Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides Pf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotides Pf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotides
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426
_1310
Position (kb)
1542
1543
1544
1545 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1542.058 kb on - strand, within Pf6N2E2_1307 at 1542.112 kb on + strand at 1542.377 kb on - strand, within Pf6N2E2_1308 at 1542.421 kb on + strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.431 kb on - strand, within Pf6N2E2_1308 at 1542.545 kb on - strand, within Pf6N2E2_1308 at 1542.559 kb on + strand, within Pf6N2E2_1308 at 1542.560 kb on - strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.979 kb on - strand at 1543.308 kb on + strand, within Pf6N2E2_1309 at 1543.318 kb on - strand, within Pf6N2E2_1309 at 1543.360 kb on + strand, within Pf6N2E2_1309 at 1543.361 kb on - strand, within Pf6N2E2_1309 at 1543.501 kb on + strand, within Pf6N2E2_1309 at 1543.565 kb on - strand, within Pf6N2E2_1309 at 1543.908 kb on + strand, within Pf6N2E2_1309 at 1544.136 kb on + strand, within Pf6N2E2_1309 at 1544.151 kb on + strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.301 kb on + strand at 1544.362 kb on + strand at 1544.362 kb on + strand at 1544.389 kb on + strand at 1544.390 kb on - strand at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.842 kb on + strand, within Pf6N2E2_1310 at 1544.965 kb on + strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1545.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 1,541,976 + Pf6N2E2_1307 0.74 +2.8 1,541,976 + Pf6N2E2_1307 0.74 +0.3 1,541,976 + Pf6N2E2_1307 0.74 +3.5 1,541,976 + Pf6N2E2_1307 0.74 +0.1 1,541,976 + Pf6N2E2_1307 0.74 +0.2 1,542,058 - Pf6N2E2_1307 0.87 -0.2 1,542,112 + +3.1 1,542,377 - Pf6N2E2_1308 0.30 +0.8 1,542,421 + Pf6N2E2_1308 0.36 -0.3 1,542,422 - Pf6N2E2_1308 0.36 +3.3 1,542,422 - Pf6N2E2_1308 0.36 -0.8 1,542,431 - Pf6N2E2_1308 0.37 +0.1 1,542,545 - Pf6N2E2_1308 0.52 -2.2 1,542,559 + Pf6N2E2_1308 0.54 -1.9 1,542,560 - Pf6N2E2_1308 0.54 -1.3 1,542,739 + Pf6N2E2_1308 0.77 -0.4 1,542,739 + Pf6N2E2_1308 0.77 -1.3 1,542,979 - +4.1 1,543,308 + Pf6N2E2_1309 0.26 -0.7 1,543,318 - Pf6N2E2_1309 0.27 -0.4 1,543,360 + Pf6N2E2_1309 0.30 -0.9 1,543,361 - Pf6N2E2_1309 0.30 -0.9 1,543,501 + Pf6N2E2_1309 0.40 +0.4 1,543,565 - Pf6N2E2_1309 0.44 -1.8 1,543,908 + Pf6N2E2_1309 0.68 -0.0 1,544,136 + Pf6N2E2_1309 0.83 -1.4 1,544,151 + Pf6N2E2_1309 0.84 +0.6 1,544,152 - Pf6N2E2_1309 0.84 +3.0 1,544,152 - Pf6N2E2_1309 0.84 -0.0 1,544,301 + -0.4 1,544,362 + +1.0 1,544,362 + +2.6 1,544,389 + +0.7 1,544,390 - +1.6 1,544,617 + Pf6N2E2_1310 0.22 +0.2 1,544,617 + Pf6N2E2_1310 0.22 +0.7 1,544,617 + Pf6N2E2_1310 0.22 +1.4 1,544,618 - Pf6N2E2_1310 0.22 +1.8 1,544,618 - Pf6N2E2_1310 0.22 -1.2 1,544,794 + Pf6N2E2_1310 0.39 -0.6 1,544,794 + Pf6N2E2_1310 0.39 +0.4 1,544,795 - Pf6N2E2_1310 0.39 -1.4 1,544,795 - Pf6N2E2_1310 0.39 -0.3 1,544,795 - Pf6N2E2_1310 0.39 +0.7 1,544,837 - Pf6N2E2_1310 0.43 +0.6 1,544,837 - Pf6N2E2_1310 0.43 -2.3 1,544,837 - Pf6N2E2_1310 0.43 -1.4 1,544,842 + Pf6N2E2_1310 0.43 +1.7 1,544,965 + Pf6N2E2_1310 0.55 -0.0 1,544,966 - Pf6N2E2_1310 0.55 -0.3 1,544,966 - Pf6N2E2_1310 0.55 -2.1 1,545,347 - -1.0
Or see this region's nucleotide sequence