Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1850 and Pf6N2E2_1851 are separated by 63 nucleotides Pf6N2E2_1851 and Pf6N2E2_1852 are separated by 389 nucleotides
Pf6N2E2_1850 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,189,848 to 2,191,995
_1850
Pf6N2E2_1851 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,192,059 to 2,193,267
_1851
Pf6N2E2_1852 - Regulatory protein, LuxR:Tetratricopeptide TPR_4, at 2,193,657 to 2,196,332
_1852
Position (kb)
2192
2193
2194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2191.364 kb on - strand, within Pf6N2E2_1850 at 2191.635 kb on + strand, within Pf6N2E2_1850 at 2191.636 kb on - strand, within Pf6N2E2_1850 at 2191.870 kb on + strand at 2191.905 kb on - strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.996 kb on - strand at 2192.451 kb on + strand, within Pf6N2E2_1851 at 2192.505 kb on + strand, within Pf6N2E2_1851 at 2192.547 kb on - strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.551 kb on - strand, within Pf6N2E2_1851 at 2192.560 kb on + strand, within Pf6N2E2_1851 at 2192.561 kb on - strand, within Pf6N2E2_1851 at 2192.571 kb on - strand, within Pf6N2E2_1851 at 2192.581 kb on - strand, within Pf6N2E2_1851 at 2193.011 kb on - strand, within Pf6N2E2_1851 at 2193.127 kb on - strand, within Pf6N2E2_1851 at 2193.137 kb on + strand, within Pf6N2E2_1851 at 2193.194 kb on + strand at 2193.246 kb on - strand at 2193.246 kb on - strand at 2193.254 kb on - strand at 2193.310 kb on + strand at 2193.311 kb on - strand at 2193.311 kb on - strand at 2193.376 kb on - strand at 2193.376 kb on - strand at 2193.471 kb on + strand at 2193.504 kb on + strand at 2193.504 kb on + strand at 2193.504 kb on + strand at 2193.505 kb on - strand at 2193.553 kb on + strand at 2193.599 kb on + strand at 2193.600 kb on - strand at 2193.600 kb on - strand at 2193.604 kb on + strand at 2193.604 kb on + strand at 2193.605 kb on - strand at 2193.685 kb on - strand at 2193.949 kb on + strand, within Pf6N2E2_1852 at 2193.950 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.172 kb on - strand, within Pf6N2E2_1852
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 2,191,364 - Pf6N2E2_1850 0.71 +0.5 2,191,635 + Pf6N2E2_1850 0.83 +1.3 2,191,636 - Pf6N2E2_1850 0.83 -0.7 2,191,870 + -1.7 2,191,905 - +0.5 2,191,995 + +2.4 2,191,995 + -2.0 2,191,995 + +0.5 2,191,995 + +1.1 2,191,995 + +1.6 2,191,996 - +1.8 2,192,451 + Pf6N2E2_1851 0.32 -2.7 2,192,505 + Pf6N2E2_1851 0.37 +0.4 2,192,547 - Pf6N2E2_1851 0.40 -2.0 2,192,550 + Pf6N2E2_1851 0.41 +0.2 2,192,550 + Pf6N2E2_1851 0.41 -0.4 2,192,550 + Pf6N2E2_1851 0.41 -3.0 2,192,551 - Pf6N2E2_1851 0.41 -0.0 2,192,560 + Pf6N2E2_1851 0.41 -1.8 2,192,561 - Pf6N2E2_1851 0.42 -2.5 2,192,571 - Pf6N2E2_1851 0.42 -0.5 2,192,581 - Pf6N2E2_1851 0.43 -0.3 2,193,011 - Pf6N2E2_1851 0.79 +1.9 2,193,127 - Pf6N2E2_1851 0.88 +1.8 2,193,137 + Pf6N2E2_1851 0.89 -2.5 2,193,194 + +1.2 2,193,246 - +1.0 2,193,246 - +0.6 2,193,254 - -2.4 2,193,310 + -0.4 2,193,311 - +2.1 2,193,311 - +2.5 2,193,376 - -3.1 2,193,376 - -0.5 2,193,471 + +0.3 2,193,504 + -1.7 2,193,504 + +1.2 2,193,504 + +0.5 2,193,505 - +0.3 2,193,553 + +1.0 2,193,599 + +0.6 2,193,600 - +1.2 2,193,600 - -0.4 2,193,604 + -1.3 2,193,604 + +0.3 2,193,605 - -0.0 2,193,685 - -1.0 2,193,949 + Pf6N2E2_1852 0.11 -2.3 2,193,950 - Pf6N2E2_1852 0.11 -0.7 2,194,142 - Pf6N2E2_1852 0.18 -3.6 2,194,142 - Pf6N2E2_1852 0.18 -3.1 2,194,142 - Pf6N2E2_1852 0.18 +1.0 2,194,148 - Pf6N2E2_1852 0.18 -0.2 2,194,148 - Pf6N2E2_1852 0.18 +1.4 2,194,148 - Pf6N2E2_1852 0.18 +0.8 2,194,171 + Pf6N2E2_1852 0.19 -0.6 2,194,171 + Pf6N2E2_1852 0.19 +1.0 2,194,171 + Pf6N2E2_1852 0.19 +0.3 2,194,172 - Pf6N2E2_1852 0.19 -1.5
Or see this region's nucleotide sequence