Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1427 and Pf6N2E2_1428 are separated by 305 nucleotides Pf6N2E2_1428 and Pf6N2E2_1429 are separated by 133 nucleotides Pf6N2E2_1429 and Pf6N2E2_1430 are separated by 56 nucleotides
Pf6N2E2_1427 - 6-aminohexanoate-dimer hydrolase (EC 3.5.1.46), at 1,675,677 to 1,676,939
_1427
Pf6N2E2_1428 - Alkanesulfonates-binding protein, at 1,677,245 to 1,678,348
_1428
Pf6N2E2_1429 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 1,678,482 to 1,679,243
_1429
Pf6N2E2_1430 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 1,679,300 to 1,680,625
_1430
Position (kb) 1677
1678
1679 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1676.260 kb on + strand, within Pf6N2E2_1427 at 1676.261 kb on - strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1677.202 kb on + strand at 1677.255 kb on + strand at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.853 kb on - strand, within Pf6N2E2_1428 at 1677.873 kb on - strand, within Pf6N2E2_1428 at 1678.152 kb on - strand, within Pf6N2E2_1428 at 1678.152 kb on - strand, within Pf6N2E2_1428 at 1678.176 kb on + strand, within Pf6N2E2_1428 at 1678.177 kb on - strand, within Pf6N2E2_1428 at 1678.395 kb on + strand at 1678.440 kb on + strand at 1678.441 kb on - strand at 1678.441 kb on - strand at 1678.441 kb on - strand at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1679.106 kb on - strand, within Pf6N2E2_1429 at 1679.195 kb on - strand at 1679.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 1,676,260 + Pf6N2E2_1427 0.46 -0.1 1,676,261 - Pf6N2E2_1427 0.46 -1.6 1,676,440 + Pf6N2E2_1427 0.60 -2.6 1,676,440 + Pf6N2E2_1427 0.60 +1.9 1,676,440 + Pf6N2E2_1427 0.60 -0.4 1,676,441 - Pf6N2E2_1427 0.60 +2.2 1,676,441 - Pf6N2E2_1427 0.60 +0.5 1,677,202 + +2.5 1,677,255 + +1.6 1,677,430 - Pf6N2E2_1428 0.17 +0.7 1,677,430 - Pf6N2E2_1428 0.17 -0.1 1,677,430 - Pf6N2E2_1428 0.17 -1.0 1,677,430 - Pf6N2E2_1428 0.17 -2.3 1,677,708 + Pf6N2E2_1428 0.42 -1.5 1,677,708 + Pf6N2E2_1428 0.42 +2.2 1,677,709 - Pf6N2E2_1428 0.42 -1.2 1,677,709 - Pf6N2E2_1428 0.42 +1.8 1,677,709 - Pf6N2E2_1428 0.42 +0.8 1,677,709 - Pf6N2E2_1428 0.42 -0.2 1,677,732 + Pf6N2E2_1428 0.44 +1.6 1,677,732 + Pf6N2E2_1428 0.44 -1.3 1,677,732 + Pf6N2E2_1428 0.44 +1.4 1,677,733 - Pf6N2E2_1428 0.44 +1.6 1,677,733 - Pf6N2E2_1428 0.44 -1.0 1,677,733 - Pf6N2E2_1428 0.44 +0.9 1,677,780 - Pf6N2E2_1428 0.48 -0.9 1,677,780 - Pf6N2E2_1428 0.48 -0.7 1,677,853 - Pf6N2E2_1428 0.55 +1.1 1,677,873 - Pf6N2E2_1428 0.57 +0.7 1,678,152 - Pf6N2E2_1428 0.82 +1.0 1,678,152 - Pf6N2E2_1428 0.82 +0.1 1,678,176 + Pf6N2E2_1428 0.84 +0.7 1,678,177 - Pf6N2E2_1428 0.84 +1.4 1,678,395 + +0.1 1,678,440 + -1.2 1,678,441 - -2.2 1,678,441 - -0.4 1,678,441 - +1.0 1,678,588 + Pf6N2E2_1429 0.14 -0.2 1,678,588 + Pf6N2E2_1429 0.14 -0.6 1,678,588 + Pf6N2E2_1429 0.14 +0.6 1,678,588 + Pf6N2E2_1429 0.14 -2.1 1,678,589 - Pf6N2E2_1429 0.14 +0.2 1,678,589 - Pf6N2E2_1429 0.14 +1.0 1,678,589 - Pf6N2E2_1429 0.14 -0.4 1,678,589 - Pf6N2E2_1429 0.14 +0.8 1,678,589 - Pf6N2E2_1429 0.14 -0.5 1,678,589 - Pf6N2E2_1429 0.14 -0.9 1,679,106 - Pf6N2E2_1429 0.82 -1.4 1,679,195 - +0.3 1,679,263 + -1.6
Or see this region's nucleotide sequence