Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2417 and Pf6N2E2_2418 overlap by 4 nucleotides Pf6N2E2_2418 and Pf6N2E2_2419 overlap by 17 nucleotides Pf6N2E2_2419 and Pf6N2E2_2420 are separated by 30 nucleotides
Pf6N2E2_2417 - Sensor histidine kinase, at 2,869,669 to 2,870,562
_2417
Pf6N2E2_2418 - DNA-binding response regulator, LuxR family, at 2,870,559 to 2,871,218
_2418
Pf6N2E2_2419 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 2,871,202 to 2,872,119
_2419
Pf6N2E2_2420 - hypothetical protein, at 2,872,150 to 2,872,317
_2420
Position (kb)
2870
2871
2872 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2869.669 kb on - strand at 2869.669 kb on - strand at 2869.672 kb on + strand at 2869.673 kb on - strand at 2869.673 kb on - strand at 2869.673 kb on - strand at 2869.673 kb on - strand at 2869.673 kb on - strand at 2869.673 kb on - strand at 2869.736 kb on + strand at 2869.736 kb on + strand at 2869.736 kb on + strand at 2869.736 kb on + strand at 2869.736 kb on + strand at 2869.737 kb on - strand at 2869.737 kb on - strand at 2869.737 kb on - strand at 2869.739 kb on + strand at 2869.740 kb on - strand at 2869.740 kb on - strand at 2869.740 kb on - strand at 2869.746 kb on + strand at 2869.746 kb on + strand at 2869.746 kb on + strand at 2869.746 kb on + strand at 2869.747 kb on - strand at 2869.777 kb on + strand, within Pf6N2E2_2417 at 2869.778 kb on - strand, within Pf6N2E2_2417 at 2869.789 kb on + strand, within Pf6N2E2_2417 at 2869.815 kb on - strand, within Pf6N2E2_2417 at 2869.815 kb on - strand, within Pf6N2E2_2417 at 2870.315 kb on + strand, within Pf6N2E2_2417 at 2870.315 kb on + strand, within Pf6N2E2_2417 at 2870.316 kb on - strand, within Pf6N2E2_2417 at 2870.541 kb on + strand at 2870.547 kb on + strand at 2870.548 kb on - strand at 2870.548 kb on - strand at 2870.548 kb on - strand at 2870.548 kb on - strand at 2870.814 kb on - strand, within Pf6N2E2_2418 at 2871.044 kb on - strand, within Pf6N2E2_2418 at 2871.082 kb on + strand, within Pf6N2E2_2418 at 2871.082 kb on + strand, within Pf6N2E2_2418 at 2871.082 kb on + strand, within Pf6N2E2_2418 at 2871.305 kb on - strand, within Pf6N2E2_2419 at 2871.305 kb on - strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.599 kb on + strand, within Pf6N2E2_2419 at 2871.600 kb on - strand, within Pf6N2E2_2419 at 2871.644 kb on + strand, within Pf6N2E2_2419 at 2871.644 kb on + strand, within Pf6N2E2_2419 at 2871.862 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2872.195 kb on - strand, within Pf6N2E2_2420 at 2872.195 kb on - strand, within Pf6N2E2_2420
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,869,669 - -0.4 2,869,669 - +0.5 2,869,672 + +2.9 2,869,673 - -2.6 2,869,673 - +0.5 2,869,673 - -2.3 2,869,673 - -1.8 2,869,673 - -1.7 2,869,673 - +0.9 2,869,736 + +1.7 2,869,736 + -1.1 2,869,736 + -0.7 2,869,736 + -1.4 2,869,736 + -1.6 2,869,737 - -0.6 2,869,737 - -1.8 2,869,737 - +0.4 2,869,739 + -2.4 2,869,740 - -2.0 2,869,740 - -0.1 2,869,740 - +0.1 2,869,746 + +0.8 2,869,746 + -0.6 2,869,746 + +0.5 2,869,746 + -1.0 2,869,747 - +0.3 2,869,777 + Pf6N2E2_2417 0.12 +2.3 2,869,778 - Pf6N2E2_2417 0.12 -1.1 2,869,789 + Pf6N2E2_2417 0.13 -0.8 2,869,815 - Pf6N2E2_2417 0.16 +1.2 2,869,815 - Pf6N2E2_2417 0.16 -0.6 2,870,315 + Pf6N2E2_2417 0.72 +0.6 2,870,315 + Pf6N2E2_2417 0.72 +2.3 2,870,316 - Pf6N2E2_2417 0.72 +0.9 2,870,541 + -1.0 2,870,547 + +1.3 2,870,548 - +0.8 2,870,548 - -1.0 2,870,548 - -0.7 2,870,548 - +0.8 2,870,814 - Pf6N2E2_2418 0.39 -0.7 2,871,044 - Pf6N2E2_2418 0.73 -1.6 2,871,082 + Pf6N2E2_2418 0.79 -0.1 2,871,082 + Pf6N2E2_2418 0.79 +1.8 2,871,082 + Pf6N2E2_2418 0.79 -2.2 2,871,305 - Pf6N2E2_2419 0.11 +0.2 2,871,305 - Pf6N2E2_2419 0.11 -2.2 2,871,432 + Pf6N2E2_2419 0.25 -0.4 2,871,432 + Pf6N2E2_2419 0.25 -1.9 2,871,432 + Pf6N2E2_2419 0.25 +1.3 2,871,432 + Pf6N2E2_2419 0.25 -0.2 2,871,432 + Pf6N2E2_2419 0.25 -0.4 2,871,432 + Pf6N2E2_2419 0.25 -2.9 2,871,433 - Pf6N2E2_2419 0.25 +0.4 2,871,433 - Pf6N2E2_2419 0.25 +3.1 2,871,433 - Pf6N2E2_2419 0.25 -1.9 2,871,599 + Pf6N2E2_2419 0.43 -2.0 2,871,600 - Pf6N2E2_2419 0.43 +1.7 2,871,644 + Pf6N2E2_2419 0.48 -0.7 2,871,644 + Pf6N2E2_2419 0.48 -1.0 2,871,862 + Pf6N2E2_2419 0.72 +0.8 2,871,948 + Pf6N2E2_2419 0.81 +0.2 2,871,948 + Pf6N2E2_2419 0.81 -3.0 2,871,949 - Pf6N2E2_2419 0.81 +0.5 2,871,949 - Pf6N2E2_2419 0.81 -0.6 2,871,949 - Pf6N2E2_2419 0.81 +1.4 2,871,949 - Pf6N2E2_2419 0.81 -1.9 2,871,949 - Pf6N2E2_2419 0.81 +1.1 2,872,195 - Pf6N2E2_2420 0.27 -1.2 2,872,195 - Pf6N2E2_2420 0.27 -0.1
Or see this region's nucleotide sequence