Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2902 and Pf6N2E2_2903 are separated by 140 nucleotides Pf6N2E2_2903 and Pf6N2E2_2904 are separated by 44 nucleotides Pf6N2E2_2904 and Pf6N2E2_2905 are separated by 34 nucleotides
Pf6N2E2_2902 - TonB-dependent hemin , ferrichrome receptor, at 3,391,955 to 3,394,477
_2902
Pf6N2E2_2903 - Heme oxygenase HemO, associated with heme uptake, at 3,394,618 to 3,395,208
_2903
Pf6N2E2_2904 - Hypothetical protein DUF454, at 3,395,253 to 3,395,693
_2904
Pf6N2E2_2905 - CoA transferase, CAIB/BAIF family, at 3,395,728 to 3,397,074
_2905
Position (kb)
3395
3396 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3394.289 kb on + strand at 3394.289 kb on + strand at 3394.290 kb on - strand at 3394.290 kb on - strand at 3394.328 kb on + strand at 3394.328 kb on + strand at 3394.329 kb on - strand at 3394.329 kb on - strand at 3394.329 kb on - strand at 3394.349 kb on + strand at 3394.349 kb on + strand at 3394.350 kb on - strand at 3394.350 kb on - strand at 3394.350 kb on - strand at 3394.445 kb on - strand at 3394.472 kb on - strand at 3394.623 kb on + strand at 3394.624 kb on - strand at 3394.624 kb on - strand at 3394.789 kb on + strand, within Pf6N2E2_2903 at 3394.789 kb on + strand, within Pf6N2E2_2903 at 3394.790 kb on - strand, within Pf6N2E2_2903 at 3394.790 kb on - strand, within Pf6N2E2_2903 at 3394.790 kb on - strand, within Pf6N2E2_2903 at 3394.790 kb on - strand, within Pf6N2E2_2903 at 3395.166 kb on + strand at 3395.167 kb on - strand at 3395.189 kb on - strand at 3395.189 kb on - strand at 3395.301 kb on + strand, within Pf6N2E2_2904 at 3395.566 kb on - strand, within Pf6N2E2_2904 at 3395.566 kb on - strand, within Pf6N2E2_2904 at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.696 kb on + strand at 3395.697 kb on - strand at 3395.697 kb on - strand at 3395.697 kb on - strand at 3395.697 kb on - strand at 3395.697 kb on - strand at 3395.712 kb on + strand at 3395.712 kb on + strand at 3396.010 kb on + strand, within Pf6N2E2_2905 at 3396.010 kb on + strand, within Pf6N2E2_2905 at 3396.011 kb on - strand, within Pf6N2E2_2905 at 3396.551 kb on - strand, within Pf6N2E2_2905
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 3,394,289 + -1.9 3,394,289 + +0.1 3,394,290 - -2.0 3,394,290 - -1.1 3,394,328 + -0.9 3,394,328 + -1.9 3,394,329 - -0.0 3,394,329 - -0.5 3,394,329 - -0.5 3,394,349 + +1.5 3,394,349 + +0.4 3,394,350 - +1.6 3,394,350 - +3.9 3,394,350 - +0.7 3,394,445 - +0.8 3,394,472 - -1.0 3,394,623 + +0.7 3,394,624 - -1.6 3,394,624 - -2.6 3,394,789 + Pf6N2E2_2903 0.29 +1.4 3,394,789 + Pf6N2E2_2903 0.29 -1.8 3,394,790 - Pf6N2E2_2903 0.29 +0.4 3,394,790 - Pf6N2E2_2903 0.29 -1.5 3,394,790 - Pf6N2E2_2903 0.29 -1.6 3,394,790 - Pf6N2E2_2903 0.29 +0.4 3,395,166 + -0.1 3,395,167 - -0.1 3,395,189 - +0.5 3,395,189 - -1.3 3,395,301 + Pf6N2E2_2904 0.11 -1.0 3,395,566 - Pf6N2E2_2904 0.71 -2.8 3,395,566 - Pf6N2E2_2904 0.71 -1.2 3,395,696 + +1.7 3,395,696 + -0.5 3,395,696 + -0.3 3,395,696 + -2.1 3,395,696 + +0.7 3,395,696 + -2.4 3,395,696 + -0.2 3,395,696 + +2.2 3,395,696 + +0.0 3,395,697 - -0.6 3,395,697 - +0.4 3,395,697 - -1.2 3,395,697 - -2.5 3,395,697 - -2.4 3,395,712 + -0.5 3,395,712 + +1.8 3,396,010 + Pf6N2E2_2905 0.21 +0.6 3,396,010 + Pf6N2E2_2905 0.21 -0.0 3,396,011 - Pf6N2E2_2905 0.21 +1.8 3,396,551 - Pf6N2E2_2905 0.61 -2.1
Or see this region's nucleotide sequence