Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1988 and Pf6N2E2_1989 are separated by 118 nucleotides Pf6N2E2_1989 and Pf6N2E2_1990 are separated by 404 nucleotides Pf6N2E2_1990 and Pf6N2E2_1991 are separated by 257 nucleotides Pf6N2E2_1991 and Pf6N2E2_1992 are separated by 177 nucleotides Pf6N2E2_1992 and Pf6N2E2_1993 are separated by 160 nucleotides
Pf6N2E2_1988 - hypothetical protein, at 2,354,318 to 2,354,551
_1988
Pf6N2E2_1989 - hypothetical protein, at 2,354,670 to 2,354,927
_1989
Pf6N2E2_1990 - Possible exported protein, at 2,355,332 to 2,356,015
_1990
Pf6N2E2_1991 - hypothetical protein, at 2,356,273 to 2,356,407
_1991
Pf6N2E2_1992 - hypothetical protein, at 2,356,585 to 2,356,728
_1992
Pf6N2E2_1993 - putative exported protein, at 2,356,889 to 2,358,316
_1993
Position (kb)
2355
2356
2357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2354.519 kb on + strand, within Pf6N2E2_1988 at 2354.724 kb on + strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.800 kb on - strand, within Pf6N2E2_1989 at 2355.140 kb on - strand at 2355.252 kb on + strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.460 kb on - strand, within Pf6N2E2_1990 at 2355.503 kb on - strand, within Pf6N2E2_1990 at 2355.548 kb on + strand, within Pf6N2E2_1990 at 2355.549 kb on - strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.627 kb on - strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.685 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.769 kb on - strand, within Pf6N2E2_1990 at 2355.793 kb on + strand, within Pf6N2E2_1990 at 2355.873 kb on + strand, within Pf6N2E2_1990 at 2355.977 kb on - strand at 2356.088 kb on + strand at 2356.089 kb on - strand at 2356.162 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.202 kb on + strand at 2356.232 kb on - strand at 2356.232 kb on - strand at 2356.326 kb on - strand, within Pf6N2E2_1991 at 2356.438 kb on - strand at 2356.439 kb on + strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.464 kb on - strand at 2356.504 kb on - strand at 2356.511 kb on + strand at 2356.511 kb on + strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.568 kb on + strand at 2356.569 kb on - strand at 2356.579 kb on - strand at 2356.627 kb on + strand, within Pf6N2E2_1992
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,354,519 + Pf6N2E2_1988 0.86 +1.6 2,354,724 + Pf6N2E2_1989 0.21 +1.2 2,354,725 - Pf6N2E2_1989 0.21 -1.6 2,354,725 - Pf6N2E2_1989 0.21 +0.5 2,354,800 - Pf6N2E2_1989 0.50 -1.4 2,355,140 - -1.5 2,355,252 + -2.0 2,355,294 - -1.4 2,355,294 - +0.6 2,355,294 - +1.6 2,355,460 - Pf6N2E2_1990 0.19 -1.2 2,355,503 - Pf6N2E2_1990 0.25 -1.0 2,355,548 + Pf6N2E2_1990 0.32 +0.8 2,355,549 - Pf6N2E2_1990 0.32 -1.3 2,355,596 + Pf6N2E2_1990 0.39 +1.6 2,355,596 + Pf6N2E2_1990 0.39 -0.1 2,355,627 - Pf6N2E2_1990 0.43 -2.4 2,355,665 + Pf6N2E2_1990 0.49 +2.0 2,355,665 + Pf6N2E2_1990 0.49 -1.5 2,355,684 + Pf6N2E2_1990 0.51 +1.7 2,355,684 + Pf6N2E2_1990 0.51 +2.2 2,355,685 - Pf6N2E2_1990 0.52 +2.4 2,355,717 - Pf6N2E2_1990 0.56 -2.2 2,355,717 - Pf6N2E2_1990 0.56 -1.7 2,355,717 - Pf6N2E2_1990 0.56 +2.4 2,355,769 - Pf6N2E2_1990 0.64 +0.5 2,355,793 + Pf6N2E2_1990 0.67 -2.7 2,355,873 + Pf6N2E2_1990 0.79 -1.4 2,355,977 - +0.9 2,356,088 + -0.6 2,356,089 - +1.2 2,356,162 - +0.6 2,356,167 - +1.6 2,356,167 - +0.6 2,356,167 - +2.0 2,356,167 - -1.0 2,356,202 + +1.4 2,356,232 - +1.0 2,356,232 - -2.0 2,356,326 - Pf6N2E2_1991 0.39 +0.9 2,356,438 - -2.1 2,356,439 + -0.8 2,356,440 - -1.3 2,356,440 - -1.4 2,356,440 - -1.2 2,356,464 - +0.8 2,356,504 - -2.2 2,356,511 + -1.8 2,356,511 + +1.4 2,356,512 - +0.9 2,356,512 - -1.0 2,356,512 - +1.2 2,356,512 - +0.6 2,356,568 + +0.6 2,356,569 - -0.2 2,356,579 - -1.7 2,356,627 + Pf6N2E2_1992 0.29 +1.3
Or see this region's nucleotide sequence