Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2399 and Pf6N2E2_2400 overlap by 25 nucleotides Pf6N2E2_2400 and Pf6N2E2_2401 are separated by 152 nucleotides Pf6N2E2_2401 and Pf6N2E2_2402 are separated by 174 nucleotides Pf6N2E2_2402 and Pf6N2E2_2403 are separated by 98 nucleotides
Pf6N2E2_2399 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 2,838,746 to 2,839,615
_2399
Pf6N2E2_2400 - Thiol:disulfide interchange protein DsbG precursor, at 2,839,591 to 2,840,358
_2400
Pf6N2E2_2401 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 2,840,511 to 2,841,323
_2401
Pf6N2E2_2402 - hypothetical protein, at 2,841,498 to 2,841,761
_2402
Pf6N2E2_2403 - Pyoverdine chromophore precursor synthetase PvdL, at 2,841,860 to 2,854,846
_2403
Position (kb)
2840
2841
2842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2839.762 kb on + strand, within Pf6N2E2_2400 at 2839.763 kb on - strand, within Pf6N2E2_2400 at 2839.786 kb on + strand, within Pf6N2E2_2400 at 2839.787 kb on - strand, within Pf6N2E2_2400 at 2839.829 kb on - strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.880 kb on - strand, within Pf6N2E2_2400 at 2839.966 kb on + strand, within Pf6N2E2_2400 at 2839.966 kb on + strand, within Pf6N2E2_2400 at 2840.095 kb on - strand, within Pf6N2E2_2400 at 2840.192 kb on + strand, within Pf6N2E2_2400 at 2840.193 kb on - strand, within Pf6N2E2_2400 at 2840.357 kb on - strand at 2840.491 kb on + strand at 2840.579 kb on + strand at 2840.580 kb on - strand at 2840.758 kb on - strand, within Pf6N2E2_2401 at 2840.818 kb on + strand, within Pf6N2E2_2401 at 2840.818 kb on + strand, within Pf6N2E2_2401 at 2840.819 kb on - strand, within Pf6N2E2_2401 at 2840.929 kb on + strand, within Pf6N2E2_2401 at 2840.930 kb on - strand, within Pf6N2E2_2401 at 2840.933 kb on - strand, within Pf6N2E2_2401 at 2840.965 kb on + strand, within Pf6N2E2_2401 at 2840.965 kb on + strand, within Pf6N2E2_2401 at 2840.965 kb on + strand, within Pf6N2E2_2401 at 2840.965 kb on + strand, within Pf6N2E2_2401 at 2840.965 kb on + strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2840.966 kb on - strand, within Pf6N2E2_2401 at 2841.079 kb on + strand, within Pf6N2E2_2401 at 2841.079 kb on + strand, within Pf6N2E2_2401 at 2841.241 kb on + strand, within Pf6N2E2_2401 at 2841.241 kb on + strand, within Pf6N2E2_2401 at 2841.241 kb on + strand, within Pf6N2E2_2401 at 2841.242 kb on - strand at 2841.242 kb on - strand at 2841.242 kb on - strand at 2841.242 kb on - strand at 2841.286 kb on + strand at 2841.286 kb on + strand at 2841.287 kb on - strand at 2841.287 kb on - strand at 2841.289 kb on + strand at 2841.290 kb on - strand at 2841.290 kb on - strand at 2841.290 kb on - strand at 2841.290 kb on - strand at 2841.290 kb on - strand at 2841.290 kb on - strand at 2841.401 kb on - strand at 2841.401 kb on - strand at 2841.401 kb on - strand at 2841.423 kb on - strand at 2841.423 kb on - strand at 2841.436 kb on - strand at 2841.436 kb on - strand at 2841.436 kb on - strand at 2841.503 kb on + strand at 2841.633 kb on + strand, within Pf6N2E2_2402 at 2841.633 kb on + strand, within Pf6N2E2_2402 at 2841.634 kb on - strand, within Pf6N2E2_2402 at 2841.801 kb on - strand at 2841.847 kb on - strand at 2841.847 kb on - strand at 2841.861 kb on + strand at 2841.861 kb on + strand at 2841.861 kb on + strand at 2841.862 kb on - strand at 2841.945 kb on + strand at 2841.946 kb on - strand at 2841.946 kb on - strand at 2841.995 kb on + strand at 2841.996 kb on - strand at 2842.275 kb on + strand at 2842.275 kb on + strand at 2842.275 kb on + strand at 2842.276 kb on - strand at 2842.276 kb on - strand at 2842.276 kb on - strand at 2842.276 kb on - strand at 2842.276 kb on - strand at 2842.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,839,762 + Pf6N2E2_2400 0.22 -1.1 2,839,763 - Pf6N2E2_2400 0.22 +0.7 2,839,786 + Pf6N2E2_2400 0.25 +0.2 2,839,787 - Pf6N2E2_2400 0.26 -0.1 2,839,829 - Pf6N2E2_2400 0.31 +2.2 2,839,879 + Pf6N2E2_2400 0.38 +1.0 2,839,879 + Pf6N2E2_2400 0.38 -2.6 2,839,879 + Pf6N2E2_2400 0.38 -0.5 2,839,879 + Pf6N2E2_2400 0.38 -2.5 2,839,880 - Pf6N2E2_2400 0.38 +0.1 2,839,966 + Pf6N2E2_2400 0.49 +1.1 2,839,966 + Pf6N2E2_2400 0.49 +0.2 2,840,095 - Pf6N2E2_2400 0.66 -2.3 2,840,192 + Pf6N2E2_2400 0.78 -2.0 2,840,193 - Pf6N2E2_2400 0.78 -0.7 2,840,357 - -1.6 2,840,491 + -0.8 2,840,579 + +0.7 2,840,580 - -1.8 2,840,758 - Pf6N2E2_2401 0.30 +0.9 2,840,818 + Pf6N2E2_2401 0.38 +0.5 2,840,818 + Pf6N2E2_2401 0.38 +0.6 2,840,819 - Pf6N2E2_2401 0.38 +1.8 2,840,929 + Pf6N2E2_2401 0.51 +1.5 2,840,930 - Pf6N2E2_2401 0.52 +0.8 2,840,933 - Pf6N2E2_2401 0.52 +1.6 2,840,965 + Pf6N2E2_2401 0.56 +0.5 2,840,965 + Pf6N2E2_2401 0.56 +1.9 2,840,965 + Pf6N2E2_2401 0.56 +0.5 2,840,965 + Pf6N2E2_2401 0.56 -0.1 2,840,965 + Pf6N2E2_2401 0.56 +2.5 2,840,966 - Pf6N2E2_2401 0.56 +0.2 2,840,966 - Pf6N2E2_2401 0.56 +2.6 2,840,966 - Pf6N2E2_2401 0.56 -2.2 2,840,966 - Pf6N2E2_2401 0.56 +0.7 2,840,966 - Pf6N2E2_2401 0.56 +0.2 2,840,966 - Pf6N2E2_2401 0.56 -3.6 2,841,079 + Pf6N2E2_2401 0.70 -0.6 2,841,079 + Pf6N2E2_2401 0.70 -0.5 2,841,241 + Pf6N2E2_2401 0.90 -1.4 2,841,241 + Pf6N2E2_2401 0.90 +0.6 2,841,241 + Pf6N2E2_2401 0.90 -1.2 2,841,242 - -1.7 2,841,242 - +0.9 2,841,242 - +0.8 2,841,242 - -1.8 2,841,286 + -0.6 2,841,286 + -0.0 2,841,287 - -0.3 2,841,287 - +0.3 2,841,289 + -0.1 2,841,290 - +0.5 2,841,290 - +1.9 2,841,290 - -0.5 2,841,290 - -2.0 2,841,290 - +0.6 2,841,290 - +1.2 2,841,401 - -2.6 2,841,401 - -0.1 2,841,401 - +1.3 2,841,423 - -2.1 2,841,423 - -0.7 2,841,436 - +0.7 2,841,436 - +0.1 2,841,436 - +0.8 2,841,503 + -0.8 2,841,633 + Pf6N2E2_2402 0.51 +0.0 2,841,633 + Pf6N2E2_2402 0.51 -1.8 2,841,634 - Pf6N2E2_2402 0.52 -0.1 2,841,801 - -2.3 2,841,847 - -1.8 2,841,847 - +1.3 2,841,861 + -0.5 2,841,861 + +0.0 2,841,861 + +1.1 2,841,862 - -1.1 2,841,945 + -0.5 2,841,946 - -1.8 2,841,946 - -0.1 2,841,995 + -2.6 2,841,996 - -2.5 2,842,275 + +0.2 2,842,275 + +2.2 2,842,275 + +0.6 2,842,276 - +0.5 2,842,276 - -0.3 2,842,276 - -0.2 2,842,276 - -0.0 2,842,276 - -1.5 2,842,276 - -0.5
Or see this region's nucleotide sequence