Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2470 and Pf6N2E2_2471 are separated by 164 nucleotides Pf6N2E2_2471 and Pf6N2E2_2472 are separated by 202 nucleotides Pf6N2E2_2472 and Pf6N2E2_2473 are separated by 319 nucleotides
Pf6N2E2_2470 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 2,918,306 to 2,919,382
_2470
Pf6N2E2_2471 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,919,547 to 2,919,828
_2471
Pf6N2E2_2472 - hypothetical protein, at 2,920,031 to 2,920,144
_2472
Pf6N2E2_2473 - Major outer membrane lipoprotein I, at 2,920,464 to 2,920,715
_2473
Position (kb)
2919
2920 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.570 kb on + strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.571 kb on - strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.588 kb on + strand, within Pf6N2E2_2470 at 2918.589 kb on - strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.642 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.762 kb on + strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.763 kb on - strand, within Pf6N2E2_2470 at 2918.990 kb on + strand, within Pf6N2E2_2470 at 2918.991 kb on - strand, within Pf6N2E2_2470 at 2919.032 kb on + strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.033 kb on - strand, within Pf6N2E2_2470 at 2919.272 kb on + strand, within Pf6N2E2_2470 at 2919.273 kb on - strand, within Pf6N2E2_2470 at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.417 kb on + strand at 2919.418 kb on - strand at 2919.418 kb on - strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.451 kb on + strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.452 kb on - strand at 2919.478 kb on + strand at 2919.495 kb on - strand at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.584 kb on + strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.585 kb on - strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.596 kb on + strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.597 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.639 kb on - strand, within Pf6N2E2_2471 at 2919.710 kb on + strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.711 kb on - strand, within Pf6N2E2_2471 at 2919.715 kb on - strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.743 kb on + strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.744 kb on - strand, within Pf6N2E2_2471 at 2919.753 kb on - strand, within Pf6N2E2_2471 at 2919.832 kb on + strand at 2919.833 kb on - strand at 2919.861 kb on + strand at 2919.861 kb on + strand at 2919.867 kb on - strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.895 kb on + strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.896 kb on - strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.965 kb on + strand at 2919.966 kb on - strand at 2919.966 kb on - strand at 2919.966 kb on - strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.968 kb on + strand at 2919.969 kb on - strand at 2920.059 kb on + strand, within Pf6N2E2_2472 at 2920.060 kb on - strand, within Pf6N2E2_2472 at 2920.077 kb on + strand, within Pf6N2E2_2472 at 2920.078 kb on - strand, within Pf6N2E2_2472 at 2920.121 kb on - strand, within Pf6N2E2_2472 at 2920.124 kb on + strand, within Pf6N2E2_2472 at 2920.125 kb on - strand, within Pf6N2E2_2472 at 2920.139 kb on + strand at 2920.139 kb on + strand at 2920.140 kb on - strand at 2920.224 kb on + strand at 2920.224 kb on + strand at 2920.225 kb on - strand at 2920.373 kb on - strand at 2920.376 kb on + strand at 2920.376 kb on + strand at 2920.377 kb on - strand at 2920.379 kb on + strand at 2920.379 kb on + strand at 2920.379 kb on + strand at 2920.380 kb on - strand at 2920.380 kb on - strand at 2920.385 kb on + strand at 2920.385 kb on + strand at 2920.392 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.395 kb on + strand at 2920.399 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.439 kb on + strand at 2920.440 kb on - strand at 2920.440 kb on - strand at 2920.608 kb on + strand, within Pf6N2E2_2473 at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.713 kb on + strand at 2920.714 kb on - strand at 2920.716 kb on + strand at 2920.717 kb on - strand at 2920.806 kb on + strand at 2920.806 kb on + strand at 2920.807 kb on - strand at 2920.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,918,570 + Pf6N2E2_2470 0.25 -0.2 2,918,570 + Pf6N2E2_2470 0.25 +1.1 2,918,570 + Pf6N2E2_2470 0.25 -2.9 2,918,570 + Pf6N2E2_2470 0.25 -0.2 2,918,571 - Pf6N2E2_2470 0.25 -1.4 2,918,571 - Pf6N2E2_2470 0.25 -3.6 2,918,588 + Pf6N2E2_2470 0.26 -2.6 2,918,588 + Pf6N2E2_2470 0.26 +0.8 2,918,588 + Pf6N2E2_2470 0.26 +0.3 2,918,589 - Pf6N2E2_2470 0.26 -0.6 2,918,642 + Pf6N2E2_2470 0.31 +0.4 2,918,642 + Pf6N2E2_2470 0.31 +0.8 2,918,762 + Pf6N2E2_2470 0.42 -1.0 2,918,762 + Pf6N2E2_2470 0.42 -0.3 2,918,762 + Pf6N2E2_2470 0.42 -0.7 2,918,762 + Pf6N2E2_2470 0.42 +2.2 2,918,763 - Pf6N2E2_2470 0.42 -1.8 2,918,763 - Pf6N2E2_2470 0.42 +0.2 2,918,763 - Pf6N2E2_2470 0.42 -1.8 2,918,763 - Pf6N2E2_2470 0.42 +1.5 2,918,990 + Pf6N2E2_2470 0.64 -3.2 2,918,991 - Pf6N2E2_2470 0.64 -1.5 2,919,032 + Pf6N2E2_2470 0.67 -1.4 2,919,033 - Pf6N2E2_2470 0.68 -0.9 2,919,033 - Pf6N2E2_2470 0.68 -2.2 2,919,272 + Pf6N2E2_2470 0.90 -0.9 2,919,273 - Pf6N2E2_2470 0.90 -3.1 2,919,417 + -0.7 2,919,417 + -1.5 2,919,417 + -0.1 2,919,417 + -0.1 2,919,418 - -2.8 2,919,418 - -0.4 2,919,451 + -2.3 2,919,451 + -0.0 2,919,451 + -0.4 2,919,451 + +1.0 2,919,452 - -1.5 2,919,452 - -1.5 2,919,452 - -2.9 2,919,452 - -1.1 2,919,478 + +0.6 2,919,495 - +0.4 2,919,584 + Pf6N2E2_2471 0.13 +2.5 2,919,584 + Pf6N2E2_2471 0.13 -0.5 2,919,584 + Pf6N2E2_2471 0.13 -1.2 2,919,585 - Pf6N2E2_2471 0.13 -2.5 2,919,585 - Pf6N2E2_2471 0.13 -0.5 2,919,585 - Pf6N2E2_2471 0.13 -1.0 2,919,585 - Pf6N2E2_2471 0.13 -2.7 2,919,596 + Pf6N2E2_2471 0.17 -0.3 2,919,596 + Pf6N2E2_2471 0.17 -2.3 2,919,596 + Pf6N2E2_2471 0.17 +0.2 2,919,596 + Pf6N2E2_2471 0.17 -1.4 2,919,597 - Pf6N2E2_2471 0.18 -2.1 2,919,597 - Pf6N2E2_2471 0.18 +1.2 2,919,597 - Pf6N2E2_2471 0.18 -2.6 2,919,639 - Pf6N2E2_2471 0.33 -0.7 2,919,639 - Pf6N2E2_2471 0.33 +1.2 2,919,639 - Pf6N2E2_2471 0.33 -3.1 2,919,710 + Pf6N2E2_2471 0.58 -2.3 2,919,711 - Pf6N2E2_2471 0.58 -0.6 2,919,711 - Pf6N2E2_2471 0.58 -1.3 2,919,711 - Pf6N2E2_2471 0.58 -2.0 2,919,715 - Pf6N2E2_2471 0.60 -3.4 2,919,743 + Pf6N2E2_2471 0.70 -1.2 2,919,743 + Pf6N2E2_2471 0.70 +0.6 2,919,743 + Pf6N2E2_2471 0.70 -1.2 2,919,744 - Pf6N2E2_2471 0.70 -1.8 2,919,744 - Pf6N2E2_2471 0.70 -1.1 2,919,744 - Pf6N2E2_2471 0.70 -1.8 2,919,744 - Pf6N2E2_2471 0.70 -2.5 2,919,744 - Pf6N2E2_2471 0.70 -0.2 2,919,744 - Pf6N2E2_2471 0.70 -2.0 2,919,744 - Pf6N2E2_2471 0.70 -3.1 2,919,744 - Pf6N2E2_2471 0.70 -0.0 2,919,744 - Pf6N2E2_2471 0.70 -0.8 2,919,744 - Pf6N2E2_2471 0.70 -3.6 2,919,753 - Pf6N2E2_2471 0.73 -1.5 2,919,832 + -2.4 2,919,833 - -2.9 2,919,861 + -1.8 2,919,861 + -1.7 2,919,867 - -3.2 2,919,895 + -1.8 2,919,895 + +1.1 2,919,895 + -2.6 2,919,895 + -3.2 2,919,896 - -1.9 2,919,896 - -2.3 2,919,896 - -1.5 2,919,896 - -4.0 2,919,896 - -0.5 2,919,896 - -3.2 2,919,896 - -3.2 2,919,896 - -0.9 2,919,896 - -1.0 2,919,965 + +2.2 2,919,965 + +0.3 2,919,965 + -2.6 2,919,965 + -3.7 2,919,966 - -3.8 2,919,966 - -3.3 2,919,966 - -1.3 2,919,968 + +0.4 2,919,968 + -0.3 2,919,968 + -0.8 2,919,968 + +1.2 2,919,969 - -2.2 2,920,059 + Pf6N2E2_2472 0.25 +0.7 2,920,060 - Pf6N2E2_2472 0.25 -3.5 2,920,077 + Pf6N2E2_2472 0.40 -1.5 2,920,078 - Pf6N2E2_2472 0.41 -3.5 2,920,121 - Pf6N2E2_2472 0.79 -1.4 2,920,124 + Pf6N2E2_2472 0.82 -0.8 2,920,125 - Pf6N2E2_2472 0.82 -3.1 2,920,139 + +1.4 2,920,139 + +0.5 2,920,140 - -1.4 2,920,224 + -1.0 2,920,224 + -0.0 2,920,225 - -3.7 2,920,373 - -2.3 2,920,376 + -3.7 2,920,376 + -2.4 2,920,377 - -2.7 2,920,379 + -3.9 2,920,379 + -2.9 2,920,379 + -3.1 2,920,380 - -2.4 2,920,380 - -1.4 2,920,385 + +1.5 2,920,385 + -0.2 2,920,392 + -1.8 2,920,395 + -2.7 2,920,395 + -2.6 2,920,395 + -1.7 2,920,395 + -2.4 2,920,395 + -1.1 2,920,395 + -2.2 2,920,395 + -2.6 2,920,399 + -3.5 2,920,439 + -2.1 2,920,439 + -2.7 2,920,439 + -1.3 2,920,439 + -4.5 2,920,439 + -3.2 2,920,439 + -2.7 2,920,440 - -3.4 2,920,440 - +0.7 2,920,608 + Pf6N2E2_2473 0.57 -3.4 2,920,713 + -3.7 2,920,713 + -2.5 2,920,713 + -2.7 2,920,713 + -3.7 2,920,713 + -4.6 2,920,714 - -3.1 2,920,716 + -3.1 2,920,717 - -2.1 2,920,806 + -3.2 2,920,806 + -1.9 2,920,807 - -1.0 2,920,807 - -1.6
Or see this region's nucleotide sequence