Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2699 and Pf6N2E2_2700 are separated by 431 nucleotides Pf6N2E2_2700 and Pf6N2E2_2701 are separated by 147 nucleotides
Pf6N2E2_2699 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 3,192,596 to 3,193,534
_2699
Pf6N2E2_2700 - Cysteine synthase (EC 2.5.1.47), at 3,193,966 to 3,194,940
_2700
Pf6N2E2_2701 - FIG00954689: hypothetical protein, at 3,195,088 to 3,196,155
_2701
Position (kb)
3193
3194
3195 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3193.189 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.261 kb on - strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.353 kb on + strand, within Pf6N2E2_2699 at 3193.383 kb on + strand, within Pf6N2E2_2699 at 3193.384 kb on - strand, within Pf6N2E2_2699 at 3193.396 kb on + strand, within Pf6N2E2_2699 at 3193.397 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.399 kb on - strand, within Pf6N2E2_2699 at 3193.473 kb on + strand at 3193.473 kb on + strand at 3193.575 kb on - strand at 3193.586 kb on + strand at 3193.675 kb on + strand at 3193.675 kb on + strand at 3193.725 kb on + strand at 3193.725 kb on + strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.726 kb on - strand at 3193.889 kb on + strand at 3193.889 kb on + strand at 3193.890 kb on - strand at 3193.890 kb on - strand at 3194.021 kb on + strand at 3194.021 kb on + strand at 3194.022 kb on - strand at 3194.189 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.373 kb on - strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.522 kb on + strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700 at 3194.523 kb on - strand, within Pf6N2E2_2700 at 3194.678 kb on + strand, within Pf6N2E2_2700 at 3194.678 kb on + strand, within Pf6N2E2_2700 at 3194.679 kb on - strand, within Pf6N2E2_2700 at 3194.679 kb on - strand, within Pf6N2E2_2700 at 3194.898 kb on + strand at 3194.898 kb on + strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.899 kb on - strand at 3194.929 kb on + strand at 3194.930 kb on - strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.931 kb on + strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.932 kb on - strand at 3194.968 kb on + strand at 3194.969 kb on - strand at 3194.982 kb on + strand at 3194.982 kb on + strand at 3194.983 kb on - strand at 3195.048 kb on + strand at 3195.048 kb on + strand at 3195.048 kb on + strand at 3195.049 kb on - strand at 3195.049 kb on - strand at 3195.049 kb on - strand at 3195.169 kb on + strand at 3195.170 kb on - strand at 3195.370 kb on + strand, within Pf6N2E2_2701 at 3195.610 kb on + strand, within Pf6N2E2_2701 at 3195.611 kb on - strand, within Pf6N2E2_2701 at 3195.611 kb on - strand, within Pf6N2E2_2701 at 3195.802 kb on + strand, within Pf6N2E2_2701 at 3195.880 kb on + strand, within Pf6N2E2_2701 at 3195.880 kb on + strand, within Pf6N2E2_2701 at 3195.881 kb on - strand, within Pf6N2E2_2701
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 3,193,189 - Pf6N2E2_2699 0.63 +2.4 3,193,261 - Pf6N2E2_2699 0.71 +2.2 3,193,261 - Pf6N2E2_2699 0.71 +2.4 3,193,261 - Pf6N2E2_2699 0.71 +1.3 3,193,353 + Pf6N2E2_2699 0.81 +2.6 3,193,353 + Pf6N2E2_2699 0.81 +2.8 3,193,353 + Pf6N2E2_2699 0.81 +1.8 3,193,383 + Pf6N2E2_2699 0.84 +3.8 3,193,384 - Pf6N2E2_2699 0.84 +2.7 3,193,396 + Pf6N2E2_2699 0.85 +2.4 3,193,397 - Pf6N2E2_2699 0.85 +0.9 3,193,399 - Pf6N2E2_2699 0.86 +1.7 3,193,399 - Pf6N2E2_2699 0.86 +1.4 3,193,399 - Pf6N2E2_2699 0.86 +1.1 3,193,473 + +1.7 3,193,473 + +4.1 3,193,575 - -0.2 3,193,586 + -1.5 3,193,675 + -2.3 3,193,675 + +1.3 3,193,725 + +0.0 3,193,725 + +0.5 3,193,726 - +1.8 3,193,726 - +0.4 3,193,726 - +0.3 3,193,726 - -1.9 3,193,889 + -2.9 3,193,889 + +0.7 3,193,890 - +1.7 3,193,890 - -0.4 3,194,021 + -2.2 3,194,021 + -0.1 3,194,022 - -1.8 3,194,189 - Pf6N2E2_2700 0.23 -3.8 3,194,373 - Pf6N2E2_2700 0.42 -2.7 3,194,373 - Pf6N2E2_2700 0.42 +0.5 3,194,373 - Pf6N2E2_2700 0.42 +0.4 3,194,373 - Pf6N2E2_2700 0.42 +0.4 3,194,522 + Pf6N2E2_2700 0.57 -1.2 3,194,522 + Pf6N2E2_2700 0.57 -1.5 3,194,522 + Pf6N2E2_2700 0.57 +0.2 3,194,522 + Pf6N2E2_2700 0.57 -3.3 3,194,522 + Pf6N2E2_2700 0.57 -2.8 3,194,523 - Pf6N2E2_2700 0.57 +1.0 3,194,523 - Pf6N2E2_2700 0.57 -1.7 3,194,678 + Pf6N2E2_2700 0.73 -1.5 3,194,678 + Pf6N2E2_2700 0.73 -2.2 3,194,679 - Pf6N2E2_2700 0.73 -3.0 3,194,679 - Pf6N2E2_2700 0.73 +2.1 3,194,898 + +1.0 3,194,898 + +0.4 3,194,899 - +0.7 3,194,899 - +2.8 3,194,899 - -2.1 3,194,899 - +2.4 3,194,929 + -0.8 3,194,930 - +2.0 3,194,931 + +0.3 3,194,931 + -3.4 3,194,931 + -2.4 3,194,931 + +1.1 3,194,931 + -3.4 3,194,932 - -0.2 3,194,932 - +0.3 3,194,932 - +0.4 3,194,932 - +0.8 3,194,932 - +0.6 3,194,932 - -2.4 3,194,932 - +1.0 3,194,968 + -0.8 3,194,969 - -2.7 3,194,982 + -0.4 3,194,982 + +0.8 3,194,983 - -0.6 3,195,048 + +0.2 3,195,048 + -0.6 3,195,048 + -0.5 3,195,049 - +0.2 3,195,049 - +2.1 3,195,049 - -3.0 3,195,169 + +0.2 3,195,170 - -2.8 3,195,370 + Pf6N2E2_2701 0.26 +0.2 3,195,610 + Pf6N2E2_2701 0.49 -1.6 3,195,611 - Pf6N2E2_2701 0.49 -1.6 3,195,611 - Pf6N2E2_2701 0.49 -0.6 3,195,802 + Pf6N2E2_2701 0.67 +0.5 3,195,880 + Pf6N2E2_2701 0.74 -0.6 3,195,880 + Pf6N2E2_2701 0.74 -0.0 3,195,881 - Pf6N2E2_2701 0.74 -0.4
Or see this region's nucleotide sequence