Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1941 and Pf6N2E2_1942 are separated by 322 nucleotides Pf6N2E2_1942 and Pf6N2E2_1943 are separated by 267 nucleotides Pf6N2E2_1943 and Pf6N2E2_1944 are separated by 72 nucleotides
Pf6N2E2_1941 - TcuB: works with TcuA to oxidize tricarballylate to cis-aconitate, at 2,302,382 to 2,303,539
_1941
Pf6N2E2_1942 - Glucose-selective porin OprB, at 2,303,862 to 2,305,190
_1942
Pf6N2E2_1943 - Coenzyme PQQ synthesis protein A, at 2,305,458 to 2,305,598
_1943
Pf6N2E2_1944 - Coenzyme PQQ synthesis protein B, at 2,305,671 to 2,306,582
_1944
Position (kb)
2303
2304
2305
2306 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2303.009 kb on + strand, within Pf6N2E2_1941 at 2303.010 kb on - strand, within Pf6N2E2_1941 at 2303.010 kb on - strand, within Pf6N2E2_1941 at 2303.096 kb on + strand, within Pf6N2E2_1941 at 2303.097 kb on - strand, within Pf6N2E2_1941 at 2303.217 kb on + strand, within Pf6N2E2_1941 at 2303.217 kb on + strand, within Pf6N2E2_1941 at 2303.217 kb on + strand, within Pf6N2E2_1941 at 2303.217 kb on + strand, within Pf6N2E2_1941 at 2303.680 kb on + strand at 2303.681 kb on - strand at 2303.894 kb on + strand at 2303.894 kb on + strand at 2303.995 kb on + strand, within Pf6N2E2_1942 at 2304.023 kb on + strand, within Pf6N2E2_1942 at 2304.217 kb on + strand, within Pf6N2E2_1942 at 2304.263 kb on - strand, within Pf6N2E2_1942 at 2304.263 kb on - strand, within Pf6N2E2_1942 at 2304.290 kb on + strand, within Pf6N2E2_1942 at 2304.290 kb on + strand, within Pf6N2E2_1942 at 2304.291 kb on - strand, within Pf6N2E2_1942 at 2304.291 kb on - strand, within Pf6N2E2_1942 at 2304.447 kb on + strand, within Pf6N2E2_1942 at 2304.447 kb on + strand, within Pf6N2E2_1942 at 2304.447 kb on + strand, within Pf6N2E2_1942 at 2304.448 kb on - strand, within Pf6N2E2_1942 at 2304.507 kb on + strand, within Pf6N2E2_1942 at 2304.508 kb on - strand, within Pf6N2E2_1942 at 2304.508 kb on - strand, within Pf6N2E2_1942 at 2304.543 kb on + strand, within Pf6N2E2_1942 at 2304.594 kb on - strand, within Pf6N2E2_1942 at 2304.601 kb on + strand, within Pf6N2E2_1942 at 2304.620 kb on + strand, within Pf6N2E2_1942 at 2304.673 kb on - strand, within Pf6N2E2_1942 at 2304.675 kb on + strand, within Pf6N2E2_1942 at 2304.721 kb on + strand, within Pf6N2E2_1942 at 2304.721 kb on + strand, within Pf6N2E2_1942 at 2304.722 kb on - strand, within Pf6N2E2_1942 at 2304.822 kb on + strand, within Pf6N2E2_1942 at 2304.851 kb on + strand, within Pf6N2E2_1942 at 2304.851 kb on + strand, within Pf6N2E2_1942 at 2304.852 kb on - strand, within Pf6N2E2_1942 at 2304.874 kb on + strand, within Pf6N2E2_1942 at 2304.883 kb on - strand, within Pf6N2E2_1942 at 2304.886 kb on - strand, within Pf6N2E2_1942 at 2305.062 kb on + strand at 2305.062 kb on + strand at 2305.063 kb on - strand at 2305.065 kb on + strand at 2305.066 kb on - strand at 2305.066 kb on - strand at 2305.117 kb on + strand at 2305.118 kb on - strand at 2305.188 kb on + strand at 2305.202 kb on + strand at 2305.203 kb on - strand at 2305.343 kb on + strand at 2305.343 kb on + strand at 2305.404 kb on + strand at 2305.405 kb on - strand at 2305.405 kb on - strand at 2305.406 kb on + strand at 2305.406 kb on + strand at 2305.406 kb on + strand at 2305.407 kb on - strand at 2305.429 kb on - strand at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.482 kb on - strand, within Pf6N2E2_1943 at 2305.482 kb on - strand, within Pf6N2E2_1943 at 2305.485 kb on + strand, within Pf6N2E2_1943 at 2305.511 kb on + strand, within Pf6N2E2_1943 at 2305.511 kb on + strand, within Pf6N2E2_1943 at 2305.512 kb on - strand, within Pf6N2E2_1943 at 2305.512 kb on - strand, within Pf6N2E2_1943 at 2305.517 kb on + strand, within Pf6N2E2_1943 at 2305.517 kb on + strand, within Pf6N2E2_1943 at 2305.518 kb on - strand, within Pf6N2E2_1943 at 2305.545 kb on + strand, within Pf6N2E2_1943 at 2305.546 kb on - strand, within Pf6N2E2_1943 at 2305.581 kb on + strand, within Pf6N2E2_1943 at 2305.646 kb on + strand at 2305.647 kb on - strand at 2305.687 kb on + strand at 2305.688 kb on - strand at 2305.688 kb on - strand at 2305.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,303,009 + Pf6N2E2_1941 0.54 +1.5 2,303,010 - Pf6N2E2_1941 0.54 +0.6 2,303,010 - Pf6N2E2_1941 0.54 -1.1 2,303,096 + Pf6N2E2_1941 0.62 -0.8 2,303,097 - Pf6N2E2_1941 0.62 -0.1 2,303,217 + Pf6N2E2_1941 0.72 -0.2 2,303,217 + Pf6N2E2_1941 0.72 +1.3 2,303,217 + Pf6N2E2_1941 0.72 +1.9 2,303,217 + Pf6N2E2_1941 0.72 -1.1 2,303,680 + +1.3 2,303,681 - -0.6 2,303,894 + +0.6 2,303,894 + +1.7 2,303,995 + Pf6N2E2_1942 0.10 -0.6 2,304,023 + Pf6N2E2_1942 0.12 -0.8 2,304,217 + Pf6N2E2_1942 0.27 -1.0 2,304,263 - Pf6N2E2_1942 0.30 +0.0 2,304,263 - Pf6N2E2_1942 0.30 +2.0 2,304,290 + Pf6N2E2_1942 0.32 -2.6 2,304,290 + Pf6N2E2_1942 0.32 +2.1 2,304,291 - Pf6N2E2_1942 0.32 -2.2 2,304,291 - Pf6N2E2_1942 0.32 +0.2 2,304,447 + Pf6N2E2_1942 0.44 +1.9 2,304,447 + Pf6N2E2_1942 0.44 +0.6 2,304,447 + Pf6N2E2_1942 0.44 -0.7 2,304,448 - Pf6N2E2_1942 0.44 -0.6 2,304,507 + Pf6N2E2_1942 0.49 -2.6 2,304,508 - Pf6N2E2_1942 0.49 +0.4 2,304,508 - Pf6N2E2_1942 0.49 -2.2 2,304,543 + Pf6N2E2_1942 0.51 +0.6 2,304,594 - Pf6N2E2_1942 0.55 +0.9 2,304,601 + Pf6N2E2_1942 0.56 -2.4 2,304,620 + Pf6N2E2_1942 0.57 +0.8 2,304,673 - Pf6N2E2_1942 0.61 -0.8 2,304,675 + Pf6N2E2_1942 0.61 +0.6 2,304,721 + Pf6N2E2_1942 0.65 +0.1 2,304,721 + Pf6N2E2_1942 0.65 -2.2 2,304,722 - Pf6N2E2_1942 0.65 -1.7 2,304,822 + Pf6N2E2_1942 0.72 -1.6 2,304,851 + Pf6N2E2_1942 0.74 -1.6 2,304,851 + Pf6N2E2_1942 0.74 +0.2 2,304,852 - Pf6N2E2_1942 0.74 -2.0 2,304,874 + Pf6N2E2_1942 0.76 -1.2 2,304,883 - Pf6N2E2_1942 0.77 -1.4 2,304,886 - Pf6N2E2_1942 0.77 +0.7 2,305,062 + -0.6 2,305,062 + -2.5 2,305,063 - +1.6 2,305,065 + -0.0 2,305,066 - -1.1 2,305,066 - +0.7 2,305,117 + +0.9 2,305,118 - -1.7 2,305,188 + -0.8 2,305,202 + +0.2 2,305,203 - +0.0 2,305,343 + -1.3 2,305,343 + +1.3 2,305,404 + +0.3 2,305,405 - -1.8 2,305,405 - +2.8 2,305,406 + -2.9 2,305,406 + +1.6 2,305,406 + -0.3 2,305,407 - -0.6 2,305,429 - +0.4 2,305,481 + Pf6N2E2_1943 0.16 -0.4 2,305,481 + Pf6N2E2_1943 0.16 -2.2 2,305,481 + Pf6N2E2_1943 0.16 +1.0 2,305,482 - Pf6N2E2_1943 0.17 +1.6 2,305,482 - Pf6N2E2_1943 0.17 -0.6 2,305,485 + Pf6N2E2_1943 0.19 +1.6 2,305,511 + Pf6N2E2_1943 0.38 -2.8 2,305,511 + Pf6N2E2_1943 0.38 -0.5 2,305,512 - Pf6N2E2_1943 0.38 +0.8 2,305,512 - Pf6N2E2_1943 0.38 +0.0 2,305,517 + Pf6N2E2_1943 0.42 -0.6 2,305,517 + Pf6N2E2_1943 0.42 -1.6 2,305,518 - Pf6N2E2_1943 0.43 -0.7 2,305,545 + Pf6N2E2_1943 0.62 +1.5 2,305,546 - Pf6N2E2_1943 0.62 +0.5 2,305,581 + Pf6N2E2_1943 0.87 +0.5 2,305,646 + +0.0 2,305,647 - -2.6 2,305,687 + +0.4 2,305,688 - -1.5 2,305,688 - -3.1 2,305,688 - +1.0
Or see this region's nucleotide sequence