2_3394 0.35 -2.3 3,933,078 + Pf6N2E2_3394 0.35 +1.1 3,933,079 - Pf6N2E2_3394 0.35 -2.3 3,933,079 - Pf6N2E2_3394 0.35 -2.9 3,933,079 - Pf6N2E2_3394 0.35 +0.4 3,933,079 - Pf6N2E2_3394 0.35 -0.8 3,933,079 - Pf6N2E2_3394 0.35 -1.7 3,933,123 + Pf6N2E2_3394 0.39 +0.2 3,933,124 - Pf6N2E2_3394 0.39 +0.8 3,933,126 + Pf6N2E2_3394 0.39 +1.5 3,933,126 + Pf6N2E2_3394 0.39 -2.5 3,933,126 + Pf6N2E2_3394 0.39 -1.1 3,933,127 - Pf6N2E2_3394 0.39 -1.0 3,933,169 + Pf6N2E2_3394 0.43 +0.2 3,933,169 + Pf6N2E2_3394 0.43 +1.8 3,933,169 + Pf6N2E2_3394 0.43 +0.1 3,933,170 - Pf6N2E2_3394 0.43 -1.5 3,933,170 - Pf6N2E2_3394 0.43 +1.0 3,933,493 - Pf6N2E2_3394 0.69 -1.1 3,933,493 - Pf6N2E2_3394 0.69 -3.2 3,933,512 + Pf6N2E2_3394 0.71 +0.9 3,933,512 + Pf6N2E2_3394 0.71 -0.6 3,933,512 + Pf6N2E2_3394 0.71 +1.8 3,933,513 - Pf6N2E2_3394 0.71 +2.0 3,933,513 - Pf6N2E2_3394 0.71 +1.1 3,933,513 - Pf6N2E2_3394 0.71 -0.5
Or see this region's nucleotide sequence