Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2500 and Pf6N2E2_2501 are separated by 155 nucleotides Pf6N2E2_2501 and Pf6N2E2_2502 are separated by 124 nucleotides Pf6N2E2_2502 and Pf6N2E2_2503 are separated by 235 nucleotides
Pf6N2E2_2500 - Putative membrane protein, at 2,949,145 to 2,950,236
_2500
Pf6N2E2_2501 - hypothetical protein, at 2,950,392 to 2,950,514
_2501
Pf6N2E2_2502 - O-antigen acetylase, at 2,950,639 to 2,952,537
_2502
Pf6N2E2_2503 - Glycosyl transferase, group 1 family protein, at 2,952,773 to 2,954,122
_2503
Position (kb)
2950
2951
2952
2953 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2950.076 kb on - strand, within Pf6N2E2_2500 at 2950.118 kb on + strand, within Pf6N2E2_2500 at 2950.192 kb on + strand at 2950.211 kb on - strand at 2950.259 kb on + strand at 2950.282 kb on + strand at 2950.310 kb on + strand at 2950.379 kb on - strand at 2950.457 kb on - strand, within Pf6N2E2_2501 at 2950.457 kb on - strand, within Pf6N2E2_2501 at 2950.459 kb on - strand, within Pf6N2E2_2501 at 2950.540 kb on - strand at 2950.618 kb on - strand at 2950.785 kb on - strand at 2951.169 kb on + strand, within Pf6N2E2_2502 at 2951.596 kb on + strand, within Pf6N2E2_2502 at 2951.613 kb on - strand, within Pf6N2E2_2502 at 2951.647 kb on - strand, within Pf6N2E2_2502 at 2951.720 kb on + strand, within Pf6N2E2_2502 at 2951.744 kb on + strand, within Pf6N2E2_2502 at 2951.745 kb on - strand, within Pf6N2E2_2502 at 2952.013 kb on + strand, within Pf6N2E2_2502 at 2952.218 kb on + strand, within Pf6N2E2_2502 at 2952.330 kb on + strand, within Pf6N2E2_2502 at 2952.332 kb on + strand, within Pf6N2E2_2502 at 2952.400 kb on + strand at 2952.492 kb on + strand at 2952.492 kb on + strand at 2952.557 kb on + strand at 2952.577 kb on + strand at 2952.577 kb on + strand at 2952.577 kb on + strand at 2952.722 kb on + strand at 2952.750 kb on + strand at 2952.751 kb on - strand at 2952.813 kb on + strand at 2952.814 kb on - strand at 2952.814 kb on - strand at 2952.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,950,076 - Pf6N2E2_2500 0.85 -1.0 2,950,118 + Pf6N2E2_2500 0.89 -2.0 2,950,192 + -2.2 2,950,211 - +0.7 2,950,259 + -0.4 2,950,282 + -0.4 2,950,310 + -2.2 2,950,379 - +1.5 2,950,457 - Pf6N2E2_2501 0.53 -2.8 2,950,457 - Pf6N2E2_2501 0.53 -1.5 2,950,459 - Pf6N2E2_2501 0.54 +0.7 2,950,540 - -2.1 2,950,618 - +0.3 2,950,785 - +1.1 2,951,169 + Pf6N2E2_2502 0.28 -1.7 2,951,596 + Pf6N2E2_2502 0.50 -0.2 2,951,613 - Pf6N2E2_2502 0.51 -0.2 2,951,647 - Pf6N2E2_2502 0.53 -2.7 2,951,720 + Pf6N2E2_2502 0.57 -1.3 2,951,744 + Pf6N2E2_2502 0.58 +0.2 2,951,745 - Pf6N2E2_2502 0.58 -1.7 2,952,013 + Pf6N2E2_2502 0.72 +0.2 2,952,218 + Pf6N2E2_2502 0.83 -3.3 2,952,330 + Pf6N2E2_2502 0.89 +0.0 2,952,332 + Pf6N2E2_2502 0.89 -0.2 2,952,400 + +1.2 2,952,492 + +0.8 2,952,492 + +0.3 2,952,557 + +0.3 2,952,577 + -0.1 2,952,577 + +1.3 2,952,577 + -0.0 2,952,722 + -0.4 2,952,750 + +1.6 2,952,751 - -1.3 2,952,813 + +0.5 2,952,814 - +0.3 2,952,814 - +1.3 2,952,814 - -1.4
Or see this region's nucleotide sequence