Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2221 and Pf6N2E2_2222 are separated by 275 nucleotides Pf6N2E2_2222 and Pf6N2E2_2223 are separated by 12 nucleotides Pf6N2E2_2223 and Pf6N2E2_2224 are separated by 100 nucleotides
Pf6N2E2_2221 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 2,635,629 to 2,636,483
_2221
Pf6N2E2_2222 - Murein-DD-endopeptidase (EC 3.4.99.-), at 2,636,759 to 2,637,736
_2222
Pf6N2E2_2223 - hypothetical protein, at 2,637,749 to 2,637,943
_2223
Pf6N2E2_2224 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,638,044 to 2,638,778
_2224
Position (kb)
2636
2637
2638 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2636.627 kb on - strand at 2636.678 kb on + strand at 2636.679 kb on - strand at 2636.679 kb on - strand at 2636.679 kb on - strand at 2636.684 kb on + strand at 2636.684 kb on + strand at 2636.684 kb on + strand at 2636.705 kb on + strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.706 kb on - strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.707 kb on + strand at 2636.708 kb on - strand at 2636.708 kb on - strand at 2636.725 kb on + strand at 2636.726 kb on - strand at 2636.791 kb on + strand at 2637.062 kb on + strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.063 kb on - strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.284 kb on + strand, within Pf6N2E2_2222 at 2637.285 kb on - strand, within Pf6N2E2_2222 at 2637.285 kb on - strand, within Pf6N2E2_2222 at 2637.366 kb on - strand, within Pf6N2E2_2222 at 2637.410 kb on - strand, within Pf6N2E2_2222 at 2637.428 kb on + strand, within Pf6N2E2_2222 at 2637.429 kb on - strand, within Pf6N2E2_2222 at 2637.593 kb on + strand, within Pf6N2E2_2222 at 2637.593 kb on + strand, within Pf6N2E2_2222 at 2637.593 kb on + strand, within Pf6N2E2_2222 at 2637.677 kb on + strand at 2637.677 kb on + strand at 2637.678 kb on - strand at 2637.678 kb on - strand at 2637.678 kb on - strand at 2637.678 kb on - strand at 2637.734 kb on + strand at 2637.735 kb on - strand at 2637.746 kb on + strand at 2637.747 kb on - strand at 2637.750 kb on + strand at 2637.751 kb on - strand at 2637.751 kb on - strand at 2637.777 kb on + strand, within Pf6N2E2_2223 at 2637.777 kb on + strand, within Pf6N2E2_2223 at 2637.923 kb on - strand, within Pf6N2E2_2223 at 2637.989 kb on + strand at 2637.989 kb on + strand at 2637.990 kb on - strand at 2638.163 kb on + strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.164 kb on - strand, within Pf6N2E2_2224 at 2638.266 kb on + strand, within Pf6N2E2_2224 at 2638.267 kb on - strand, within Pf6N2E2_2224 at 2638.289 kb on - strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.342 kb on + strand, within Pf6N2E2_2224 at 2638.343 kb on - strand, within Pf6N2E2_2224 at 2638.412 kb on - strand, within Pf6N2E2_2224 at 2638.427 kb on - strand, within Pf6N2E2_2224 at 2638.448 kb on + strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.449 kb on - strand, within Pf6N2E2_2224 at 2638.567 kb on + strand, within Pf6N2E2_2224 at 2638.568 kb on - strand, within Pf6N2E2_2224 at 2638.599 kb on - strand, within Pf6N2E2_2224 at 2638.626 kb on + strand, within Pf6N2E2_2224 at 2638.627 kb on - strand, within Pf6N2E2_2224 at 2638.627 kb on - strand, within Pf6N2E2_2224 at 2638.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,636,627 - +0.9 2,636,678 + -1.1 2,636,679 - -0.3 2,636,679 - +0.2 2,636,679 - +0.7 2,636,684 + -2.7 2,636,684 + -0.6 2,636,684 + +0.1 2,636,705 + -0.8 2,636,706 - +1.1 2,636,706 - +1.3 2,636,706 - -1.2 2,636,706 - -3.0 2,636,706 - -0.2 2,636,706 - -1.0 2,636,707 + +0.8 2,636,707 + -0.9 2,636,707 + +1.4 2,636,707 + +0.7 2,636,708 - +0.8 2,636,708 - -1.1 2,636,725 + +0.1 2,636,726 - -2.9 2,636,791 + -1.3 2,637,062 + Pf6N2E2_2222 0.31 +1.7 2,637,063 - Pf6N2E2_2222 0.31 -3.1 2,637,063 - Pf6N2E2_2222 0.31 -1.0 2,637,063 - Pf6N2E2_2222 0.31 -0.2 2,637,063 - Pf6N2E2_2222 0.31 -1.3 2,637,284 + Pf6N2E2_2222 0.54 +0.6 2,637,284 + Pf6N2E2_2222 0.54 +1.1 2,637,284 + Pf6N2E2_2222 0.54 +1.8 2,637,284 + Pf6N2E2_2222 0.54 +2.4 2,637,284 + Pf6N2E2_2222 0.54 +0.7 2,637,284 + Pf6N2E2_2222 0.54 +0.5 2,637,284 + Pf6N2E2_2222 0.54 +1.1 2,637,285 - Pf6N2E2_2222 0.54 -0.5 2,637,285 - Pf6N2E2_2222 0.54 +0.1 2,637,366 - Pf6N2E2_2222 0.62 -0.4 2,637,410 - Pf6N2E2_2222 0.67 -0.4 2,637,428 + Pf6N2E2_2222 0.68 -0.6 2,637,429 - Pf6N2E2_2222 0.69 +1.1 2,637,593 + Pf6N2E2_2222 0.85 +0.2 2,637,593 + Pf6N2E2_2222 0.85 +0.8 2,637,593 + Pf6N2E2_2222 0.85 -0.5 2,637,677 + +1.3 2,637,677 + -0.2 2,637,678 - +1.3 2,637,678 - -2.3 2,637,678 - +0.2 2,637,678 - +0.8 2,637,734 + -0.8 2,637,735 - -1.7 2,637,746 + +1.6 2,637,747 - +1.0 2,637,750 + -0.2 2,637,751 - -1.7 2,637,751 - +0.1 2,637,777 + Pf6N2E2_2223 0.14 +1.2 2,637,777 + Pf6N2E2_2223 0.14 +0.3 2,637,923 - Pf6N2E2_2223 0.89 +0.5 2,637,989 + -2.3 2,637,989 + -0.3 2,637,990 - +1.8 2,638,163 + Pf6N2E2_2224 0.16 -0.7 2,638,164 - Pf6N2E2_2224 0.16 +1.9 2,638,164 - Pf6N2E2_2224 0.16 -0.6 2,638,164 - Pf6N2E2_2224 0.16 +0.6 2,638,164 - Pf6N2E2_2224 0.16 +1.7 2,638,164 - Pf6N2E2_2224 0.16 +1.7 2,638,266 + Pf6N2E2_2224 0.30 -1.5 2,638,267 - Pf6N2E2_2224 0.30 -1.3 2,638,289 - Pf6N2E2_2224 0.33 -0.9 2,638,342 + Pf6N2E2_2224 0.41 -2.6 2,638,342 + Pf6N2E2_2224 0.41 -1.5 2,638,342 + Pf6N2E2_2224 0.41 -3.2 2,638,343 - Pf6N2E2_2224 0.41 -0.7 2,638,412 - Pf6N2E2_2224 0.50 -2.8 2,638,427 - Pf6N2E2_2224 0.52 -2.7 2,638,448 + Pf6N2E2_2224 0.55 -3.1 2,638,449 - Pf6N2E2_2224 0.55 +0.5 2,638,449 - Pf6N2E2_2224 0.55 -1.9 2,638,449 - Pf6N2E2_2224 0.55 +1.9 2,638,567 + Pf6N2E2_2224 0.71 -1.3 2,638,568 - Pf6N2E2_2224 0.71 -2.5 2,638,599 - Pf6N2E2_2224 0.76 +1.7 2,638,626 + Pf6N2E2_2224 0.79 -2.7 2,638,627 - Pf6N2E2_2224 0.79 -1.0 2,638,627 - Pf6N2E2_2224 0.79 -0.1 2,638,709 + -2.6
Or see this region's nucleotide sequence