Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_148

Experiment: Hydroxylamine 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_147 and Pf6N2E2_148 are separated by 15 nucleotidesPf6N2E2_148 and Pf6N2E2_149 are separated by 274 nucleotides Pf6N2E2_147 - Outer membrane component of tripartite multidrug resistance system, at 201,559 to 203,013 _147 Pf6N2E2_148 - Membrane fusion component of tripartite multidrug resistance system, at 203,029 to 204,225 _148 Pf6N2E2_149 - Inner membrane component of tripartite multidrug resistance system, at 204,500 to 206,029 _149 Position (kb) 203 204 205Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 202.100 kb on - strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.125 kb on + strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.126 kb on - strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.129 kb on + strand, within Pf6N2E2_147at 202.130 kb on - strand, within Pf6N2E2_147at 202.217 kb on - strand, within Pf6N2E2_147at 202.498 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.681 kb on + strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.682 kb on - strand, within Pf6N2E2_147at 202.820 kb on - strand, within Pf6N2E2_147at 202.840 kb on + strand, within Pf6N2E2_147at 203.007 kb on + strandat 203.008 kb on - strandat 203.008 kb on - strandat 203.021 kb on + strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.022 kb on - strandat 203.161 kb on + strand, within Pf6N2E2_148at 203.161 kb on + strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.162 kb on - strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.194 kb on + strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.195 kb on - strand, within Pf6N2E2_148at 203.383 kb on + strand, within Pf6N2E2_148at 203.384 kb on - strand, within Pf6N2E2_148at 203.676 kb on + strand, within Pf6N2E2_148at 203.677 kb on - strand, within Pf6N2E2_148at 203.702 kb on - strand, within Pf6N2E2_148at 203.863 kb on + strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.882 kb on - strand, within Pf6N2E2_148at 203.936 kb on + strand, within Pf6N2E2_148at 204.137 kb on - strandat 204.137 kb on - strandat 204.137 kb on - strandat 204.703 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.791 kb on - strand, within Pf6N2E2_149at 204.828 kb on + strand, within Pf6N2E2_149at 204.829 kb on - strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149

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Per-strain Table

Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M
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202,100 - Pf6N2E2_147 0.37 +1.6
202,125 + Pf6N2E2_147 0.39 +1.5
202,125 + Pf6N2E2_147 0.39 -0.2
202,125 + Pf6N2E2_147 0.39 -0.6
202,125 + Pf6N2E2_147 0.39 -1.3
202,126 - Pf6N2E2_147 0.39 +0.4
202,126 - Pf6N2E2_147 0.39 +0.7
202,126 - Pf6N2E2_147 0.39 -0.8
202,126 - Pf6N2E2_147 0.39 +2.1
202,126 - Pf6N2E2_147 0.39 +1.8
202,126 - Pf6N2E2_147 0.39 +0.8
202,126 - Pf6N2E2_147 0.39 -0.5
202,129 + Pf6N2E2_147 0.39 +1.4
202,129 + Pf6N2E2_147 0.39 +0.1
202,129 + Pf6N2E2_147 0.39 +0.2
202,129 + Pf6N2E2_147 0.39 -3.3
202,130 - Pf6N2E2_147 0.39 -1.5
202,217 - Pf6N2E2_147 0.45 +1.4
202,498 + Pf6N2E2_147 0.65 -1.9
202,681 + Pf6N2E2_147 0.77 -1.7
202,681 + Pf6N2E2_147 0.77 -2.5
202,681 + Pf6N2E2_147 0.77 -0.4
202,682 - Pf6N2E2_147 0.77 +1.0
202,682 - Pf6N2E2_147 0.77 +0.4
202,682 - Pf6N2E2_147 0.77 -1.5
202,682 - Pf6N2E2_147 0.77 -0.5
202,820 - Pf6N2E2_147 0.87 -0.8
202,840 + Pf6N2E2_147 0.88 -0.1
203,007 + +1.2
203,008 - +3.4
203,008 - +3.3
203,021 + +1.9
203,022 - -0.2
203,022 - +3.0
203,022 - +0.6
203,022 - -1.4
203,161 + Pf6N2E2_148 0.11 +0.2
203,161 + Pf6N2E2_148 0.11 +0.6
203,162 - Pf6N2E2_148 0.11 -0.3
203,162 - Pf6N2E2_148 0.11 +5.9
203,162 - Pf6N2E2_148 0.11 -3.7
203,162 - Pf6N2E2_148 0.11 +1.7
203,162 - Pf6N2E2_148 0.11 +0.7
203,194 + Pf6N2E2_148 0.14 +1.2
203,194 + Pf6N2E2_148 0.14 +2.0
203,194 + Pf6N2E2_148 0.14 +1.5
203,195 - Pf6N2E2_148 0.14 +1.2
203,195 - Pf6N2E2_148 0.14 -0.3
203,195 - Pf6N2E2_148 0.14 -0.6
203,195 - Pf6N2E2_148 0.14 -3.0
203,195 - Pf6N2E2_148 0.14 +1.5
203,195 - Pf6N2E2_148 0.14 +1.0
203,383 + Pf6N2E2_148 0.30 +1.8
203,384 - Pf6N2E2_148 0.30 +0.9
203,676 + Pf6N2E2_148 0.54 -1.8
203,677 - Pf6N2E2_148 0.54 +1.0
203,702 - Pf6N2E2_148 0.56 +1.3
203,863 + Pf6N2E2_148 0.70 -1.3
203,864 - Pf6N2E2_148 0.70 +2.8
203,864 - Pf6N2E2_148 0.70 +0.2
203,881 + Pf6N2E2_148 0.71 +0.4
203,881 + Pf6N2E2_148 0.71 -0.4
203,881 + Pf6N2E2_148 0.71 +1.1
203,882 - Pf6N2E2_148 0.71 +2.1
203,936 + Pf6N2E2_148 0.76 +1.7
204,137 - -0.2
204,137 - +0.8
204,137 - -0.9
204,703 + Pf6N2E2_149 0.13 -2.9
204,790 + Pf6N2E2_149 0.19 +0.6
204,790 + Pf6N2E2_149 0.19 -0.2
204,791 - Pf6N2E2_149 0.19 -1.8
204,828 + Pf6N2E2_149 0.21 -2.4
204,829 - Pf6N2E2_149 0.22 +1.2
204,992 + Pf6N2E2_149 0.32 +1.8
204,992 + Pf6N2E2_149 0.32 +1.3
204,992 + Pf6N2E2_149 0.32 -1.6
204,992 + Pf6N2E2_149 0.32 -0.3
204,992 + Pf6N2E2_149 0.32 -0.2
204,992 + Pf6N2E2_149 0.32 -2.0
204,993 - Pf6N2E2_149 0.32 -0.1
204,993 - Pf6N2E2_149 0.32 +1.4
204,993 - Pf6N2E2_149 0.32 +0.6
205,107 - Pf6N2E2_149 0.40 -0.6
205,107 - Pf6N2E2_149 0.40 -1.4

Or see this region's nucleotide sequence