Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1851

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1850 and Pf6N2E2_1851 are separated by 63 nucleotidesPf6N2E2_1851 and Pf6N2E2_1852 are separated by 389 nucleotides Pf6N2E2_1850 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,189,848 to 2,191,995 _1850 Pf6N2E2_1851 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,192,059 to 2,193,267 _1851 Pf6N2E2_1852 - Regulatory protein, LuxR:Tetratricopeptide TPR_4, at 2,193,657 to 2,196,332 _1852 Position (kb) 2192 2193 2194Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2191.364 kb on - strand, within Pf6N2E2_1850at 2191.635 kb on + strand, within Pf6N2E2_1850at 2191.636 kb on - strand, within Pf6N2E2_1850at 2191.870 kb on + strandat 2191.905 kb on - strandat 2191.995 kb on + strandat 2191.995 kb on + strandat 2191.995 kb on + strandat 2191.995 kb on + strandat 2191.995 kb on + strandat 2191.996 kb on - strandat 2192.451 kb on + strand, within Pf6N2E2_1851at 2192.505 kb on + strand, within Pf6N2E2_1851at 2192.547 kb on - strand, within Pf6N2E2_1851at 2192.550 kb on + strand, within Pf6N2E2_1851at 2192.550 kb on + strand, within Pf6N2E2_1851at 2192.550 kb on + strand, within Pf6N2E2_1851at 2192.551 kb on - strand, within Pf6N2E2_1851at 2192.560 kb on + strand, within Pf6N2E2_1851at 2192.561 kb on - strand, within Pf6N2E2_1851at 2192.571 kb on - strand, within Pf6N2E2_1851at 2192.581 kb on - strand, within Pf6N2E2_1851at 2193.011 kb on - strand, within Pf6N2E2_1851at 2193.127 kb on - strand, within Pf6N2E2_1851at 2193.137 kb on + strand, within Pf6N2E2_1851at 2193.194 kb on + strandat 2193.246 kb on - strandat 2193.246 kb on - strandat 2193.254 kb on - strandat 2193.310 kb on + strandat 2193.311 kb on - strandat 2193.311 kb on - strandat 2193.376 kb on - strandat 2193.376 kb on - strandat 2193.471 kb on + strandat 2193.504 kb on + strandat 2193.504 kb on + strandat 2193.504 kb on + strandat 2193.505 kb on - strandat 2193.553 kb on + strandat 2193.599 kb on + strandat 2193.600 kb on - strandat 2193.600 kb on - strandat 2193.604 kb on + strandat 2193.604 kb on + strandat 2193.605 kb on - strandat 2193.685 kb on - strandat 2193.949 kb on + strand, within Pf6N2E2_1852at 2193.950 kb on - strand, within Pf6N2E2_1852at 2194.142 kb on - strand, within Pf6N2E2_1852at 2194.142 kb on - strand, within Pf6N2E2_1852at 2194.142 kb on - strand, within Pf6N2E2_1852at 2194.148 kb on - strand, within Pf6N2E2_1852at 2194.148 kb on - strand, within Pf6N2E2_1852at 2194.148 kb on - strand, within Pf6N2E2_1852at 2194.171 kb on + strand, within Pf6N2E2_1852at 2194.171 kb on + strand, within Pf6N2E2_1852at 2194.171 kb on + strand, within Pf6N2E2_1852at 2194.172 kb on - strand, within Pf6N2E2_1852

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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2,191,364 - Pf6N2E2_1850 0.71 +0.5
2,191,635 + Pf6N2E2_1850 0.83 +1.3
2,191,636 - Pf6N2E2_1850 0.83 -0.7
2,191,870 + -1.7
2,191,905 - +0.5
2,191,995 + +2.4
2,191,995 + -2.0
2,191,995 + +0.5
2,191,995 + +1.1
2,191,995 + +1.6
2,191,996 - +1.8
2,192,451 + Pf6N2E2_1851 0.32 -2.7
2,192,505 + Pf6N2E2_1851 0.37 +0.4
2,192,547 - Pf6N2E2_1851 0.40 -2.0
2,192,550 + Pf6N2E2_1851 0.41 +0.2
2,192,550 + Pf6N2E2_1851 0.41 -0.4
2,192,550 + Pf6N2E2_1851 0.41 -3.0
2,192,551 - Pf6N2E2_1851 0.41 -0.0
2,192,560 + Pf6N2E2_1851 0.41 -1.8
2,192,561 - Pf6N2E2_1851 0.42 -2.5
2,192,571 - Pf6N2E2_1851 0.42 -0.5
2,192,581 - Pf6N2E2_1851 0.43 -0.3
2,193,011 - Pf6N2E2_1851 0.79 +1.9
2,193,127 - Pf6N2E2_1851 0.88 +1.8
2,193,137 + Pf6N2E2_1851 0.89 -2.5
2,193,194 + +1.2
2,193,246 - +1.0
2,193,246 - +0.6
2,193,254 - -2.4
2,193,310 + -0.4
2,193,311 - +2.1
2,193,311 - +2.5
2,193,376 - -3.1
2,193,376 - -0.5
2,193,471 + +0.3
2,193,504 + -1.7
2,193,504 + +1.2
2,193,504 + +0.5
2,193,505 - +0.3
2,193,553 + +1.0
2,193,599 + +0.6
2,193,600 - +1.2
2,193,600 - -0.4
2,193,604 + -1.3
2,193,604 + +0.3
2,193,605 - -0.0
2,193,685 - -1.0
2,193,949 + Pf6N2E2_1852 0.11 -2.3
2,193,950 - Pf6N2E2_1852 0.11 -0.7
2,194,142 - Pf6N2E2_1852 0.18 -3.6
2,194,142 - Pf6N2E2_1852 0.18 -3.1
2,194,142 - Pf6N2E2_1852 0.18 +1.0
2,194,148 - Pf6N2E2_1852 0.18 -0.2
2,194,148 - Pf6N2E2_1852 0.18 +1.4
2,194,148 - Pf6N2E2_1852 0.18 +0.8
2,194,171 + Pf6N2E2_1852 0.19 -0.6
2,194,171 + Pf6N2E2_1852 0.19 +1.0
2,194,171 + Pf6N2E2_1852 0.19 +0.3
2,194,172 - Pf6N2E2_1852 0.19 -1.5

Or see this region's nucleotide sequence