Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2357 and Pf6N2E2_2358 are separated by 713 nucleotides Pf6N2E2_2358 and Pf6N2E2_2359 are separated by 10 nucleotides
Pf6N2E2_2357 - Invasion protein iagA, at 2,790,348 to 2,791,409
_2357
Pf6N2E2_2358 - Type III secretion host injection protein (YopB), at 2,792,123 to 2,793,223
_2358
Pf6N2E2_2359 - hypothetical protein, at 2,793,234 to 2,794,343
_2359
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2791.322 kb on - strand at 2791.337 kb on - strand at 2791.337 kb on - strand at 2791.377 kb on - strand at 2791.377 kb on - strand at 2791.419 kb on + strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.482 kb on + strand at 2791.482 kb on + strand at 2791.608 kb on - strand at 2791.625 kb on + strand at 2791.686 kb on + strand at 2791.728 kb on + strand at 2791.728 kb on + strand at 2791.729 kb on - strand at 2791.736 kb on + strand at 2791.751 kb on + strand at 2791.752 kb on - strand at 2791.752 kb on - strand at 2791.930 kb on - strand at 2791.930 kb on - strand at 2792.014 kb on + strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.056 kb on + strand at 2792.077 kb on + strand at 2792.077 kb on + strand at 2792.077 kb on + strand at 2792.078 kb on - strand at 2792.078 kb on - strand at 2792.078 kb on - strand at 2792.220 kb on + strand at 2792.221 kb on - strand at 2792.296 kb on - strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.468 kb on - strand, within Pf6N2E2_2358 at 2792.504 kb on - strand, within Pf6N2E2_2358 at 2792.542 kb on + strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.559 kb on - strand, within Pf6N2E2_2358 at 2792.630 kb on - strand, within Pf6N2E2_2358 at 2792.633 kb on + strand, within Pf6N2E2_2358 at 2792.634 kb on - strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.698 kb on - strand, within Pf6N2E2_2358 at 2793.126 kb on - strand at 2793.126 kb on - strand at 2793.204 kb on - strand at 2793.410 kb on - strand, within Pf6N2E2_2359 at 2793.561 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2794.112 kb on + strand at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,791,322 - +0.4 2,791,337 - +0.1 2,791,337 - +2.3 2,791,377 - +0.4 2,791,377 - +0.5 2,791,419 + -0.2 2,791,420 - -1.2 2,791,420 - -0.0 2,791,420 - -1.0 2,791,420 - -2.1 2,791,420 - -2.5 2,791,420 - -1.2 2,791,420 - +1.5 2,791,420 - -2.4 2,791,420 - +0.5 2,791,420 - -2.1 2,791,482 + -1.4 2,791,482 + -1.0 2,791,608 - +0.7 2,791,625 + -0.5 2,791,686 + -1.5 2,791,728 + -0.6 2,791,728 + -0.8 2,791,729 - -1.7 2,791,736 + +0.9 2,791,751 + +0.2 2,791,752 - -1.8 2,791,752 - +0.2 2,791,930 - +0.7 2,791,930 - -1.0 2,792,014 + -2.0 2,792,015 - +0.1 2,792,015 - +0.1 2,792,015 - +2.0 2,792,015 - +1.7 2,792,015 - -0.7 2,792,056 + -2.6 2,792,077 + +1.8 2,792,077 + -1.6 2,792,077 + +1.1 2,792,078 - -0.2 2,792,078 - -2.7 2,792,078 - -1.6 2,792,220 + +0.4 2,792,221 - -0.1 2,792,296 - Pf6N2E2_2358 0.16 -1.7 2,792,345 + Pf6N2E2_2358 0.20 -1.8 2,792,345 + Pf6N2E2_2358 0.20 -0.4 2,792,468 - Pf6N2E2_2358 0.31 -0.1 2,792,504 - Pf6N2E2_2358 0.35 +0.2 2,792,542 + Pf6N2E2_2358 0.38 +0.7 2,792,543 - Pf6N2E2_2358 0.38 -1.4 2,792,543 - Pf6N2E2_2358 0.38 -0.5 2,792,559 - Pf6N2E2_2358 0.40 +1.8 2,792,630 - Pf6N2E2_2358 0.46 -0.1 2,792,633 + Pf6N2E2_2358 0.46 -0.7 2,792,634 - Pf6N2E2_2358 0.46 +1.3 2,792,664 + Pf6N2E2_2358 0.49 -0.8 2,792,664 + Pf6N2E2_2358 0.49 +1.6 2,792,664 + Pf6N2E2_2358 0.49 -1.1 2,792,664 + Pf6N2E2_2358 0.49 +0.5 2,792,664 + Pf6N2E2_2358 0.49 +2.6 2,792,665 - Pf6N2E2_2358 0.49 -1.4 2,792,665 - Pf6N2E2_2358 0.49 -2.1 2,792,665 - Pf6N2E2_2358 0.49 -1.2 2,792,665 - Pf6N2E2_2358 0.49 -0.4 2,792,665 - Pf6N2E2_2358 0.49 -1.2 2,792,665 - Pf6N2E2_2358 0.49 +0.3 2,792,698 - Pf6N2E2_2358 0.52 -2.8 2,793,126 - +1.8 2,793,126 - +0.2 2,793,204 - -0.4 2,793,410 - Pf6N2E2_2359 0.16 -2.2 2,793,561 + Pf6N2E2_2359 0.29 +0.3 2,793,667 + Pf6N2E2_2359 0.39 -1.6 2,793,667 + Pf6N2E2_2359 0.39 -1.8 2,793,667 + Pf6N2E2_2359 0.39 +1.4 2,793,667 + Pf6N2E2_2359 0.39 +1.3 2,793,668 - Pf6N2E2_2359 0.39 +2.9 2,793,668 - Pf6N2E2_2359 0.39 +2.5 2,793,934 + Pf6N2E2_2359 0.63 +2.6 2,793,934 + Pf6N2E2_2359 0.63 -1.1 2,793,934 + Pf6N2E2_2359 0.63 -2.7 2,794,112 + -0.2 2,794,115 + Pf6N2E2_2359 0.79 +0.5 2,794,115 + Pf6N2E2_2359 0.79 +1.6 2,794,115 + Pf6N2E2_2359 0.79 +0.5 2,794,115 + Pf6N2E2_2359 0.79 +1.6 2,794,116 - Pf6N2E2_2359 0.79 +0.4 2,794,116 - Pf6N2E2_2359 0.79 -1.5
Or see this region's nucleotide sequence